| Literature DB >> 26555057 |
Leonid Peshkin1, Martin Wühr2, Esther Pearl3, Wilhelm Haas4, Robert M Freeman1, John C Gerhart5, Allon M Klein1, Marko Horb3, Steven P Gygi6, Marc W Kirschner7.
Abstract
A biochemical explanation of development from the fertilized egg to the adult requires an understanding of the proteins and RNAs expressed over time during embryogenesis. We present a comprehensive characterization of protein and mRNA dynamics across early development in Xenopus. Surprisingly, we find that most protein levels change little and duplicated genes are expressed similarly. While the correlation between protein and mRNA levels is poor, a mass action kinetics model parameterized using protein synthesis and degradation rates regresses protein dynamics to RNA dynamics, corrected for initial protein concentration. This study provides detailed data for absolute levels of ∼10,000 proteins and ∼28,000 transcripts via a convenient web portal, a rich resource for developmental biologists. It underscores the lasting impact of maternal dowry, finds surprisingly few cases where degradation alone drives a change in protein level, and highlights the importance of transcription in shaping the dynamics of the embryonic proteome.Entities:
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Year: 2015 PMID： 26555057 PMCID： PMC4776761 DOI： 10.1016/j.devcel.2015.10.010
Source DB: PubMed Journal: Dev Cell ISSN： 1534-5807 Impact factor: 12.270