Cellular Mechanisms of Drosophila Heart Morphogenesis.

Many of the major discoveries in the fields of genetics and developmental biology have been made using the fruit fly, Drosophila melanogaster. With regard to heart development, the conserved network of core cardiac transcription factors that underlies cardiogenesis has been studied in great detail in the fly, and the importance of several signaling pathways that regulate heart morphogenesis, such as Slit/Robo, was first shown in the fly model. Recent technological advances have led to a large increase in the genomic data available from patients with congenital heart disease (CHD). This has highlighted a number of candidate genes and gene networks that are potentially involved in CHD. To validate genes and genetic interactions among candidate CHD-causing alleles and to better understand heart formation in general are major tasks. The specific limitations of the various cardiac model systems currently employed (mammalian and fish models) provide a niche for the fly model, despite its evolutionary distance to vertebrates and humans. Here, we review recent advances made using the Drosophila embryo that identify factors relevant for heart formation. These underline how this model organism still is invaluable for a better understanding of CHD.

1. Introduction

Congenital heart disease (CHD) is the most frequently diagnosed birth defect, with an incident rate of about 1% among newborns [1,2]. There are several genetic origins of CHD, such as copy-number variation (CNV), which affects gene dosage (as found in DiGeorge Syndrome and Williams–Beuren Syndrome), or mutations that affect a single gene, often a cardiac transcription factor (i.e., NKX2.5, GATA4) or structural components of the contractile apparatus of the cardiomyocytes (such as MYH6; for a review, see [3,4]). However, the underlying genetics of the majority of cases of CHD (which are labeled “sporadic”) seems to be more complex and is likely caused by the interaction of independent alleles and loci (“polygenic”). While recent advances in sequencing technology have led to a drastic increase in the data available on genetic variants among patients’ genomes, it is still necessary to experimentally validate candidate genes that were computationally derived from such genomic data.

Understanding the biological basis of CHD requires a molecular understanding of the complex development of the heart itself, and despite the great efforts of the scientific community, our understanding of heart morphogenesis is still very incomplete. Current vertebrate models of heart development are mouse, chicken and zebrafish embryos (for reviews, see [5,6,7,8]), which are complemented by cell-based assays, including heart tissue engineered from induced pluripotent stem cells (iPSCs) [9]. These models established valuable concepts for the number and origin of cardiac progenitor cells, lineage restriction and the respective contributions of these cells to different regions of the heart. To study the complexity of the heart and heart morphogenesis, it is necessary to combine different approaches to complement the specific shortcomings of each model. This need has spurred the introduction and adoption of other models, such as tunicates [10], which are basic chordates, and even invertebrate model organisms, such as the fruit fly, Drosophila melanogaster [11,12,13,14].

In the last three decades, the powerful genetic model system, Drosophila, has been instrumental in the identification and dissection of the genetics of many developmental processes, including heart development (for a review, see [15]), serving as a pioneer for higher model organisms. Drosophila is an extremely versatile model, with a plethora of reagents and genetic tools available, including RNAi lines to tissue-specific targeting of each fly gene [16]. One particular advantage of the fly model is that heart-specific genetic manipulations of critical factors of heart development still allow its survival into adulthood and permit functional analysis, while the same approach in higher model organisms often causes embryonic lethality. In a reverse-genetic screen for genes affecting adult heart function under stress conditions, several members of the CCR4-Not complex were identified to cause dilated cardiomyopathy in the fly, a function that is conserved in a murine model and with implications for human heart disease [17]. One recent example is the role of the YAK/Hippo pathway in mammalian cardiovascular development [18]. Originally identified in Drosophila, YAK/Hippo plays a major role in tissue size control, and recent data show that this function seems to be conserved in mammals. Therefore, lessons learned from Drosophila can be (re-)applied to improve our understanding of human heart development and might even be helpful in identifying strategies for preventing and repairing cardiac dysfunction. Here, we review recent advances in the understanding of the molecular-genetic control of heart morphogenesis in Drosophila.

