| Literature DB >> 3556218 |
M O'Hare, L M Roberts, P E Thorpe, G J Watson, B Prior, J M Lord.
Abstract
DNA encoding ricin A chain was derived from preproricin cDNA and ligated into the expression vector pDS5/3. Transcription is controlled from the coliphage promoter PN25 fused with the lac operator of E.coli. When induced, E.coli 71.18 cells transformed with the recombinant plasmid express ricin A chain which is soluble and has full biological activity.Entities:
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Year: 1987 PMID： 3556218 DOI： 10.1016/0014-5793(87)80759-7
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124