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Literature DB >> 2469000

Selection and characterization of ricin toxin A-chain mutations in Saccharomyces cerevisiae.

A Frankel¹, D Schlossman, P Welsh, A Hertler, D Withers, S Johnston.

Abstract

A DNA sequence encoding the A chain of ricin toxin (RTA) from the castor bean plant, Ricinus communis, was placed under GAL1 promoter control and transformed into Saccharomyces cerevisiae. Induction of expression of RTA was lethal. This lethality was the basis for a selection of mutations in RTA which inactivated the toxin. A number of mutant alleles which encoded cross-reactive material were sequenced. Eight of the first nine mutant RTAs studied showed single-amino-acid changes involving residues located in the proposed active-site cleft.

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Year: 1989 PMID： 2469000 PMCID： PMC362616 DOI： 10.1128/mcb.9.2.415-420.1989

Source DB: PubMed Journal: Mol Cell Biol ISSN： 0270-7306 Impact factor: 4.272

21 in total

1. Conditional mutator gene in Escherichia coli: isolation, mapping, and effector studies.

Authors: G E Degnen; E C Cox
Journal: J Bacteriol Date: 1974-02 Impact factor: 3.490

2. Treatment of abrin and ricin with -mercaptoethanol opposite effects on their toxicity in mice and their ability to inhibit protein synthesis in a cell-free system.

Authors: S Olsnes; A Pihl
Journal: FEBS Lett Date: 1972-11-15 Impact factor: 4.124

3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

4. A rapid boiling method for the preparation of bacterial plasmids.

Authors: D S Holmes; M Quigley
Journal: Anal Biochem Date: 1981-06 Impact factor: 3.365

5. Entry of lethal doses of abrin, ricin and modeccin into the cytosol of HeLa cells.

Authors: K Eiklid; S Olsnes; A Pihl
Journal: Exp Cell Res Date: 1980-04 Impact factor: 3.905

6. Expression of ricin A chain in Escherichia coli.

Authors: M O'Hare; L M Roberts; P E Thorpe; G J Watson; B Prior; J M Lord
Journal: FEBS Lett Date: 1987-05-25 Impact factor: 4.124

7. Construction, replication, and chromatin structure of TRP1 RI circle, a multiple-copy synthetic plasmid derived from Saccharomyces cerevisiae chromosomal DNA.

Authors: V A Zakian; J F Scott
Journal: Mol Cell Biol Date: 1982-03 Impact factor: 4.272

8. DNA sequencing with chain-terminating inhibitors.

Authors: F Sanger; S Nicklen; A R Coulson
Journal: Proc Natl Acad Sci U S A Date: 1977-12 Impact factor: 11.205

9. RNA from the yeast transposable element Ty1 has both ends in the direct repeats, a structure similar to retrovirus RNA.

Authors: R T Elder; E Y Loh; R W Davis
Journal: Proc Natl Acad Sci U S A Date: 1983-05 Impact factor: 11.205

10. Isolation and characterization of a novel ribosome-inactivating protein from root cultures of pokeweed and its mechanism of secretion from roots.

Authors: Sang-Wook Park; Christopher B Lawrence; James C Linden; Jorge M Vivanco
Journal: Plant Physiol Date: 2002-09 Impact factor: 8.340

Selection and characterization of ricin toxin A-chain mutations in Saccharomyces cerevisiae.

1. Conditional mutator gene in Escherichia coli: isolation, mapping, and effector studies.

2. Treatment of abrin and ricin with -mercaptoethanol opposite effects on their toxicity in mice and their ability to inhibit protein synthesis in a cell-free system.

3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

4. A rapid boiling method for the preparation of bacterial plasmids.

5. Entry of lethal doses of abrin, ricin and modeccin into the cytosol of HeLa cells.

6. Expression of ricin A chain in Escherichia coli.

7. Construction, replication, and chromatin structure of TRP1 RI circle, a multiple-copy synthetic plasmid derived from Saccharomyces cerevisiae chromosomal DNA.

8. DNA sequencing with chain-terminating inhibitors.

9. RNA from the yeast transposable element Ty1 has both ends in the direct repeats, a structure similar to retrovirus RNA.

10. Transformation of intact yeast cells treated with alkali cations.

1. Exploring steric constraints on protein mutations using MAGE/PROBE.

2. Cloning and characterisation of a gene encoding an antiviral protein from Clerodendrum aculeatum L.

3. Role of glutamic acid 177 of the ricin toxin A chain in enzymatic inactivation of ribosomes.

4. Determination by systematic deletion of the amino acids essential for catalysis by ricin A chain.

5. A maize ribosome-inactivating protein is controlled by the transcriptional activator Opaque-2.

6. Role of arginine 180 and glutamic acid 177 of ricin toxin A chain in enzymatic inactivation of ribosomes.

7. Dislocation of ricin toxin A chains in human cells utilizes selective cellular factors.

8. Mutational analysis of the Shiga toxin and Shiga-like toxin II enzymatic subunits.

9. Intracellular expression of toxic shock syndrome toxin 1 in Saccharomyces cerevisiae.

10. Isolation and characterization of a novel ribosome-inactivating protein from root cultures of pokeweed and its mechanism of secretion from roots.