2. Genetics of Drosophila Heart Formation

2.1. Genetic Control of Cardiac Specification

The embryonic Drosophila heart is a simple, tubular organ comprised of two different cell types: the cardioblasts (CBs), which differentiate into contractile cardiomyocytes of the “working myocardium” and ostia cells, that form the inflow tracts of the heart; and pericardial cells (PCs), most of which become nephrocyte-like cells involved in ultrafiltration that line the outside of the heart tube [19,20]. Pericardial cells have a dual role of also serving as ROS-sensors for the myocardium [21]. The precursors of both the CBs and PCs are specified in the dorsal mesoderm, under the influence of ectodermal TGFβ and WNT signaling pathways [22]. This “cardiac mesoderm” contains the heart’s founder cells that will divide and give rise to both pure and mixed lineages of CBs and PCs (and somatic muscle cells). At this stage, a number of cell fate decisions are made, leading to the specification of subtypes of CBs and PCs, which is under the control of the Notch signaling pathway and its regulators, such as Sanpodo, Numb and ADAM metalloproteinase-disintegrins [22,23,24,25,26]. The identification of Tinman [27,28], a homeobox transcription factor, led to the identification of its mammalian homologue, Nkx2.5, as the first essential factor for the specification of the cardiogenic region in both flies and vertebrates. The genetics and specification cascade downstream of tin function in Drosophila has been described in great detail (for a detailed review, see Bodmer and Frasch [15]). Briefly, Tinman and the TBX transcription factor, Dorsocross-1/2/3 [29], induce expression of the GATA4-homologue Pannier in the dorsal mesoderm, where Pannier acts as a permissive factor for CB specification [30]. Later, during development, Tinman is subsequently required for the further specification and differentiation of CBs [31], in part through activation of Mef2 [32]. Other examples of cardiac transcription factors studied in Drosophila include HAND [33], Neuromancer1/2-TBX20 [34,35], Tail-up/Islet-1 [36,37] and the COUP transcription factor, Seven-up [38]. This genetic and mutational interrogation of cardiogenesis was recently complemented by ChIP-on-chip studies of whole embryos [39,40], which globally identified the transcriptional activity and regulatory networks controlled by Tinman and several other cardiac transcription factors.

2.2. Genetics of Heart Morphogenesis

Once specified, the cardiac precursor cells migrate towards the dorsal midline, where they undergo changes in cell shape and assemble to form a linear heart tube. While the bilateral origin of the heart precursors and their fusion at the midline resembles early vertebrate heart morphogenesis (i.e., heart cone formation; compare [41,42]), the linear arrangement of ipsilateral CBs along the anterior-posterior axis and the two-cell composition of the cardiac lumen appear to be morphologically distinct and more similar to mammalian blood vessels than the mammalian heart (e.g., developing mouse aorta [43]). In Drosophila, the CBs migrate and undergo morphogenesis in about five hours, which is experimentally advantageous, as it allows cell behavior to be studied in real time throughout heart formation. The CBs and heart are also very accessible for confocal time-lapse and fluorescence imaging of stained whole-mount embryos, since these cells are localized just below the epidermis, which is a single-cell layer and, thus, semi-transparent.

Once the CBs have migrated to the midline, they establish dorsal contacts with the contralateral counterpart, then undergo cell shape changes and ventrally contact the other CBs, thereby enclosing a central lumen. This occurs in a “zipper-like” fashion that closes from both the anterior and posterior ends. The Drosophila heart tube is compartmentalized along the A/P axis. A cardiac outflow tract (OFT) is already formed anteriorly while the bilateral heart cells are closing [44], whereas the posterior region of the heart, the “heart proper” forms a larger heart lumen. The CBs in this section differentiate into contractile cardiomyocytes and pairs of cells that form inflow tracts (ostia cells; see [45]). The “aortic” region of the heart is located between the OFT and heart proper and is characterized by the much smaller diameter of the heart lumen and the lack of ostia cells. The heart itself is physically connected to a number of other cell types that also directly contribute to heart function, specifically the alary muscle cells [46,47], which may anchor the heart tube to the epidermis, and the nephrocyte-like pericardial cells [21,48,49,50]. Extracellular matrix proteins, such as laminins and collagens (including Pericardin and Multiplexin), localize in and around the heart and are required for heart assembly and integrity [51,52,53,54,55]. Signaling proteins, such as Integrins [56], as well as G proteins [57], regulate cardiac ECM and cell adhesion directly or indirectly.

