Resolving directional ambiguity in dynamic light scattering-based transverse motion velocimetry in optical coherence tomography.

Dynamic light scattering-based optical coherence tomography approaches have been successfully implemented to measure total transverse (xy) flow speed, but are unable to resolve directionality. We propose a method to extract directional velocity in the transverse plane by introducing a variable scan bias to our system. Our velocity estimation, which yields the directional velocity component along the scan axis, is also independent of any point-spread function calibration. By combining our approach with Doppler velocimetry, we show three-component velocimetry that is appropriately dependent on latitudinal and longitudinal angle.