Shrunken Pore Syndrome is associated with a sharp rise in mortality in patients undergoing elective coronary artery bypass grafting.

Shrunken Pore Syndrome was recently suggested for the pathophysiologic state in patients characterized by an estimation of their glomerular filtration rate (GFR) based upon cystatin  C, which is lower or equal to 60% of their estimated GFR based upon creatinine, i.e. when eGFR cystatin  C ≤ 60% of eGFR creatinine. Not only the cystatin C level, but also the levels of other low molecular mass proteins are increased in this condition. The preoperative plasma levels of cystatin C and creatinine were measured in 1638 patients undergoing elective coronary artery bypass grafting. eGFR cystatin C and eGFR creatinine were calculated using two pairs of estimating equations, CAPA and LMrev, and CKD-EPI cystatin  C and CKD-EPI creatinine, respectively. The Shrunken Pore Syndrome was present in 2.1% of the patients as defined by the CAPA and LMrev equations and in 5.7% of the patients as defined by the CKD-EPI cystatin C and CKD-EPI creatinine equations. The patients were studied over a median follow-up time of 3.5 years (2.0-5.0 years) and the mortality determined. Shrunken Pore Syndrome defined by both pairs of equations was a strong, independent, predictor of long-term mortality as evaluated by Cox analysis and as illustrated by Kaplan-Meier curves. Increased mortality was observed also for the subgroups of patients with GFR above or below 60 mL/min/1.73 m(2). Changing the cut-off level from 60 to 70% for the CAPA and LMrev equations increased the number of patients with Shrunken Pore Syndrome to 6.5%, still displaying increased mortality.

Introduction

A decrease in GFR signals increased risks for development of end stage renal disease (ESRD), cardiovascular disease, hospitalization and death and GFR-estimations based upon cystatin  C are consistently superior to GFR-estimations based upon creatinine to predict these conditions [1-3]. The cause for the superiority of cystatin  C as a risk marker is unknown, but it has been suggested that inflammation raises the cystatin  C level, thereby augmenting its potential as a risk marker [4]. However, studies of elective surgery on patients displayed a sharp rise in inflammation of the patients with large increases in the levels of CRP and other inflammatory markers, but with no increase in the level of cystatin  C, thus allowing rejection of this hypothesis [5]. Another hypothesis for the superiority of cystatin  C as a risk marker is that an increase in cystatin  C, with a molecular mass of 13343 Da, signals a shrinking of the pores in the glomerular membrane earlier than an increase in creatinine with a molecular mass of 113 Da [6]. Studies of the plasma levels of cystatin  C and other low molecular mass proteins, e.g. β2-microglobulin and beta-trace protein, in late pregnancy and in pre-eclampsia have produced results, which are compatible with the shrinking of glomerular pores in these conditions [7-10]. A recent study of these proteins, in a general patient population, strongly indicated that a significant part of the population suffers from shrunken glomerular pores similar to the situation in late pregnancy and pre-eclampsia [11]. It was observed that if the estimation of GFR based upon cystatin  C was lower or equal to 60% of the estimation of GFR based upon creatinine, i.e. when eGFRcystatin  C ≤ 60% of eGFRcreatinine, all other low molecular mass proteins studied, like cystatin  C, displayed increased levels and this constellation of eGFRcystatin  C and eGFRcreatinine, was therefore suggested to indicate the presence of a new syndrome called ‘Shrunken Pore Syndrome’ [11]. The present study was undertaken to investigate if the presence of the Shrunken Pore Syndrome in a well-characterized and large cohort of patients undergoing elective coronary artery bypass grafting (CABG) predicts long-term mortality.

Materials

The plasma levels of cystatin  C and creatinine were measured preoperatively in 1638 patients undergoing CABG from 1 January to 31 December 2010, at the Department of Cardiothoracic Surgery at Skåne University Hospital in Lund, Sweden. The study was approved by the local ethics committee (LU 2009/87). The characteristics of the patient population pre- and postoperatively are described in Tables I and II.

Table I.

Preoperative characteristics of the study cohort (n = 1638).

