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Literature DB >> 3818549

Efficient transfection of the archaebacterium Halobacterium halobium.

Abstract

We developed an efficient polyethylene glycol-mediated spheroplast transfection method for the extremely halophilic archaebacterium Halobacterium halobium. The 59-kilobase-pair linear phage phi H DNA molecule routinely produced between 5 X 10(6) and 2 X 10(7) transfectants per microgram of DNA. Between 0.5 and 1% of spheroplasts were transfected per microgram of luminal diameter H DNA. Under our conditions, survival and regeneration of H. halobium spheroplasts were also quite efficient, suggesting that this method will be useful for introducing other DNAs into these bacteria.

Entities: Chemical Species

Mesh：

Year: 1987 PMID： 3818549 PMCID： PMC211943 DOI： 10.1128/jb.169.3.1341-1344.1987

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

8 in total

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Journal: J Bacteriol Date: 1985-04 Impact factor: 3.490

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Journal: Eur J Biochem Date: 1978-08-15

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Journal: Methods Enzymol Date: 1980 Impact factor: 1.600

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Authors: R J Klebe; J V Harriss; Z D Sharp; M G Douglas
Journal: Gene Date: 1983-11 Impact factor: 3.688

8. Halobacterium halobium phage øH.

Authors: H Schnabel; W Zillig; M Pfäffle; R Schnabel; H Michel; H Delius
Journal: EMBO J Date: 1982 Impact factor: 11.598

8 in total

56 in total

1. Sensory rhodopsin II from the haloalkaliphilic natronobacterium pharaonis: light-activated proton transfer reactions.

Authors: G Schmies; B Lüttenberg; I Chizhov; M Engelhard; A Becker; E Bamberg
Journal: Biophys J Date: 2000-02 Impact factor: 4.033

2. Localizing genes on the map of the genome of Haloferax volcanii, one of the Archaea.

Authors: A Cohen; W L Lam; R L Charlebois; W F Doolittle; L C Schalkwyk
Journal: Proc Natl Acad Sci U S A Date: 1992-03-01 Impact factor: 11.205

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Authors: M L Holmes; M L Dyall-Smith
Journal: J Bacteriol Date: 1990-02 Impact factor: 3.490

4. Protonatable residues at the cytoplasmic end of transmembrane helix-2 in the signal transducer HtrI control photochemistry and function of sensory rhodopsin I.

Authors: K H Jung; J L Spudich
Journal: Proc Natl Acad Sci U S A Date: 1996-06-25 Impact factor: 11.205

5. Characterization of pHV2 from Halobacterium volcanii and its use in demonstrating transformation of an archaebacterium.

Authors: R L Charlebois; W L Lam; S W Cline; W F Doolittle
Journal: Proc Natl Acad Sci U S A Date: 1987-12 Impact factor: 11.205

6. Disruption of a sugar transporter gene cluster in a hyperthermophilic archaeon using a host-marker system based on antibiotic resistance.

Authors: Rie Matsumi; Kenji Manabe; Toshiaki Fukui; Haruyuki Atomi; Tadayuki Imanaka
Journal: J Bacteriol Date: 2007-01-26 Impact factor: 3.490