Literature DB >> 23462727

Autofluorescence generation and elimination: a lesson from glutaraldehyde.

Kwahun Lee1, Sungmoon Choi, Chun Yang, Hai-Chen Wu, Junhua Yu.   

Abstract

Glutaraldehyde causes especially high autofluorescence. It reacted with proteins and peptides to generate visible to near-IR emitters. A model indicated that ethylenediamine and a secondary amine in the molecule were key components for the formation of emissive species. The mechanism enables us to control the generation and elimination of autofluorescence.

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Year:  2013        PMID： 23462727     DOI： 10.1039/c3cc40799c

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  13 in total

1.  Interplay of Genotype and Substrate Stiffness in Driving the Hypertrophic Cardiomyopathy Phenotype in iPSC-Micro-Heart Muscle Arrays.

Authors:  Jingxuan Guo; Huanzhu Jiang; Kasoorelope Oguntuyo; Brandon Rios; Zoë Boodram; Nathaniel Huebsch
Journal:  Cell Mol Bioeng       Date:  2021-06-25       Impact factor: 3.337

2.  Expansion microscopy with conventional antibodies and fluorescent proteins.

Authors:  Tyler J Chozinski; Aaron R Halpern; Haruhisa Okawa; Hyeon-Jin Kim; Grant J Tremel; Rachel O L Wong; Joshua C Vaughan
Journal:  Nat Methods       Date:  2016-04-11       Impact factor: 28.547

3.  Differential Translocation of Host Cellular Materials into the Chlamydia trachomatis Inclusion Lumen during Chemical Fixation.

Authors:  Marcela Kokes; Raphael H Valdivia
Journal:  PLoS One       Date:  2015-10-01       Impact factor: 3.240

4.  Quantification of protein mobility and associated reshuffling of cytoplasm during chemical fixation.

Authors:  Jan Huebinger; Jessica Spindler; Kristin J Holl; Björn Koos
Journal:  Sci Rep       Date:  2018-12-10       Impact factor: 4.379

5.  An improved method for preparation of mouse retinal cryosections.

Authors:  Ling Li; Yongqiong Lin; Fang Yang; Tongdan Zou; Jialiang Yang; Houbin Zhang
Journal:  Eur J Histochem       Date:  2020-09-03       Impact factor: 3.188

6.  Comparison of Different Tissue Clearing Methods for Three-Dimensional Reconstruction of Human Brain Cellular Anatomy Using Advanced Imaging Techniques.

Authors:  Marina Scardigli; Luca Pesce; Niamh Brady; Giacomo Mazzamuto; Vladislav Gavryusev; Ludovico Silvestri; Patrick R Hof; Christophe Destrieux; Irene Costantini; Francesco S Pavone
Journal:  Front Neuroanat       Date:  2021-11-11       Impact factor: 3.856

7.  Exploring the human cerebral cortex using confocal microscopy.

Authors:  Luca Pesce; Annunziatina Laurino; Marina Scardigli; Jiarui Yang; David A Boas; Patrick R Hof; Christophe Destrieux; Irene Costantini; Francesco Saverio Pavone
Journal:  Prog Biophys Mol Biol       Date:  2021-09-16       Impact factor: 3.667

8.  A Biocompatible and Biodegradable Protein Hydrogel with Green and Red Autofluorescence: Preparation, Characterization and In Vivo Biodegradation Tracking and Modeling.

Authors:  Xiaoyu Ma; Xiangcheng Sun; Derek Hargrove; Jun Chen; Donghui Song; Qiuchen Dong; Xiuling Lu; Tai-Hsi Fan; Youjun Fu; Yu Lei
Journal:  Sci Rep       Date:  2016-01-27       Impact factor: 4.379

9.  A Phenotyping Method of Giant Cells from Root-Knot Nematode Feeding Sites by Confocal Microscopy Highlights a Role for CHITINASE-LIKE 1 in Arabidopsis.

Authors:  Javier Cabrera; Rocio Olmo; Virginia Ruiz-Ferrer; Isidro Abreu; Christian Hermans; Isabel Martinez-Argudo; Carmen Fenoll; Carolina Escobar
Journal:  Int J Mol Sci       Date:  2018-02-01       Impact factor: 5.923

10.  Flow cytometry detection of surface and intracellular antigens in pancreas from a single mouse embryo.

Authors:  Pia Nyeng; Gelo Victoriano Dela Cruz; Henrik Semb
Journal:  STAR Protoc       Date:  2021-06-30
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