| Literature DB >> 26095252 |
Morgan L Truitt1, Crystal S Conn1, Zhen Shi2, Xiaming Pang1, Taku Tokuyasu3, Alison M Coady3, Youngho Seo4, Maria Barna5, Davide Ruggero6.
Abstract
eIF4E, the major cap-binding protein, has long been considered limiting for translating the mammalian genome. However, the eIF4E dose requirement at an organismal level remains unexplored. By generating an Eif4e haploinsufficient mouse, we found that a 50% reduction in eIF4E expression, while compatible with normal development and global protein synthesis, significantly impeded cellular transformation. Genome-wide translational profiling uncovered a translational program induced by oncogenic transformation and revealed a critical role for the dose of eIF4E, specifically in translating a network of mRNAs enriched for a unique 5' UTR signature. In particular, we demonstrate that the dose of eIF4E is essential for translating mRNAs that regulate reactive oxygen species, fueling transformation and cancer cell survival in vivo. Our findings indicate eIF4E is maintained at levels in excess for normal development that are hijacked by cancer cells to drive a translational program supporting tumorigenesis.Entities:
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Year: 2015 PMID： 26095252 PMCID： PMC4491046 DOI： 10.1016/j.cell.2015.05.049
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582