Once such pathways are uncovered in Drosophila, homologous pathways can be investigated in higher model organisms. For example, studies on the role of the Slit-Robo signaling pathway in Drosophila heart morphogenesis have triggered the analysis of homologous genes in vertebrate model organisms. Early on, it was noticed that the ligand Slit is expressed in fly CBs [58]. Subsequent mutational analysis of slit and the genes encoding its receptors robo and robo2 revealed that this pathway is indeed critical for maintaining CB chain integrity during migration [59] and for CB cell shape changes during heart morphogenesis [60,61,62]. In vertebrates, Slit/Robo has been implicated in zebrafish heart tube formation [63], where slit2 is necessary for the migration of endocardial cells during heart tube formation, and in robo1/2 and slit3 mutant mouse models, where the formation of the pericardial and venous return is defective [64]. How the Slit-Robo signaling cascade controls morphogenetic events was first worked out for post-mitotic neurons, as they migrate during Drosophila nervous system development: binding of the signaling molecule Slit to its receptor Robo induces recruitment of Son-of-Sevenless, a guanine nucleotide exchange factor (GEF), which, in turn, activates the Rho family of GTPases that control the cytoskeletal changes required for the specific migratory response of the neuronal growth cone [65]. The simple Drosophila heart tube is well suited to allow the identification of the factors downstream of Slit-Robo, which, in turn, become candidate genes to be analyzed in higher model organisms. Subsequent analyses of the downstream effectors of Slit-Robo using Drosophila will likely reveal additional candidate genes to be tested in these models. For example, the Drosophila gene encoding Multiplexin (Mp, a collagen15/18) was recently identified to modify Slit-Robo signaling and potentiating Slit activity, resulting in reduced formation of F-actin and altered heart lumen size [54].

2.3. Analyzing the Actomyosin Network during Drosophila Heart Morphogenesis

Underneath the instructive layer of cardiac transcription factors and signaling molecules are those proteins that transduce these cues into cellular behavior. For example, CB-specific mutation of tinman causes misspecification of cells and morphogenetic defects that are a combination of cell fate changes and inappropriate cell behavior [31].

Elucidating how the cells interpret these internal and external cues is fundamental not only for a comprehensive understanding of organogenesis, but it also offers additional, alternate and specific therapeutic avenues by targeting the effector molecules of disease genes. These basic aspects of cell biology are usually studied in cell culture, where high-resolution analysis of cellular effectors is possible. However, cell culture lacks the complexity of differentiated, three-dimensional multicellular environments, which are present in a living embryo. Therefore, the simplicity of the developing Drosophila heart is advantageous to analyze cardiac cell biology during heart morphogenesis and to eventually explain how signaling pathways, such as Slit/Robo and Integrin signaling, give rise to specific cardiac phenotypes when mutated.

Members of the small Rho GTPase family are common molecular hubs that control a large variety of cellular responses, such as cell polarization or cytoskeletal organization (for a review, see [66,67]). Interestingly, the small GTPase Cdc42 was previously identified as a genetic interactor with tinman in adult hearts of Drosophila and mice [68]. This interaction was also manifest in the Drosophila embryo during heart morphogenesis [69], indicating that Tinman might control heart morphogenesis through Cdc42. During heart development, Cdc42 is necessary for Tin-positive cardioblasts to complete dorsal migration [70], and it controls CB cell shape changes during heart lumen formation. Interestingly, both processes appear to be specific for the small Rho-GTPase Cdc42, but not for the other members, such as Rho1 or Rac1 [69].