Variable	Mean (±SD)/ No. (%)
Age (year)	67.4 (± 9.6)
Female gender	343 (21%)
Body Mass Index (kg/m2)	27.5 (± 3.8)
Diabetes	405 (25%)
COPD	180 (11%)
eGFR (mL/min/1.73 m2) according to: CAPA LMrev CKD-EPIcystatin C CKD-EPIcreatinine Anemia	67 (± 19)70 (± 17)68 (± 22)78 (± 19)142 (9%)
Previous vascular surgery	81 (5%)
Peripheral vascular disease	212 (13%)
History of cerebrovascular injury	150 (9%)
LVEF 30–50%	338 (21%)
LVEF < 30%	115 (7%)
Previous myocardial infarction	798 (49%)
Euro score – additive	4.3 (±3.1)
NYHA class
I	522 (32%)
II	527 (32%)
III	472 (29%)
IV	117 (7%)
CCS class
I	201 (12%)
II	750 (46%)
III	525 (32%)
IV	162 (10%)
IABP before surgery	21 (1%)
Preoperative hemoglobin (g/L)	136.4 (± 15.0)

Presented as either mean (± standard deviation) for continuous variables or number (percent) for dichotomous variables. CCS, Canadian Cardiovascular Society; COPD, chronic obstructive pulmonary disease; IABP, intra-aortic balloon pump; LVEF, left ventricular ejection fraction; NYHA, New York Heart Association.

Table II.

Postoperative characteristics of the study cohort (n = 1638).

Outcomes	Mean (± SD)/No. (%)
Use of CBP	1638 (100%)
Perfusion time (min)	77.2 (± 26.2)
Cross clamp time (min)	46.1 (± 17.8)
IABP after surgery	23 (0.014%)
Time in the ICU (h)	23 (20–27)*
Sepsis	10 (0.6%)
Myocardial infarction	8 (0.5%)
Permanent stroke	4 (0.2)%
Pneumonia	18 (1.1%)
RRT	10 (0.6%)
Atrial fibrillation	321 (19.6%)
Time in ventilator (min)	300 (210–430)*
Re-operated for bleeding	33 (2.0%)
Re-operated for mediastinitis	14 (0.9%)
Erythrocytes transfused (units)	1.2 (± 2.6)
Plasma transfused (units)	0.51 (± 2.4)
Platelets transfused (units)	0.22 (± 0.84)

Presented as either mean (± standard deviation) for continuous variables, number (percent) for dichotomous variables, or as median (interquartile range) for outcome variables that are skewed*. CBP, cardiopulmonary bypass; IABP, intra-aortic balloon pump; ICU, Intensive care unit; RRT, renal replacement therapy.

Perioperative data was obtained from an in-house quality database. Laboratory data were continuously obtained from the hospital clinical chemistry department. Survival data were obtained from the national tax registry in December 2014, thus defining the follow-up period between 2 and 5 years with a mean follow-up of 3.5 years.

Methods

The plasma level of cystatin  C was determined by an automated particle-based immunoassay, adjusted to the international reference preparation ERM-DA 471/IFCC [12] and that of creatinine by an enzymatic colorimetric assay with an IDMS-traceable calibrator [13]. Both assays were run on a cobas c-system (Roche Diagnostics, Basel, Switzerland). The assays were performed according to the manufacturer’s instructions. The CAPA [12] and the CKD-EPIcystatin  C [14] estimating equations, based on cystatin  C, were used to estimate GFR. So were the LMrev [13] and CKD-EPIcreatinine [14] estimating equations based on creatinine. The best estimate of GFR is the mean of eGFRcystatin  C and of eGFRcreatinine (eGFRmean) and this is true also when eGFRcystatin  C and eGFRcreatinine differ significantly, unless known disturbing factors like muscle wasting or treatment with large doses of glucocorticoids are present [13-17]. Such disturbing factors were present preoperatively in very few of the patients undergoing CABG.

