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Literature DB >> 24248764

Transformation of sugarcane protoplasts by direct uptake of a selectable chimaeric gene.

W H Chen¹, K M Gartland, M R Davey, R Sotak, J S Gartland, B J Mulligan, J B Power, E C Cocking.

Abstract

Sugarcane protoplasts were transformed to kanamycin resistance at a frequency of approximately 8 in 10(7) following PEG-induced uptake of Sma1 linearised pABD1 plasmid. DNA-treated protoplasts were cultured in agarose droplets, and protoplast-derived transformants selected on 80 μg ml(-1) kanamycin. Transformed tissues maintained on this level of antibiotic expressed APH(3')II activity, and contained DNA that hybridised to a probe with the APH(3')II gene.

Entities: Chemical Species

Year: 1987 PMID： 24248764 DOI： 10.1007/BF00272003

Source DB: PubMed Journal: Plant Cell Rep ISSN： 0721-7714 Impact factor: 4.570

15 in total

1. Transformation of plant protoplasts with DNA: cotransformation of non-selected calf thymus carrier DNA and meiotic segregation of transforming DNA sequences.

Authors: R Peerbolte; F A Krens; R M Mans; M Floor; J H Hoge; G J Wullems; R A Schilperoort
Journal: Plant Mol Biol Date: 1985-07 Impact factor: 4.076

2. Stable transformation of maize after gene transfer by electroporation.

Authors: M E Fromm; L P Taylor; V Walbot
Journal: Nature Date: 1986 Feb 27-Mar 5 Impact factor: 49.962

3. Modifications of the diphenylamine reaction giving increased sensitivity and simplicity in the estimation of DNA.

Authors: G M Richards
Journal: Anal Biochem Date: 1974-02 Impact factor: 3.365

4. The isolation, culture and regeneration of Petunia leaf protoplasts.

Authors: E M Frearson; J B Power; E C Cocking
Journal: Dev Biol Date: 1973-07 Impact factor: 3.582

5. A new sensitive method for qualitative and quantitative assay of neomycin phosphotransferase in crude cell extracts.

Authors: B Reiss; R Sprengel; H Will; H Schaller
Journal: Gene Date: 1984-10 Impact factor: 3.688

6. Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure.

Authors: F C Kafatos; C W Jones; A Efstratiadis
Journal: Nucleic Acids Res Date: 1979-11-24 Impact factor: 16.971

7. Chimeric vector construction for higher-plant transformation.

Authors: E Balázs; S Bouzoubaa; H Guilley; G Jonard; J Paszkowski; K Richards
Journal: Gene Date: 1985 Impact factor: 3.688

8. The transformation of Zea mays seedlings with Agrobacterium tumefaciens.

Authors: A C Graves; S L Goldman
Journal: Plant Mol Biol Date: 1986-01 Impact factor: 4.076

9. Genetic transformation of Brassica campestris var. rapa protoplasts with an engineered cauliflower mosaic virus genome.

Authors: J Paszkowski; B Pisan; R D Shillito; T Hohn; B Hohn; I Potrykus
Journal: Plant Mol Biol Date: 1986-09 Impact factor: 4.076

10. An Agrobacterium-transformed cell culture from the monocot Asparagus officinalis.

Authors: J P Hernalsteens; L Thia-Toong; J Schell; M Van Montagu
Journal: EMBO J Date: 1984-12-20 Impact factor: 11.598

13 in total

1. Transgenic rice plants produced by electroporation-mediated plasmid uptake into protoplasts.

Authors: H M Zhang; H Yang; E L Rech; T J Golds; A S Davis; B J Mulligan; E C Cocking; M R Davey
Journal: Plant Cell Rep Date: 1988-10 Impact factor: 4.570

2. Stably transformed herbicide resistant callus of sugarcane via microprojectile bombardment of cell suspension cultures and electroporation of protoplasts.

Authors: M K Chowdhury; I K Vasil
Journal: Plant Cell Rep Date: 1992-09 Impact factor: 4.570

3. Microprojectile transformation of sugarcane meristems and regeneration of shoots expressing β-Glucuronidase.

Authors: R L Gambley; R Ford; G R Smith
Journal: Plant Cell Rep Date: 1993-04 Impact factor: 4.570

4. Effects of tissue type and promoter strength on transient GUS expression in sugarcane following particle bombardment.

Authors: M Gallo-Meagher; J E Irvine
Journal: Plant Cell Rep Date: 1993-10 Impact factor: 4.570

Review 5. Direct DNA transfer to plant cells.

Authors: M R Davey; E L Rech; B J Mulligan
Journal: Plant Mol Biol Date: 1989-09 Impact factor: 4.076

6. Efficient transformation of Arabidopsis thaliana using direct gene transfer to protoplasts.

Authors: B Damm; R Schmidt; L Willmitzer
Journal: Mol Gen Genet Date: 1989-05

7. Introduction and differential use of various promoters in pollen grains of Nicotiana glutinosa and Lilium longiflorum.

Authors: L M van der Leede-Plegt; B C van de Ven; R J Bino; T P van der Salm; A J van Tunen
Journal: Plant Cell Rep Date: 1992-02 Impact factor: 4.570

8. Factors affecting PEG-mediated stable transformation of maize protoplasts.

Authors: C L Armstrong; W L Petersen; W G Buchholz; B A Bowen; S L Sulc
Journal: Plant Cell Rep Date: 1990-10 Impact factor: 4.570

9. Production of transgenic sugarcane (Saccharum officinarum L.) plants by intact cell electroporation.

Authors: A Arencibia; P R Molina; G de la Riva; G Selman-Housein
Journal: Plant Cell Rep Date: 1995-02 Impact factor: 4.570

10. Comparative analysis of free DNA delivery and expression into protoplasts of Panicum maximum Jacq. (Guinea grass) by electroporation and polyethylene glycol.

Authors: V Vasil; R M Hauptmann; F M Morrish; I K Vasil
Journal: Plant Cell Rep Date: 1988-12 Impact factor: 4.570