| Literature DB >> 29431736 |
Xichen Bao1,2, Xiangpeng Guo1,2, Menghui Yin3, Muqddas Tariq1,2,4, Yiwei Lai1,2,4, Shahzina Kanwal1,2, Jiajian Zhou5, Na Li1,2,6, Yuan Lv1,2,4, Carlos Pulido-Quetglas7, Xiwei Wang1,2, Lu Ji5, Muhammad J Khan1,2,8, Xihua Zhu1,2, Zhiwei Luo1,2,4, Changwei Shao9, Do-Hwan Lim9, Xiao Liu10, Nan Li11, Wei Wang12, Minghui He13, Yu-Lin Liu14, Carl Ward1,2, Tong Wang15, Gong Zhang15, Dongye Wang1,2,16, Jianhua Yang17, Yiwen Chen18, Chaolin Zhang19, Ralf Jauch16, Yun-Gui Yang20, Yangming Wang21, Baoming Qin1, Minna-Liisa Anko22, Andrew P Hutchins23, Hao Sun5, Huating Wang5, Xiang-Dong Fu9, Biliang Zhang3, Miguel A Esteban1,2.
Abstract
We combine the labeling of newly transcribed RNAs with 5-ethynyluridine with the characterization of bound proteins. This approach, named capture of the newly transcribed RNA interactome using click chemistry (RICK), systematically captures proteins bound to a wide range of RNAs, including nascent RNAs and traditionally neglected nonpolyadenylated RNAs. RICK has identified mitotic regulators amongst other novel RNA-binding proteins with preferential affinity for nonpolyadenylated RNAs, revealed a link between metabolic enzymes/factors and nascent RNAs, and expanded the known RNA-bound proteome of mouse embryonic stem cells. RICK will facilitate an in-depth interrogation of the total RNA-bound proteome in different cells and systems.Entities:
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Year: 2018 PMID： 29431736 PMCID： PMC5967874 DOI： 10.1038/nmeth.4595
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547