Statistical analysis

The variables are presented as absolute numbers with percentages, mean with standard deviation or median with interquartile ranges (25th and 75th percentile). Survival rates during follow-up were estimated by Kaplan-Meier curves and comparisons were made by log-rank test. The Cox multivariate proportional hazards model was used to determine independent predictors for mortality. The variables entered in the Cox proportional hazards regression model were age, gender, body mass index, chronic obstructive pulmonary disease preoperatively, preoperative hemoglobin, creatinine, leukocyte particle count, diabetes, instable angina pectoris, previous cerebrovascular lesion, peripheral vascular disease, ejection fraction, New York Heart association class, use of intra-aortic balloon pump both pre- and postoperatively, perfusion-time, atrial fibrillation both pre- and postoperatively, postoperative bleeding, sepsis, logarithm of time in ventilator, logarithm of units of plasma transfusion, erythrocyte transfusion, platelet transfusion, mediastinitis, postoperative stroke and renal replacement therapy. A backward stepwise elimination method yielded the following risk factors as independent predictors for mortality after CABG presented in detail in Table III: age, peripheral vascular disease, left ventricular function (reference EF > 30%), time spent in the ICU, preoperative hemoglobin, sepsis, logarithm of units of plasma transfused and the pre-operative renal function as estimated by equations based upon combined values of cystatin  C and creatinine, e.g. CAPA-LMrev or CKD-EPIcystatin  C-CKD-EPIcreatinine [12-14]. These significant predictors of mortality were used in the final model. This model was used for determining the independent effect of the Shrunken Pore Syndrome by adding this variable to the model. The adjusted Hazard Ratio (HR) and 95% confidence intervals (95% CI) were calculated for Shrunken Pore Syndrome as a predictor; p-values < 0.05 (two-tailed) were considered statistically significant. Statistical analysis was performed with Statistica software, version 12.0 (Stat Soft, Inc. Tulsa, OK, USA).

Table III.

Cox multivariate analysis of patients with and without Shrunken Pore Syndrome. (A) Defined by the equation pair CKD-EPIcystatin C and CKD-EPIcreatinine with a cut-off of 60% (i.e. eGFRcystatin C ≤ 0.6 eGFRcreatinine). (B) Defined by the equation pair CAPA and LMrev with a cut-off of 60% (i.e. eGFRcystatin C ≤ 0.6 eGFRcreatinine). (C) Defined by the equation pair CAPA and LMrev with a cut-off of 70% (i.e. eGFRcystatin C ≤ 0.7 eGFRcreatinine).

	(A) CKD-EPI, cut-off 0.6			(B) CAPA-LMrev, cut-off 0.6			(C) CAPA-LMrev, cut-off 0.7
	p-value	HR	95% CI	p-value	HR	95% CI	p-value	HR	95% CI
Age (per year)	0.004588	1.036	1.011–1.062	0.001181	1.042	1.016–1.068	0.000745	1.044	1.018–1.070
Peripheral vascular disease	0.004756	1.825	1.202–2.771	0.008423	1.751	1.154–2.657	0.003787	1.852	1.220–2.811
LVEF< 30%	0.110203	1.487	0.914–2.419	0.077655	1.552	0.952–2.530	0.155834	1.426	0.873–2.329
Hours in ICU	0.004740	1.003	1.001–1.005	0.012124	1.002	1.001–1.004	0.004223	1.003	1.001–1.005
Preoperative Hemoglobin (g/L)	0.004137	0.981	0.969–0.994	0.001112	0.979	0.966–0.991	0.004042	0.981	0.968–0.994
Log units of plasma given	0.000009	1.701	1.345–2.151	0.000001	1.826	1.448–2.302	0.000002	1.763	1.399–2.221
Preoperative eGFRmean (mL/min/1.73 m2)	0.000003	0.975	0.965–0.985	0.000018	0.975	0.963–0.986	0.000024	0.975	0.963–0.986
Shrunken Pore Syndrome	0.000016	2.742	1.733–4.339	0.000166	3.445	1.810–6.557	0.000006	2.941	1.842–4.694

HR, hazard ratio; CI, confidence interval; LVEF, left ventricle ejection fraction; ICU, Intensive care unit.

Results

The Shrunken Pore Syndrome was identified both by the equation pair CAPA and LMrev and by the equation pair CKD-EPIcystatin  C and CKD-EPIcreatinine in this study. The prevalence of preoperative Shrunken Pore Syndrome was 2.1% (34/1638) and 5.7% (93/1638), respectively. The 2- and 5-year survival for all patients was (mean ± 95% CI) 96.1 ± 0.4% and 90.0 ± 1.2%, respectively. Late survival for patients with Shrunken Pore Syndrome as defined by the equation pair CAPA and LMrev was 82.4 ± 6.5% and 58.8 ± 9.0% at 2 and 5 years, respectively. Late survival for patients with Shrunken Pore Syndrome as defined by the equation pair CKD-EPIcystatin  C and CKD-EPIcreatinine was 81.7 ± 0.4% and 65.1 ± 0.6% at 2 and 5 years, respectively.

