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Literature DB >> 2825193

Characterization of pHV2 from Halobacterium volcanii and its use in demonstrating transformation of an archaebacterium.

R L Charlebois¹, W L Lam, S W Cline, W F Doolittle.

Abstract

We determined the complete nucleotide sequence of the 6354-base-pair plasmid pHV2 of the archaebacterium Halobacterium volcanii. This plasmid is present in approximately six copies per chromosome. We have generated a strain, H. volcanii WFD11, cured of pHV2 by treatment of liquid cultures with ethidium bromide. We describe PEG-mediated transformation of H. volcanii WFD11 with intact pHV2 and with a form of pHV2 marked by a 93-base-pair deletion generated in vitro.

Entities: Chemical Gene Species

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Year: 1987 PMID： 2825193 PMCID： PMC299578 DOI： 10.1073/pnas.84.23.8530

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

26 in total

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