| Literature DB >> 27310134 |
Matthew T Henke1,2, Alexandra A Soukup1,2, Anthony W Goering1,2, Ryan A McClure1,2, Regan J Thomson1,2, Nancy P Keller1,2, Neil L Kelleher1,2.
Abstract
Unlocking the biochemical stores of fungi is key for developing future pharmaceuticals. Through reduced expression of a critical histone deacetylase in Aspergillus nidulans, increases of up to 100-fold were observed in the levels of 15 new aspercryptins, recently described lipopeptides with two noncanonical amino acids derived from octanoic and dodecanoic acids. In addition to two NMR-verified structures, MS/MS networking helped uncover an additional 13 aspercryptins. The aspercryptins break the conventional structural orientation of lipopeptides and appear "backward" when compared to known compounds of this class. We have also confirmed the 14-gene aspercryptin biosynthetic gene cluster, which encodes two fatty acid synthases and several enzymes to convert saturated octanoic and dodecanoic acid to α-amino acids.Entities:
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Year: 2016 PMID： 27310134 PMCID： PMC5119465 DOI： 10.1021/acschembio.6b00398
Source DB: PubMed Journal: ACS Chem Biol ISSN： 1554-8929 Impact factor: 5.100