Literature DB >> 28247949

NSUN2-Mediated m5C Methylation and METTL3/METTL14-Mediated m6A Methylation Cooperatively Enhance p21 Translation.

Qiu Li1, Xiu Li1, Hao Tang2, Bin Jiang1, Yali Dou3, Myriam Gorospe4, Wengong Wang1.   

Abstract

N6-methyladenosine (m6A) and m5C methylation are two major types of RNA methylation, but the impact of joint modifications on the same mRNA is unknown. Here, we show that in p21 3'UTR, NSUN2 catalyzes m5C modification and METTL3/METTL14 catalyzes m6A modification. Interestingly, methylation at m6A by METTL3/METTL14 facilitates the methylation of m5C by NSUN2, and vice versa. NSUN2-mediated m5C and METTL3/METTL14-mediated m6A methylation synergistically enhance p21 expression at the translational level, leading to elevated expression of p21 in oxidative stress-induced cellular senescence. Our findings on p21 mRNA methylation and expression reveal that joint m6A and m5C modification of the same RNA may influence each other, coordinately affecting protein expression patterns. J. Cell. Biochem. 118: 2587-2598, 2017.
© 2017 Wiley Periodicals, Inc. © 2017 Wiley Periodicals, Inc.

Entities:  

Keywords:  METTL14; METTL3; NSUN2; TRANSLATIONAL REGULATION; p21 mRNA METHYLATION

Mesh：

Substances：

Year:  2017        PMID： 28247949      PMCID： PMC5509477          DOI： 10.1002/jcb.25957

Source DB:  PubMed          Journal:  J Cell Biochem        ISSN： 0730-2312            Impact factor:   4.429


  36 in total

1.  Transcriptome-wide mapping reveals reversible and dynamic N(1)-methyladenosine methylome.

Authors:  Xiaoyu Li; Xushen Xiong; Kun Wang; Lixia Wang; Xiaoting Shu; Shiqing Ma; Chengqi Yi
Journal:  Nat Chem Biol       Date:  2016-02-10       Impact factor: 15.040

2.  Simultaneous Quantification of Methylated Cytidine and Adenosine in Cellular and Tissue RNA by Nano-Flow Liquid Chromatography-Tandem Mass Spectrometry Coupled with the Stable Isotope-Dilution Method.

Authors:  Lijuan Fu; Nicholas J Amato; Pengcheng Wang; Sara J McGowan; Laura J Niedernhofer; Yinsheng Wang
Journal:  Anal Chem       Date:  2015-07-21       Impact factor: 6.986

Review 3.  Transfer RNA modification.

Authors:  G R Björk; J U Ericson; C E Gustafsson; T G Hagervall; Y H Jönsson; P M Wikström
Journal:  Annu Rev Biochem       Date:  1987       Impact factor: 23.643

4.  Comprehensive analysis of mRNA methylation reveals enrichment in 3' UTRs and near stop codons.

Authors:  Kate D Meyer; Yogesh Saletore; Paul Zumbo; Olivier Elemento; Christopher E Mason; Samie R Jaffrey
Journal:  Cell       Date:  2012-05-17       Impact factor: 41.582

5.  Protective role of p21(Waf1/Cip1) against prostaglandin A2-mediated apoptosis of human colorectal carcinoma cells.

Authors:  M Gorospe; X Wang; K Z Guyton; N J Holbrook
Journal:  Mol Cell Biol       Date:  1996-12       Impact factor: 4.272

6.  The methylation state of poly A-containing messenger RNA from cultured hamster cells.

Authors:  D T Dubin; R H Taylor
Journal:  Nucleic Acids Res       Date:  1975-10       Impact factor: 16.971

Review 7.  Transcriptional regulation of the p21((WAF1/CIP1)) gene.