Lumen formation in Drosophila occurs by enclosing of a central lumen, where the CBs actively bend around the luminal space (see Figure 1). Such changes in cell shape are achieved by the activity of the actomyosin network, which has not been studied in detail in the context of Drosophila heart formation. Throughout dorsal closure, actomyosin is active in epidermal cells (as “purse-string” actin-myosin cables that pull epidermal sheets towards the midline) and in the cells of the amnioserosa, an extra-embryonic layer that degenerates at late stages of development and, thereby, creates tension by reducing surface area.

Figure 1

(a) Overview of Drosophila heart morphogenesis. Cardioblasts (CBs, green circles) collectively migrate towards the dorsal midline of the embryo. These differentiate later into contractile cardiomyocytes (light green) and ostia cells (dark green). Actomyosin activity can be detected at the edge of the cells of the amnioserosa (AS), at the leading edge of the epidermis (LE) and at the apical side of CBs (red arrows indicate the axis of actomyosin bundles). CB migration is completed within 5 h. Epithelial closure precedes heart closure, and once the CBs make first contact at Stage 16, within 30 min, they actively enclose a central lumen. At that stage, several compartments within the CBs can be identified using several different markers. (b) Model showing genetic interactions between Cdc42, formins, actomyosin and the luminal proteins, Slit and Multiplexin. The upstream regulators of Cdc42 (both positive and negative) are yet to be identified.

Analysis of the expression pattern of the Drosophila non-muscle myosin-II Zipper (Zip) revealed a specific and very dynamic pattern throughout heart morphogenesis [69]. During Stage 15–16, before dorsal closure, Zip repetitively accumulates at the apical side of the CBs, which may be important for apical constriction of CBs to support dorsal closure. During lumen formation, Zip temporarily accumulates at the luminal surface to maintain CB/lumen curvature. In CBs, non-muscle myosin II dynamics is positively regulated by Cdc42 and formin activity, with Cdc42 also showing genetic interaction with zip and the formin, dDAAM1 [69]. Interestingly, cardiac laterality during zebrafish heart morphogenesis depends on the control of non-muscle myosin-II by BMP signaling [71]. This highlights the need for a better understanding of how the actomyosin network functions in vivo and how it is spatio-temporally regulated.

While these aspects of actomyosin regulation might relate to how force generation shapes the heart, Cdc42/formins also control the heart lumen in a more general fashion. The activation of Cdc42 and formins is sufficient to relocate the entire set of known lumen markers, such as Dystroglycan (Dg), Perlecan/Trol, Slit and Multiplexin, to newly formed, ectopic heart lumina. The latter proteins are part of the extracellular matrix (including anchoring proteins, like Dg, or signaling molecules, like Slit; for an overview, see [72]), and they carry out specific functions during heart morphogenesis and lumen formation, including the downregulation of F-actin itself [54]. In addition, Cdc42 controls the migration of Tin-positive cells before dorsal closure and independent of the known Cdc42 downstream effector p21-activated kinase. The number and hierarchy of genes involved in heart morphogenesis that mediate this cellular behavior are still unknown. In addition, the upstream signals controlling CB migration and lumen formation have yet to be identified. While different signaling pathways (Slit-Robo, Netrin-Unc5, Integrins) are required for heart formation, the molecular-genetic pathways mediating the cellular responses have not been found. In fact, neither Slit-Robo, nor Netrin-Unc5 mutants show changes in non-muscle myosin dynamics [69], which indicates that other pathways are involved, as well. Furthermore, the interaction between CBs and the surrounding tissues (amnioserosa, pericardial cells, dorsal ectoderm) is likely to be critical for heart morphogenesis, as well, but to date, very little is known about how these tissues interact. Recent work showed that regulation of lipid phosphates, Wunen and Wunen2, expressed in the developing heart, is necessary for heart morphogenesis [73], as well as for the timing of dorsal closure, which is delayed in wun/wun2 mutants.

