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Literature DB >> 2689871

Role of glutamic acid 177 of the ricin toxin A chain in enzymatic inactivation of ribosomes.

D Schlossman¹, D Withers, P Welsh, A Alexander, J Robertus, A Frankel.

Abstract

The gene for the A chain of ricin toxin was fused to a beta-galactosidase marker cistron via a DNA sequence encoding a short collagen linker, and the tripartite fusion protein was expressed in Escherichia coli. Site-specific mutagenesis was used to change glutamic acid residue 177 to aspartic acid or alanine. When the mutant proteins were expressed, purified, and tested quantitatively for enzymatic activity, the carboxylate function at position 177 was found not to be absolutely essential for ricin toxin A-chain catalysis.

Entities: Chemical Mutation Species

Mesh：

Substances：

Year: 1989 PMID： 2689871 PMCID： PMC363653 DOI： 10.1128/mcb.9.11.5012-5021.1989

Source DB: PubMed Journal: Mol Cell Biol ISSN： 0270-7306 Impact factor: 4.272

29 in total

1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

2. Rapid purification of a cloned gene product by genetic fusion and site-specific proteolysis.

Authors: J Germino; D Bastia
Journal: Proc Natl Acad Sci U S A Date: 1984-08 Impact factor: 11.205

3. A rapid boiling method for the preparation of bacterial plasmids.

Authors: D S Holmes; M Quigley
Journal: Anal Biochem Date: 1981-06 Impact factor: 3.365

4. Use of gene fusions and protein-protein interaction in the isolation of a biologically active regulatory protein: the replication initiator protein of plasmid R6K.

Authors: J Germino; J G Gray; H Charbonneau; T Vanaman; D Bastia
Journal: Proc Natl Acad Sci U S A Date: 1983-11 Impact factor: 11.205

5. Routing of internalized ricin and ricin conjugates to the Golgi complex.

Authors: B van Deurs; T I Tønnessen; O W Petersen; K Sandvig; S Olsnes
Journal: J Cell Biol Date: 1986-01 Impact factor: 10.539

6. Induction of galactokinase in Saccharomyces cerevisiae: kinetics of induction and glucose effects.

Authors: B G Adams
Journal: J Bacteriol Date: 1972-08 Impact factor: 3.490

7. Involvement of arginine residues in inhibition of protein synthesis by ricin A-chain.

Authors: K Watanabe; G Funatsu
Journal: FEBS Lett Date: 1986-08-18 Impact factor: 4.124

8. Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae.

Authors: M Johnston; R W Davis
Journal: Mol Cell Biol Date: 1984-08 Impact factor: 4.272

9. Genomic cloning and characterization of a ricin gene from Ricinus communis.

Authors: K C Halling; A C Halling; E E Murray; B F Ladin; L L Houston; R F Weaver
Journal: Nucleic Acids Res Date: 1985-11-25 Impact factor: 16.971

10. Ribosome inactivation by ricin A chain: a sensitive method to assess the activity of wild-type and mutant polypeptides.

Authors: M J May; M R Hartley; L M Roberts; P A Krieg; R W Osborn; J M Lord
Journal: EMBO J Date: 1989-01 Impact factor: 11.598

25 in total

8. Modeling of the three-dimensional structure of luffin-alpha and its simulated reaction with the substrate oligoribonucleotide GAGA.

Authors: R S Chen; H W Leung; Y C Dong; R N Wong
Journal: J Protein Chem Date: 1996-10

9. Investigation of ribosome binding by the Shiga toxin A1 subunit, using competition and site-directed mutagenesis.

Authors: L M Skinner; M P Jackson
Journal: J Bacteriol Date: 1997-02 Impact factor: 3.490

Review 10. Targeting ricin to the ribosome.

Authors: Kerrie L May; Qing Yan; Nilgun E Tumer
Journal: Toxicon Date: 2013-02-20 Impact factor: 3.033

Role of glutamic acid 177 of the ricin toxin A chain in enzymatic inactivation of ribosomes.

1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

2. Rapid purification of a cloned gene product by genetic fusion and site-specific proteolysis.

3. A rapid boiling method for the preparation of bacterial plasmids.

4. Use of gene fusions and protein-protein interaction in the isolation of a biologically active regulatory protein: the replication initiator protein of plasmid R6K.

5. Routing of internalized ricin and ricin conjugates to the Golgi complex.

6. Induction of galactokinase in Saccharomyces cerevisiae: kinetics of induction and glucose effects.

7. Involvement of arginine residues in inhibition of protein synthesis by ricin A-chain.

8. Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae.

9. Genomic cloning and characterization of a ricin gene from Ricinus communis.

10. Ribosome inactivation by ricin A chain: a sensitive method to assess the activity of wild-type and mutant polypeptides.

1. Exploring steric constraints on protein mutations using MAGE/PROBE.

2. Improved stability of a protein vaccine through elimination of a partially unfolded state.

3. Identification of amino acids critical for the cytotoxicity of Shiga toxin 1 and 2 in Saccharomyces cerevisiae.

4. Determination by systematic deletion of the amino acids essential for catalysis by ricin A chain.

5. Identification of small-molecule inhibitors of ricin and shiga toxin using a cell-based high-throughput screen.

6. Role of arginine 180 and glutamic acid 177 of ricin toxin A chain in enzymatic inactivation of ribosomes.

7. Dislocation of ricin toxin A chains in human cells utilizes selective cellular factors.

8. Modeling of the three-dimensional structure of luffin-alpha and its simulated reaction with the substrate oligoribonucleotide GAGA.

9. Investigation of ribosome binding by the Shiga toxin A1 subunit, using competition and site-directed mutagenesis.

Review 10. Targeting ricin to the ribosome.