Authors:  A L Gartel; A L Tyner
Journal:  Exp Cell Res       Date:  1999-02-01       Impact factor: 3.905

8.  Role of p21 in prostaglandin A2-mediated cellular arrest and death.

Authors:  M Gorospe; N J Holbrook
Journal:  Cancer Res       Date:  1996-02-01       Impact factor: 12.701

9.  NSun2 delays replicative senescence by repressing p27 (KIP1) translation and elevating CDK1 translation.

Authors:  Hao Tang; Xiuqin Fan; Junyue Xing; Zhenyun Liu; Bin Jiang; Yali Dou; Myriam Gorospe; Wengong Wang
Journal:  Aging (Albany NY)       Date:  2015-12       Impact factor: 5.682

10.  NSun2-mediated cytosine-5 methylation of vault noncoding RNA determines its processing into regulatory small RNAs.

Authors:  Shobbir Hussain; Abdulrahim A Sajini; Sandra Blanco; Sabine Dietmann; Patrick Lombard; Yoichiro Sugimoto; Maike Paramor; Joseph G Gleeson; Duncan T Odom; Jernej Ule; Michaela Frye
Journal:  Cell Rep       Date:  2013-07-18       Impact factor: 9.423
View more
  71 in total

1.  Epitranscriptomic Addition of m5C to HIV-1 Transcripts Regulates Viral Gene Expression.

Authors:  David G Courtney; Kevin Tsai; Hal P Bogerd; Edward M Kennedy; Brittany A Law; Ann Emery; Ronald Swanstrom; Christopher L Holley; Bryan R Cullen
Journal:  Cell Host Microbe       Date:  2019-08-14       Impact factor: 21.023

2.  RNA bisulfite sequencing reveals NSUN2-mediated suppression of epithelial differentiation in pancreatic cancer.

Authors:  Szu-Ying Chen; Kuan-Lin Chen; Li-Yun Ding; Chien-Hung Yu; Hsin-Yi Wu; Ya-Yi Chou; Chia-Jung Chang; Chih-Han Chang; Ya-Na Wu; Shang-Rung Wu; Ya-Chin Hou; Chung-Ta Lee; Peng-Chieh Chen; Yan-Shen Shan; Po-Hsien Huang
Journal:  Oncogene       Date:  2022-05-02       Impact factor: 9.867

Review 3.  How RNA modifications regulate the antiviral response.

Authors:  Matthew G Thompson; Matthew T Sacco; Stacy M Horner
Journal:  Immunol Rev       Date:  2021-08-17       Impact factor: 12.988

Review 4.  Epitranscriptomics in the Heart: a Focus on m6A.

Authors:  Jacob Z Longenecker; Christopher J Gilbert; Volha A Golubeva; Colton R Martens; Federica Accornero
Journal:  Curr Heart Fail Rep       Date:  2020-10

5.  Dynamic methylome of internal mRNA N7-methylguanosine and its regulatory role in translation.

Authors:  Lionel Malbec; Ting Zhang; Yu-Sheng Chen; Ying Zhang; Bao-Fa Sun; Bo-Yang Shi; Yong-Liang Zhao; Ying Yang; Yun-Gui Yang
Journal:  Cell Res       Date:  2019-09-13       Impact factor: 25.617

Review 6.  Epitranscriptomic Modifications Modulate Normal and Pathological Functions in CNS.

Authors:  Anil K Chokkalla; Suresh L Mehta; Raghu Vemuganti
Journal:  Transl Stroke Res       Date:  2021-07-05       Impact factor: 6.829

Review 7.  Epigenetic regulations in mammalian spermatogenesis: RNA-m6A modification and beyond.

Authors:  Yiqian Gui; Shuiqiao Yuan
Journal:  Cell Mol Life Sci       Date:  2021-04-09       Impact factor: 9.261

8.  Involvement of METTL3/m6Adenosine and TGFβ/Smad3 signaling on Tenon's fibroblasts and in a rabbit model of glaucoma surgery.

Authors:  Yan Liu; Chao Gu; Xiabin Li; Tingwei Wang; Ling Yu
Journal:  J Mol Histol       Date:  2021-11-02       Impact factor: 2.611

Review 9.  RNA Methylation in Systemic Lupus Erythematosus.

Authors:  Xinyi Lv; Xiaomin Liu; Ming Zhao; Haijing Wu; Wuiguang Zhang; Qianjin Lu; Xiangmei Chen
Journal:  Front Cell Dev Biol       Date:  2021-07-07

Review 10.  Synthetic modified messenger RNA for therapeutic applications.

Authors:  Minsong Gao; Qingyi Zhang; Xin-Hua Feng; Jianzhao Liu
Journal:  Acta Biomater       Date:  2021-06-13       Impact factor: 8.947
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.