Yijun Hu1, Haijiang Lin2, Bernard Dib2, Alp Atik2, Peggy Bouzika2, Christopher Lin2, Yueran Yan2, Shibo Tang3, Joan W Miller2, Demetrios G Vavvas2. 1. State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong, 510060, China and Department of Ophthalmology, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, MA 02114, USA. 2. Department of Ophthalmology, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, MA 02114, USA. 3. Aier School of Ophthalmology, Central South University, Changsha, Hunan, 410015, China.
Abstract
PURPOSE: To investigate the expression of inflammatory cytokines in ARPE-19 cells after stimulation with cholesterol crystals. METHODS: APRE-19 cells were cultured, primed with IL-1α, and treated with cholesterol crystals under different concentrations. Inflammatory cytokines (mature-IL-1β, IL-6, and IL-8) in supernatant and inflammatory cytokines (pro-IL-1β, IL-18) in cell lysate were detected by western blot. The NF-κB pathway inhibitor BAY 11-7082 was used to determine the pathway of cytokine expression. RESULTS: Cholesterol crystals did not induce the nucleotide-binding domain leucine-rich repeat containing family, pyrin domain containing 3 (NLRP3) inflammasome, but did increase pro-IL-1β expression in ARPE-19 cells. Cholesterol crystals increased pro-IL-1β expression by activating the NF-κB pathway. Cholesterol crystal activation of the NF-κB pathway also leads to increased IL-6 and IL-8 expression. CONCLUSION: Cholesterol crystals can induce inflammatory cytokine expression in ARPE-19 cells by activating the NF-κB pathway.
PURPOSE: To investigate the expression of inflammatory cytokines in ARPE-19 cells after stimulation with cholesterol crystals. METHODS: APRE-19 cells were cultured, primed with IL-1α, and treated with cholesterol crystals under different concentrations. Inflammatory cytokines (mature-IL-1β, IL-6, and IL-8) in supernatant and inflammatory cytokines (pro-IL-1β, IL-18) in cell lysate were detected by western blot. The NF-κB pathway inhibitor BAY 11-7082 was used to determine the pathway of cytokine expression. RESULTS:Cholesterol crystals did not induce the nucleotide-binding domain leucine-rich repeat containing family, pyrin domain containing 3 (NLRP3) inflammasome, but did increase pro-IL-1β expression in ARPE-19 cells. Cholesterol crystals increased pro-IL-1β expression by activating the NF-κB pathway. Cholesterol crystal activation of the NF-κB pathway also leads to increased IL-6 and IL-8 expression. CONCLUSION:Cholesterol crystals can induce inflammatory cytokine expression in ARPE-19 cells by activating the NF-κB pathway.
Authors: Ronald Klein; Karen J Cruickshanks; Scott D Nash; Elizabeth M Krantz; F Javier Nieto; Guan H Huang; James S Pankow; Barbara E K Klein Journal: Arch Ophthalmol Date: 2010-06
Authors: Yvonne Baumer; Sara McCurdy; Xueting Jin; Tina M Weatherby; Amit K Dey; Nehal N Mehta; Jonathan K Yap; Howard S Kruth; William A Boisvert Journal: Atherosclerosis Date: 2019-06-12 Impact factor: 5.162
Authors: Bernard Dib; Haijiang Lin; Daniel E Maidana; Bo Tian; John B Miller; Peggy Bouzika; Joan W Miller; Demetrios G Vavvas Journal: Biochim Biophys Acta Date: 2015-08-21
Authors: Chunjuan Song; Sayak K Mitter; Xiaoping Qi; Eleni Beli; Haripriya V Rao; Jindong Ding; Colin S Ip; Hongmei Gu; Debra Akin; William A Dunn; Catherine Bowes Rickman; Alfred S Lewin; Maria B Grant; Michael E Boulton Journal: PLoS One Date: 2017-02-21 Impact factor: 3.240
Authors: Rayne R Lim; Margaret E Wieser; Rama R Ganga; Veluchamy A Barathi; Rajamani Lakshminarayanan; Rajiv R Mohan; Dean P Hainsworth; Shyam S Chaurasia Journal: Int J Mol Sci Date: 2020-01-30 Impact factor: 5.923