Jiadi Xu1,2, Qin Qin1,2, Dan Wu3, Jun Hua1,2, Xiaolei Song1, Michael T McMahon1,2, Frances J Northington4, Jiangyang Zhang1, Peter C M van Zijl1,2, James J Pekar1,2. 1. Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA. 2. F.M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Institute, Baltimore, Maryland, USA. 3. Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA. 4. Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
Abstract
PURPOSE: A steady pulsed imaging and labeling (SPIL) scheme is proposed to obtain high-resolution multislice perfusion images of mice brain using standard preclinical MRI equipment. THEORY AND METHODS: The SPIL scheme repeats a pulsed arterial spin labeling (PASL) module together with a short mixing time to extend the temporal duration of the generated PASL bolus to the total experimental time. Multislice image acquisition takes place during the mixing times. The mixing time is also used for magnetization recovery following image acquisition. The new scheme is able to yield multislice perfusion images rapidly. The perfusion kinetic curve can be measured by a multipulsed imaging and labeling (MPIL) scheme, i.e., acquiring single-slice ASL signals before reaching steady-state in the SPIL sequence. RESULTS: When applying the SPIL method to normal mice, and to mice with unilateral ischemia, high-resolution multislice (five slices) CBF images could be obtained in 8 min. Perfusion data from ischemic mice showed clear CBF reductions in ischemic regions. The SPIL method was also applied to postmortem mice, showing that the method is free from magnetization transfer confounds. CONCLUSION: The new SPIL scheme provides for robust measurement of CBF with multislice imaging capability in small animals.
PURPOSE: A steady pulsed imaging and labeling (SPIL) scheme is proposed to obtain high-resolution multislice perfusion images of mice brain using standard preclinical MRI equipment. THEORY AND METHODS: The SPIL scheme repeats a pulsed arterial spin labeling (PASL) module together with a short mixing time to extend the temporal duration of the generated PASL bolus to the total experimental time. Multislice image acquisition takes place during the mixing times. The mixing time is also used for magnetization recovery following image acquisition. The new scheme is able to yield multislice perfusion images rapidly. The perfusion kinetic curve can be measured by a multipulsed imaging and labeling (MPIL) scheme, i.e., acquiring single-slice ASL signals before reaching steady-state in the SPIL sequence. RESULTS: When applying the SPIL method to normal mice, and to mice with unilateral ischemia, high-resolution multislice (five slices) CBF images could be obtained in 8 min. Perfusion data from ischemicmice showed clear CBF reductions in ischemic regions. The SPIL method was also applied to postmortem mice, showing that the method is free from magnetization transfer confounds. CONCLUSION: The new SPIL scheme provides for robust measurement of CBF with multislice imaging capability in small animals.
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