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Literature DB >> 25047833

Methylation by NSun2 represses the levels and function of microRNA 125b.

Shuai Yuan¹, Hao Tang¹, Junyue Xing¹, Xiuqin Fan¹, Xiaoyu Cai¹, Qiu Li¹, Pei Han¹, Yuhong Luo², Zhuojun Zhang¹, Bin Jiang¹, Yali Dou³, Myriam Gorospe⁴, Wengong Wang⁵.

Abstract

Methylation is a prevalent posttranscriptional modification of RNAs. However, whether mammalian microRNAs are methylated is unknown. Here, we show that the tRNA methyltransferase NSun2 methylates primary (pri-miR-125b), precursor (pre-miR-125b), and mature microRNA 125b (miR-125b) in vitro and in vivo. Methylation by NSun2 inhibits the processing of pri-miR-125b2 into pre-miR-125b2, decreases the cleavage of pre-miR-125b2 into miR-125, and attenuates the recruitment of RISC by miR-125, thereby repressing the function of miR-125b in silencing gene expression. Our results highlight the impact of miR-125b function via methylation by NSun2.

Entities: Chemical Gene Species

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Year: 2014 PMID： 25047833 PMCID： PMC4187725 DOI： 10.1128/MCB.00243-14

Source DB: PubMed Journal: Mol Cell Biol ISSN： 0270-7306 Impact factor: 4.272

36 in total

1. Redox regulation of Cdc25C.

Authors: Pavel A Savitsky; Toren Finkel
Journal: J Biol Chem Date: 2002-03-29 Impact factor: 5.157

2. Mouse Piwi-interacting RNAs are 2'-O-methylated at their 3' termini.

Authors: Yohei Kirino; Zissimos Mourelatos
Journal: Nat Struct Mol Biol Date: 2007-03-25 Impact factor: 15.369

3. Role of 16S ribosomal RNA methylations in translation initiation in Escherichia coli.

Authors: Gautam Das; Dinesh Kumar Thotala; Suman Kapoor; Sheelarani Karunanithi; Suman S Thakur; N Sadananda Singh; Umesh Varshney
Journal: EMBO J Date: 2008-02-21 Impact factor: 11.598

4. An in vitro system for accurate methylation of internal adenosine residues in messenger RNA.

Authors: P Narayan; F M Rottman
Journal: Science Date: 1988-11-25 Impact factor: 47.728

5. Context effects on N6-adenosine methylation sites in prolactin mRNA.

Authors: P Narayan; R L Ludwiczak; E C Goodwin; F M Rottman
Journal: Nucleic Acids Res Date: 1994-02-11 Impact factor: 16.971

6. The RNA methyltransferase Misu (NSun2) mediates Myc-induced proliferation and is upregulated in tumors.

Authors: Michaela Frye; Fiona M Watt
Journal: Curr Biol Date: 2006-05-23 Impact factor: 10.834

Review 7. The dual specificity phosphatase Cdc25C is a direct target for transcriptional repression by the tumor suppressor p53.

Authors: Selvon St Clair; James J Manfredi
Journal: Cell Cycle Date: 2006-04-01 Impact factor: 4.534

8. Pimet, the Drosophila homolog of HEN1, mediates 2'-O-methylation of Piwi- interacting RNAs at their 3' ends.

Authors: Kuniaki Saito; Yuriko Sakaguchi; Takeo Suzuki; Tsutomu Suzuki; Haruhiko Siomi; Mikiko C Siomi
Journal: Genes Dev Date: 2007-07-01 Impact factor: 11.361

9. The Drosophila RNA methyltransferase, DmHen1, modifies germline piRNAs and single-stranded siRNAs in RISC.

Authors: Michael D Horwich; Chengjian Li; Christian Matranga; Vasily Vagin; Gwen Farley; Peng Wang; Phillip D Zamore
Journal: Curr Biol Date: 2007-06-28 Impact factor: 10.834

10. Methylation as a crucial step in plant microRNA biogenesis.

Authors: Bin Yu; Zhiyong Yang; Junjie Li; Svetlana Minakhina; Maocheng Yang; Richard W Padgett; Ruth Steward; Xuemei Chen
Journal: Science Date: 2005-02-11 Impact factor: 47.728

