Literature DB >> 31772075

Transcriptomics and Proteomics Methods for Xenopus Embryos and Tissues.

Michael J Gilchrist1,2, Gert Jan C Veenstra1,2, Ken W Y Cho3.   

Abstract

The general field of quantitative biology has advanced significantly on the back of recent improvements in both sequencing technology and proteomics methods. The development of high-throughput, short-read sequencing has revolutionized RNA-based expression studies, while improvements in proteomics methods have enabled quantitative studies to attain better resolution. Here we introduce methods to undertake global analyses of gene expression through RNA and protein quantification in Xenopus embryos and tissues.
© 2020 Cold Spring Harbor Laboratory Press.

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Year:  2020        PMID： 31772075      PMCID： PMC7362671          DOI： 10.1101/pdb.top098350

Source DB:  PubMed          Journal:  Cold Spring Harb Protoc        ISSN： 1559-6095


  24 in total

1.  Deep proteomics of the Xenopus laevis egg using an mRNA-derived reference database.

Authors:  Martin Wühr; Robert M Freeman; Marc Presler; Marko E Horb; Leonid Peshkin; Steven Gygi; Marc W Kirschner
Journal:  Curr Biol       Date:  2014-06-19       Impact factor: 10.834

2.  INTACT Proteomics in Xenopus.

Authors:  Lauren Wasson; Nirav M Amin; Frank L Conlon
Journal:  Cold Spring Harb Protoc       Date:  2019-06-03

3.  A novel role for sox7 in Xenopus early primordial germ cell development: mining the PGC transcriptome.

Authors:  Amanda M Butler; Dawn A Owens; Lingyu Wang; Mary Lou King
Journal:  Development       Date:  2018-01-08       Impact factor: 6.868

4.  Single-cell mass spectrometry reveals small molecules that affect cell fates in the 16-cell embryo.

Authors:  Rosemary M Onjiko; Sally A Moody; Peter Nemes
Journal:  Proc Natl Acad Sci U S A       Date:  2015-05-04       Impact factor: 11.205

5.  Label-free Quantification of Proteins in Single Embryonic Cells with Neural Fate in the Cleavage-Stage Frog (Xenopus laevis) Embryo using Capillary Electrophoresis Electrospray Ionization High-Resolution Mass Spectrometry (CE-ESI-HRMS).

Authors:  Camille Lombard-Banek; Sushma Reddy; Sally A Moody; Peter Nemes
Journal:  Mol Cell Proteomics       Date:  2016-06-17       Impact factor: 5.911

6.  On the Relationship of Protein and mRNA Dynamics in Vertebrate Embryonic Development.

Authors:  Leonid Peshkin; Martin Wühr; Esther Pearl; Wilhelm Haas; Robert M Freeman; John C Gerhart; Allon M Klein; Marko Horb; Steven P Gygi; Marc W Kirschner
Journal:  Dev Cell       Date:  2015-11-09       Impact factor: 12.270

7.  Assessing the performance of the Oxford Nanopore Technologies MinION.

Authors:  T Laver; J Harrison; P A O'Neill; K Moore; A Farbos; K Paszkiewicz; D J Studholme
Journal:  Biomol Detect Quantif       Date:  2015-03

8.  Molecular asymmetry in the 8-cell stage Xenopus tropicalis embryo described by single blastomere transcript sequencing.

Authors:  Elena De Domenico; Nick D L Owens; Ian M Grant; Rosa Gomes-Faria; Michael J Gilchrist
Journal:  Dev Biol       Date:  2015-06-19       Impact factor: 3.582

9.  A catalog of Xenopus tropicalis transcription factors and their regional expression in the early gastrula stage embryo.

Authors:  Ira L Blitz; Kitt D Paraiso; Ilya Patrushev; William T Y Chiu; Ken W Y Cho; Michael J Gilchrist
Journal:  Dev Biol       Date:  2016-07-28       Impact factor: 3.582

10.  Genome-wide analysis of dorsal and ventral transcriptomes of the Xenopus laevis gastrula.

Authors:  Yi Ding; Gabriele Colozza; Kelvin Zhang; Yuki Moriyama; Diego Ploper; Eric A Sosa; Maria D J Benitez; Edward M De Robertis
Journal:  Dev Biol       Date:  2016-03-23       Impact factor: 3.582
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  1 in total

1.  Genomics Methods for Xenopus Embryos and Tissues.

Authors:  Michael J Gilchrist; Ken W Y Cho; Gert Jan C Veenstra
Journal:  Cold Spring Harb Protoc       Date:  2020-05-01
  1 in total

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