| Literature DB >> 26686630 |
Rajesha Rupaimoole1, Jaehyuk Lee2, Monika Haemmerle3, Hui Ling4, Rebecca A Previs3, Sunila Pradeep3, Sherry Y Wu3, Cristina Ivan5, Manuela Ferracin6, Jennifer B Dennison7, Niki M Zacharias Millward2, Archana S Nagaraja1, Kshipra M Gharpure1, Michael McGuire1, Nidhin Sam3, Guillermo N Armaiz-Pena3, Nouara C Sadaoui1, Cristian Rodriguez-Aguayo8, George A Calin8, Ronny I Drapkin9, Jeffery Kovacs10, Gordon B Mills7, Wei Zhang11, Gabriel Lopez-Berestein8, Pratip K Bhattacharya2, Anil K Sood12.
Abstract
Long noncoding RNAs (lncRNAs) significantly influence the development and regulation of genome expression in cells. Here, we demonstrate the role of lncRNA ceruloplasmin (NRCP) in cancer metabolism and elucidate functional effects leading to increased tumor progression. NRCP was highly upregulated in ovarian tumors, and knockdown of NRCP resulted in significantly increased apoptosis, decreased cell proliferation, and decreased glycolysis compared with control cancer cells. In an orthotopic mouse model of ovarian cancer, siNRCP delivered via a liposomal carrier significantly reduced tumor growth compared with control treatment. We identified NRCP as an intermediate binding partner between STAT1 and RNA polymerase II, leading to increased expression of downstream target genes such as glucose-6-phosphate isomerase. Collectively, we report a previously unrecognized role of the lncRNA NRCP in modulating cancer metabolism. As demonstrated, DOPC nanoparticle-incorporated siRNA-mediated silencing of this lncRNA in vivo provides therapeutic avenue toward modulating lncRNAs in cancer.Entities:
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Year: 2015 PMID： 26686630 PMCID： PMC4691557 DOI： 10.1016/j.celrep.2015.11.047
Source DB: PubMed Journal: Cell Rep Impact factor: 9.423