Shrunken Pore Syndrome was found to be a significant predictor for mortality in the Cox analysis when the equation pair CKD-EPIcystatin  C and CKD-EPIcreatinine was used (Table III). The cohort was divided into subgroups of patients with eGFRmean < 60 mL/min/1.73 m2 or eGFRmean ≥ 60 mL/min/1.73 m2 and the associations between survival, and the presence of Shrunken Pore Syndrome preoperatively, were studied. Strong associations (Table III), and a marked decrease in survival, were found for both subgroups. The survival rates are illustrated by the Kaplan-Meier curves in Figure 1.

Figure 1.

Calculations using the CKD-EPI formulas based on cystatin C or creatinine. Survival after coronary artery bypass surgery for patients with eGFR > 60 mL/min/1.73 m2 (A) with Shrunken Pore Syndrome (SPS, red broken line) and without (blue solid line). Patients with eGFR< 60 mL/min/1.73 m2 are seen in (B). The cut-off level for SPS was 0.6. For both levels of eGFR: p < 0.001 with log-rank test.

Shrunken Pore Syndrome was also found to be a significant predictor for mortality in the Cox analysis when the equation pair CAPA and LMrev was used (Table III). This cohort was also divided into subgroups of patients with eGFRmean < 60 mL/min/1.73 m2 and eGFRmean ≥ 60 mL/min/1.73 m2, and the associations between survival, and the presence of Shrunken Pore Syndrome preoperatively, were studied. Strong associations, and a marked decrease in survival, were found for both subgroups (Table III). The survival rates are illustrated by the Kaplan-Meier curves in Figure 2.

Figure 2.

Calculations using the CAPA and LMrev formulas based on cystatin C or creatinine. Survival after coronary artery bypass surgery for patients with eGFR > 60 mL/min/1.73 m2 (A) with Shrunken Pore Syndrome (SPS, red broken line) and without (blue solid line). Patients with eGFR < 60 mL/min/1.73 m2 are seen in (B). The cut-off level for SPS was 0.6. For both levels of eGFR: p < 0.001 with log-rank test.

The number of patients (34) identified as suffering from the Shrunken Pore Syndrome preoperatively by the equation pair CAPA and LMrev was considerably lower than that (93) identified by the equation pair CKD-EPIcystatin  C and CKD-EPIcreatinine, although the same criterion for Shrunken Pore Syndrome (eGFRcystatin  C ≤ 60% of eGFRcreatinine) was used in both cases. This was most probably due to that the CKD-EPIcreatinine estimation equation produced significantly higher GFR estimates than the other three estimation equations in the patient cohort studied. The average GFR value for the cohort was 78 mL/min/1.73 m2 as estimated by CKD-EPIcreatinine, whereas the averages estimated by CKD-EPIcystatin  C, LMrev and CAPA were 68, 70 and 67 mL/min/1.73 m2, respectively (Table I). This means that smaller changes in estimates based upon CKD-EPIcystatin  C are required to reach the criterion of eGFRcystatin  C ≤ 60% of eGFRcreatinine, when the equation pair CKD-EPIcystatin  C and CKD-EPIcreatinine is used. To compare the potential of the two equation pairs to identify patients with a significant increase in mortality, we therefore increased the cut-off level defining Shrunken Pore Syndrome by the equation pair CAPA and LMrev to eGFRcystatin  C ≤ 70% of eGFRcreatinine. With this cut-off level, 106 patients (6.5%) were defined as suffering from the Shrunken Pore Syndrome. The association between survival, and the presence of Shrunken Pore Syndrome preoperatively, as identified by the equation pair CAPA and LMrev with the new cut-off level was then studied by Cox multivariate analysis for the whole patient cohort. A strong, statistically significant, association was observed (Table III). The cohort was divided into subgroups of patients with eGFRmean < 60 mL/min/1.73 m2 and eGFRmean ≥ 60 mL/min/1.73 m2 and the associations between survival, and the presence of Shrunken Pore Syndrome preoperatively, were studied. Strong associations, and a marked decrease in survival, were found for both subgroups also in this case (Table III). The survival rates are illustrated by the Kaplan-Meier curves in Figure 3.

Figure 3.

