| Literature DB >> 25512341 |
Xichen Bao1, Haitao Wu2, Xihua Zhu2, Xiangpeng Guo1, Andrew P Hutchins3, Zhiwei Luo1, Hong Song3, Yongqiang Chen3, Keyu Lai3, Menghui Yin4, Lingxiao Xu5, Liang Zhou6, Jiekai Chen3, Dongye Wang7, Baoming Qin8, Jon Frampton9, Hung-Fat Tse10, Duanqing Pei11, Huating Wang12, Biliang Zhang4, Miguel A Esteban13.
Abstract
Recent studies have boosted our understanding of long noncoding RNAs (lncRNAs) in numerous biological processes, but few have examined their roles in somatic cell reprogramming. Through expression profiling and functional screening, we have identified that the large intergenic noncoding RNA p21 (lincRNA-p21) impairs reprogramming. Notably, lincRNA-p21 is induced by p53 but does not promote apoptosis or cell senescence in reprogramming. Instead, lincRNA-p21 associates with the H3K9 methyltransferase SETDB1 and the maintenance DNA methyltransferase DNMT1, which is facilitated by the RNA-binding protein HNRNPK. Consequently, lincRNA-p21 prevents reprogramming by sustaining H3K9me3 and/or CpG methylation at pluripotency gene promoters. Our results provide insight into the role of lncRNAs in reprogramming and establish a novel link between p53 and heterochromatin regulation.Entities:
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Year: 2014 PMID： 25512341 PMCID： PMC4650593 DOI： 10.1038/cr.2014.165
Source DB: PubMed Journal: Cell Res ISSN： 1001-0602 Impact factor: 25.617