38 in total

1. NSUN2-Mediated m5C Methylation and METTL3/METTL14-Mediated m6A Methylation Cooperatively Enhance p21 Translation.

Authors: Qiu Li; Xiu Li; Hao Tang; Bin Jiang; Yali Dou; Myriam Gorospe; Wengong Wang
Journal: J Cell Biochem Date: 2017-05-03 Impact factor: 4.429

2. NSun2 Promotes Cell Growth via Elevating Cyclin-Dependent Kinase 1 Translation.

Authors: Junyue Xing; Jie Yi; Xiaoyu Cai; Hao Tang; Zhenyun Liu; Xiaotian Zhang; Jennifer L Martindale; Xiaoling Yang; Bin Jiang; Myriam Gorospe; Wengong Wang
Journal: Mol Cell Biol Date: 2015-09-21 Impact factor: 4.272

3. Sequence-specific and Shape-selective RNA Recognition by the Human RNA 5-Methylcytosine Methyltransferase NSun6.

Authors: Tao Long; Jing Li; Hao Li; Mi Zhou; Xiao-Long Zhou; Ru-Juan Liu; En-Duo Wang
Journal: J Biol Chem Date: 2016-10-04 Impact factor: 5.157

4. Proteinase-activated receptor 2 promotes cancer cell migration through RNA methylation-mediated repression of miR-125b.

Authors: Lan Yang; Yiming Ma; Wenxiao Han; Weiwei Li; Liang Cui; Xinhua Zhao; Yantao Tian; Zhixiang Zhou; Wengong Wang; Hongying Wang
Journal: J Biol Chem Date: 2015-09-09 Impact factor: 5.157

Methylation by NSun2 represses the levels and function of microRNA 125b.

1. Redox regulation of Cdc25C.

2. Mouse Piwi-interacting RNAs are 2'-O-methylated at their 3' termini.

3. Role of 16S ribosomal RNA methylations in translation initiation in Escherichia coli.

4. An in vitro system for accurate methylation of internal adenosine residues in messenger RNA.

5. Context effects on N6-adenosine methylation sites in prolactin mRNA.

6. The RNA methyltransferase Misu (NSun2) mediates Myc-induced proliferation and is upregulated in tumors.

Review 7. The dual specificity phosphatase Cdc25C is a direct target for transcriptional repression by the tumor suppressor p53.

8. Pimet, the Drosophila homolog of HEN1, mediates 2'-O-methylation of Piwi- interacting RNAs at their 3' ends.

9. The Drosophila RNA methyltransferase, DmHen1, modifies germline piRNAs and single-stranded siRNAs in RISC.

10. Methylation as a crucial step in plant microRNA biogenesis.

1. NSUN2-Mediated m5C Methylation and METTL3/METTL14-Mediated m6A Methylation Cooperatively Enhance p21 Translation.

2. NSun2 Promotes Cell Growth via Elevating Cyclin-Dependent Kinase 1 Translation.

3. Sequence-specific and Shape-selective RNA Recognition by the Human RNA 5-Methylcytosine Methyltransferase NSun6.

4. Proteinase-activated receptor 2 promotes cancer cell migration through RNA methylation-mediated repression of miR-125b.

Review 5. Importance of m N⁶-methyladenosine (m⁶A) RNA modification in cancer.

6. Abnormal 5-methylcytosine lncRNA methylome is involved in human high-grade serous ovarian cancer.

7. miR-125/Pokemon auto-circuit contributes to the progression of hepatocellular carcinoma.

Review 8. Epigenetic regulation in chondrocyte phenotype maintenance for cell-based cartilage repair.

9. MicroRNAs Regulating Autophagy in Neurodegeneration.

Review 10. Sources and Functions of Extracellular Small RNAs in Human Circulation.

Review 7. The dual specificity phosphatase Cdc25C is a direct target for transcriptional repression by the tumor suppressor p53.

Review 5. Importance of m N6-methyladenosine (m6A) RNA modification in cancer.

Review 8. Epigenetic regulation in chondrocyte phenotype maintenance for cell-based cartilage repair.

Review 10. Sources and Functions of Extracellular Small RNAs in Human Circulation.

Review 5. Importance of m N⁶-methyladenosine (m⁶A) RNA modification in cancer.