Calculations using the CAPA and LMrev formulas based on cystatin C or creatinine. Survival after coronary artery bypass surgery for patients with eGFR > 60 mL/min/1.73 m2 (A) with Shrunken Pore Syndrome (SPS, red broken line) and without (blue solid line). Patients with eGFR < 60 mL/min/1.73 m2 are seen in (B). The cut-off level for SPS was 0.7. For both levels of eGFR: p < 0.001 with log-rank test.

Although the numbers of patients with Shrunken Pore Syndrome (SPS+), displayed in Figures 1–3, were few at the 4- and 5-year follow-up, the Kaplan-Meier curves show that mortality starts to separate earlier than this period, when the numbers of patients with Shrunken Pore Syndrome were higher.

When the patient groups identified as suffering from the Shrunken Pore Syndrome preoperatively by the equation pair CKD-EPIcystatin  C and CKD-EPIcreatinine, using the cut-off level of 60% and by the equation pair CAPA and LMrev, using the new cut-off level of 70%, were compared, it was found that 82 of the 93 patients were found also in the group of patients defined as suffering from the Shrunken Pore Syndrome by the equation pair CAPA and LMrev, using the new cut-off level. When the patient groups identified as suffering from the Shrunken Pore Syndrome preoperatively by the equation pair CAPA and LMrev, using the new cut-off level and by the equation pair CKD-EPIcystatin  C and CKD-EPIcreatinine, using the cut-off level of 60% were compared, it was found that 82 of the 106 patients were found also in the group of patients defined as suffering from the Shrunken Pore Syndrome by the equation pair CKD-EPIcystatin  C and CKD-EPIcreatinine, using the cut-off level of 60%. Table IV summarizes the number of patients defined as suffering from Shrunken Pore Syndrome by the three different constellations of GFR-estimating equations.

Table IV.

Number of patients defined as suffering from ‘Shrunken Pore Syndrome’ by different GFR-estimating equation constellations.

Estimating equation constellation	Number of patients with Shrunken Pore Syndrome
(I) CKD-EPIcystatin C ≤ 60% of CKD-EPIcreatinine	93, of which 82 were also identified by equation constellation III
(II) CAPA ≤ 60% of LMrev	34, of which 33 and 34 were also identified by equation constellations I and III, respectively
(III) CAPA ≤ 70% of LMrev	106, of which 82 were also identified by equation constellation I

Discussion

The existence of a Shrunken Pore Syndrome was suggested from a recent study of 1349 consecutive patients, 731 men and 618 women, between 3 and 95 years of age, for which eGFR was requested at the department of clinical chemistry [11]. To estimate GFR both the LMrev-equation, based upon creatinine, and the CAPA-equation, based upon cystatin  C, were used and the Shrunken Pore Syndrome was defined by eGFRcystatin  C being less or equal to 60% of eGFRcreatinine in a patient [11]. However, the clinical implications of the presence of the Shrunken Pore Syndrome were not investigated. The present study of a well characterized population of 1638 patients scheduled for elective CABG demonstrated that the presence of the Shrunken Pore Syndrome preoperatively was independently associated with a marked increase in late mortality.

The present study found a Hazard Ratio of approximately 3 for Shrunken Pore Syndrome, which indicates the ominous nature of this syndrome. Patients with low BMI have lower muscle mass, and thus lower creatinine values, than patients with normal or high BMI, and a high proportion of patients with low BMI in a population might therefore influence the number of patients defined as suffering from Shrunken Pore Syndrome. We therefore excluded the 25 patients with BMI < 20 kg/m2 from the population studied and repeated the statistical analysis. There was no reduction in hazard ratio (HR) values for presence of the Shrunken Pore Syndrome after excluding patients with BMI < 20 kg/m2. The presence of patients with low BMI therefore seems to have little influence upon the clinical consequences of Shrunken Pore Syndrome.

It should be observed that the present study is limited to a select patient category, and an urgent task is, of course, to study the association between the syndrome and its consequences in other populations, and with other end-points than mortality.

The cut-off of eGFRcystatin  C ≤ 60% of eGFRcreatinine, suggested for definition of the syndrome, was chosen from the eGFR values frequently occurring in pre-eclampsia [11], but is, of course, somewhat arbitrary and may not be the most efficient for identification of risk individuals in neither the population used to define the syndrome; nor in other types of patient populations. To specify a suitable cut-off in a specific type of population would require that the population is very big so that the occurrence of significant clinical consequences can be studied at various cut-off levels. The present population of patients undergoing CABG is not big enough to allow such a study, but may be expanded to allow it.