With the advent of affordable whole-genome sequencing, forward genetic screens (such as EMS screens, e.g., in [74]) to identify new players in heart development or genetic interactors of known cardiac factors become more feasible again. In addition, improved RNAi-based approaches, as well as targeted mutations using CRISPR/Cas9 [75,76,77] will allow one to model candidate genes identified from human patients in the fly model and to subsequently functionally evaluate these variants and test for genetic interaction. CRISPR/Cas9 also permits rapid analysis of single genes in zebrafish and mouse models (e.g., see [78]), and these model organisms have a much smaller evolutionary distance to humans compared to flies. Genetic variants that might be responsible for CHD might not be present in the fly genome and, therefore, are a limitation to the analysis of potentially disease-causing alleles. However, the study of genetic interaction (i.e., with core cardiac factors) is still a domain of the fruit fly due to the lack of genetic redundancy for the vast majority of Drosophila genes compared to vertebrates, which (in addition to many other advantages; see [79]) makes the fruit fly a promising model for CHD research.

3. Conclusions

Drosophila is a powerful tool with which to study the genetic and cellular mechanisms of heart formation. Significant genetic homology between flies and humans means that many of the signaling pathways and gene networks uncovered in the fruit fly are relevant in human patients (for an overview see Table 1). The reverse is likely to also be true. Therefore, the fly heart model can be used to screen through candidate CHD-causing genes.

Table 1

List of selected Drosophila genes regulating heart development.

Drosophila Gene	Class	Selected Ref.	Vertebrate Ortholog(s)	Selected Ref.
Diaphanous	Formin	[69]	DRF3	[10]
Zipper	Non-muscle myosin	[69]	NMHC-II A/B/C	[43,81]
Netrin	Signaling molecule	[82,83]	NET1/3	[84]
Slit	Signaling molecule	[59,60,61,62,69,85]	SLIT1/2/3	[64,86]
Decapentaplegic	Signaling molecule	[87]	BMPs	[88]
Wingless	Signaling molecule	[89]	WNTs	[90]
Frazzled	Signaling Receptor	[83]	NEO1	[91]
heartless	Signaling Receptor	[92]	FGFR3/4	[93]
Notch	Signaling Receptor	[25]	NOTCH1/2/3	[94]
Robo1/2/3	Signaling Receptor	[59,61,62,85]	ROBO1/2/3/4	[64]
Unc-5	Signaling Receptor	[82]	UNC5A/B/C/D	[95]
Kuzbanian	ADAM10 metalloprotease	[23]	ADAM10	[96]
Cdc42	Small Rho GTPase	[69,70]	CDC42	[68,97]
Multiplexin	Collagen type XV/XVIII	[54]	COL15/18	[98,99]
dHand	Transcription factor	[33,100,101]	HAND1/2	[102,103]
DMef2	Transcription factor	[32,104,105]	MEF2A/C/D	[106,107]
Dorsocross1/2/3	Transcription factor	[29]	TBX2/3/5	[108,109,110]
Ladybird-early/late	Transcription factor	[111,112]	Lbx1/2/3	[113]
Neuromancer1/2	Transcription factor	[34,35,114]	TBX20	[115,116]
Pannier	Transcription factor	[30,117,118]	GATA4/6	[119]
Seven-up	Transcription factor	[38,45]	COUP-TF II	[120]
Tail-up	Transcription factor	[36,37,121]	ISL1	[122]
Tinman	Transcription factor	[27,28]	NKX2.5	[123]

The interactions of Cdc42 with downstream effectors, such as formins, as well as feedback from ECM and signaling pathways therefore represents an experimental entry point to study the cellular events leading to organ formation. Dysregulation of these pathways is likely to contribute to CHD, while understanding the cellular mechanism of heart morphogenesis is necessary for the repair and the engineering of cardiac tissue [80].