Literature DB >> 27064647

Expansion microscopy with conventional antibodies and fluorescent proteins.

Tyler J Chozinski1, Aaron R Halpern1, Haruhisa Okawa2, Hyeon-Jin Kim1, Grant J Tremel1, Rachel O L Wong2, Joshua C Vaughan1,3.   

Abstract

Expansion microscopy is a technique in which fluorophores on fixed specimens are linked to a swellable polymer that is physically expanded to enable super-resolution microscopy with ordinary microscopes. We have developed and characterized new methods for linking fluorophores to the polymer that now enable expansion microscopy with conventional fluorescently labeled antibodies and fluorescent proteins. Our methods simplify the procedure and expand the palette of compatible labels, allowing rapid dissemination of the technique.

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Year:  2016        PMID： 27064647      PMCID： PMC4929147          DOI： 10.1038/nmeth.3833

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  11 in total

Review 1.  Far-field optical nanoscopy.

Authors:  Stefan W Hell
Journal:  Science       Date:  2007-05-25       Impact factor: 47.728

2.  Proteins coupled to polyacrylamide beads using glutaraldehyde.

Authors:  P D Weston; S Avrameas
Journal:  Biochem Biophys Res Commun       Date:  1971-12-17       Impact factor: 3.575

3.  Breaking the diffraction barrier: super-resolution imaging of cells.

Authors:  Bo Huang; Hazen Babcock; Xiaowei Zhuang
Journal:  Cell       Date:  2010-12-23       Impact factor: 41.582

4.  Incorporation of adhesion peptides into nonadhesive hydrogels useful for tissue resurfacing.

Authors:  D L Hern; J A Hubbell
Journal:  J Biomed Mater Res       Date:  1998-02

5.  Optical imaging. Expansion microscopy.

Authors:  Fei Chen; Paul W Tillberg; Edward S Boyden
Journal:  Science       Date:  2015-01-15       Impact factor: 47.728

Review 6.  Glutaraldehyde: behavior in aqueous solution, reaction with proteins, and application to enzyme crosslinking.

Authors:  Isabelle Migneault; Catherine Dartiguenave; Michel J Bertrand; Karen C Waldron
Journal:  Biotechniques       Date:  2004-11       Impact factor: 1.993

7.  Single-cell phenotyping within transparent intact tissue through whole-body clearing.

Authors:  Bin Yang; Jennifer B Treweek; Rajan P Kulkarni; Benjamin E Deverman; Chun-Kan Chen; Eric Lubeck; Sheel Shah; Long Cai; Viviana Gradinaru
Journal:  Cell       Date:  2014-07-31       Impact factor: 41.582

8.  Autofluorescence generation and elimination: a lesson from glutaraldehyde.

Authors:  Kwahun Lee; Sungmoon Choi; Chun Yang; Hai-Chen Wu; Junhua Yu
Journal:  Chem Commun (Camb)       Date:  2013-04-14       Impact factor: 6.222

9.  Structural and molecular interrogation of intact biological systems.

Authors:  Kwanghun Chung; Jenelle Wallace; Sung-Yon Kim; Sandhiya Kalyanasundaram; Aaron S Andalman; Thomas J Davidson; Julie J Mirzabekov; Kelly A Zalocusky; Joanna Mattis; Aleksandra K Denisin; Sally Pak; Hannah Bernstein; Charu Ramakrishnan; Logan Grosenick; Viviana Gradinaru; Karl Deisseroth
Journal:  Nature       Date:  2013-04-10       Impact factor: 49.962

10.  Resolution doubling in 3D-STORM imaging through improved buffers.

Authors:  Nicolas Olivier; Debora Keller; Pierre Gönczy; Suliana Manley
Journal:  PLoS One       Date:  2013-07-17       Impact factor: 3.240
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  103 in total

1.  Basics of Expansion Microscopy.

Authors:  Aleksandra Klimas; Brendan Gallagher; Yongxin Zhao
Journal:  Curr Protoc Cytom       Date:  2019-12

2.  Expansion microscopy of C. elegans.

Authors:  Chih-Chieh Jay Yu; Nicholas C Barry; Asmamaw T Wassie; Anubhav Sinha; Abhishek Bhattacharya; Shoh Asano; Chi Zhang; Fei Chen; Oliver Hobert; Miriam B Goodman; Gal Haspel; Edward S Boyden
Journal:  Elife       Date:  2020-05-01       Impact factor: 8.140

Review 3.  Expansion microscopy: development and neuroscience applications.

Authors:  Emmanouil D Karagiannis; Edward S Boyden
Journal:  Curr Opin Neurobiol       Date:  2018-01-06       Impact factor: 6.627

4.  Molecular organization of mammalian meiotic chromosome axis revealed by expansion STORM microscopy.

Authors:  Huizhong Xu; Zhisong Tong; Qing Ye; Tengqian Sun; Zhenmin Hong; Lunfeng Zhang; Alexandra Bortnick; Sunglim Cho; Paolo Beuzer; Joshua Axelrod; Qiongzheng Hu; Melissa Wang; Sylvia M Evans; Cornelis Murre; Li-Fan Lu; Sha Sun; Kevin D Corbett; Hu Cang
Journal:  Proc Natl Acad Sci U S A       Date:  2019-08-23       Impact factor: 11.205

Review 5.  Advanced imaging and labelling methods to decipher brain cell organization and function.

Authors:  Daniel Choquet; Matthieu Sainlos; Jean-Baptiste Sibarita
Journal:  Nat Rev Neurosci       Date:  2021-03-12       Impact factor: 34.870

6.  Analyzing Centrioles and Cilia by Expansion Microscopy.

Authors:  Dong Kong; Jadranka Loncarek
Journal:  Methods Mol Biol       Date:  2021

7.  Effects of laser polarization on responses of the fluorescent Ca2+ indicator X-Rhod-1 in neurons and myelin.

Authors:  Ileana Micu; Craig Brideau; Li Lu; Peter K Stys
Journal:  Neurophotonics       Date:  2017-06-05       Impact factor: 3.593

Review 8.  Photostable and photoswitching fluorescent dyes for super-resolution imaging.

Authors:  Masafumi Minoshima; Kazuya Kikuchi
Journal:  J Biol Inorg Chem       Date:  2017-01-12       Impact factor: 3.358

9.  Corrigendum: The tetrameric kinesin Kif25 suppresses pre-mitotic centrosome separation to establish proper spindle orientation.

Authors:  Justin Decarreau; Michael Wagenbach; Eric Lynch; Aaron R Halpern; Joshua C Vaughan; Justin Kollman; Linda Wordeman
Journal:  Nat Cell Biol       Date:  2017-05-31       Impact factor: 28.824

10.  Three-Dimensional Single-Molecule Localization Microscopy in Whole-Cell and Tissue Specimens.

Authors:  Sheng Liu; Hyun Huh; Sang-Hyuk Lee; Fang Huang
Journal:  Annu Rev Biomed Eng       Date:  2020-04-03       Impact factor: 9.590
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