A further problem in establishing a suitable cut-off level defining the syndrome is that different GFR-estimating equations may produce different values. For example, in the present patient cohort the CKD-EPIcreatinine-equation produced estimations of GFR which were about 10% higher than the estimates produced by the CKD-EPIcystatin  C-, CAPA- and LMrev-equations, which showed mutually consistent results. This means that smaller changes in estimates based upon CKD-EPIcystatin  C are required to reach the criterion of eGFRcystatin  C ≤ 60% of eGFRcreatinine, when the equation pair CKD-EPIcystatin  C and CKD-EPIcreatinine is used rather than the equation pair CAPA and LMrev. To compare the potential of the two equation pairs to identify patients with a significant increase in mortality as a results of the presence of the Shrunken Pore Syndrome, we increased the cut-off level defining Shrunken Pore Syndrome by the equation pair CAPA and LMrev to eGFRcystatin  C ≤ 70% of eGFRcreatinine, resulting in 106 patients (6.5%) being identified as suffering from the syndrome. Also in this group of patients a strong, statistically significant, increase in late mortality was observed, both in patients with eGFRmean below and above 60 mL/min/1.73 m2 (Table III, Figure 3). The population identified was almost identical with the population defined by the original cut-off level of 60% in the equation pair CKD-EPIcystatin  C and CKD-EPIcreatinine. These results elucidate some of the problems of selecting a suitable cut-off level to identify individuals suffering from the Shrunken Pore Syndrome.

The Cox multivariate analysis demonstrated that the preoperative presence of Shrunken Pore Syndrome independently predicts poorer survival in addition to previously known risk factors like age, preoperative hemoglobin level, presence of peripheral vascular disease and preoperative eGFR (Table III) [18]. A remarkable finding is that the hazard ratios (2.7–3.4), defined by the three different constellations of equation pairs used in this investigation, are considerably higher than those of some well known risk-factors such as peripheral vascular disease (HR 1.8) and depressed left ventricular ejection fraction (HR 1.5) on the Cox analysis. Whether this severe nature of the Shrunken Pore Syndrome holds true for other patient cohorts needs to be explored.

The use of eGFRcystatin  C ≤ 60% of eGFRcreatinine to define the presence or absence of Shrunken Pore Syndrome can be replaced by the use of the ratio: eGFRcystatin  C/eGFRcreatinine ≤ 0.6. Since the decisive terms in eGFRcystatin  C and eGFRcreatinine are cystatin  C and creatinine, respectively, the cystatin  C/creatinine-ratio will also be related to the presence of the putative Shrunken Pore Syndrome and to late mortality in the present population. However, the GFR-estimating equations are more closely related to GFR, than isolated cystatin  C- and creatinine-levels, and our statistical analyses have not been able to display any advantage of using the cystatin  C/creatinine-ratio, rather than the relation eGFRcystatin  C ≤ 60% of eGFRcreatinine, to reveal an increase in late mortality in our population. However, our population is relatively small and homogeneous and the results may be different for subgroups in bigger and more heterogeneous populations.

Comparison of eGFRcystatin  C and eGFRcreatinine is easy to use for identification of a putative Shrunken Pore Syndrome. A more rigorous way to identify shrunken pores is to use invasive procedures in which substances, only being excreted by glomerular filtration, and of different molecular sizes, are injected and their plasma or renal clearance determined. The polysaccharides dextran and Ficoll of great polydispersity have been extensively used in animals to demonstrate a reduction or an increase in glomerular pore diameter in various pathophysiological states. There are also a few studies in humans [19-26], in which these substances have been used to demonstrate the presence of shrunken or widened glomerular pores, but the laborious procedures and accompanying ethical aspects will probably not allow their general use in health care.

Despite the work remaining to find suitable cut-off levels for identifying the Shrunken Pore Syndrome, it seems clear that the syndrome indicates a pathophysiological state with severe clinical consequences. Future challenges are to elucidate the etiology and pathophysiology of the syndrome and to study if the development of the syndrome can be stopped or delayed. It is possible that the pathophysiology of Shrunken Pore Syndrome is related to that of pre-eclampsia and thus involves endothelial dysfunctions [27]. Since pre-eclampsia is reversible and can be treated, this might also be true for Shrunken Pore Syndrome [27].