Literature DB >> 24307380

Genetic transformation of Brassica campestris var. rapa protoplasts with an engineered cauliflower mosaic virus genome.

J Paszkowski1, B Pisan, R D Shillito, T Hohn, B Hohn, I Potrykus.   

Abstract

A hybrid Cauliflower Mosaic Virus (CaMV) genome containing a selectable marker gene was constructed by replacing the gene VI coding region with the aminoglycoside (neomycin) phosphotransferase type II [APH(3')II] gene from Tn5. This modified viral genome was tested for its infectivity both in planta and in a protoplast transformation system of Brassica campestris var. rapa. Stable, genetically transformed cell lines of B. campestris var. rapa were obtained after transformation. DNA of the hybrid CaMV genome was found to be integrated into high molecular weight plant genomic DNA. Transformation was achieved only when the hybrid genome was supplied together with wild type viral DNA. A possible complementation of the modified CaMV genome with the wild type viral DNA as a helper molecule in planta and in the protoplast system is discussed.

Entities:  

Year:  1986        PMID： 24307380     DOI： 10.1007/BF00034937

Source DB:  PubMed          Journal:  Plant Mol Biol        ISSN： 0167-4412            Impact factor:   4.076


  27 in total

1.  Phaseolin gene from bean is expressed after transfer to sunflower via tumor-inducing plasmid vectors.

Authors:  N Murai; J D Kemp; D W Sutton; M G Murray; J L Slightom; D J Merlo; N A Reichert; C Sengupta-Gopalan; C A Stock; R F Barker; T C Hall
Journal:  Science       Date:  1983-11-04       Impact factor: 47.728

2.  Incorporation and maintenance of recombinant-DNA plasmid vehicles pBR313 and pCR1 in plant protoplasts.

Authors:  S M Fernandez; P F Lurquin; C I Kado
Journal:  FEBS Lett       Date:  1978-03-15       Impact factor: 4.124

3.  Expression of bacterial genes in plant cells.

Authors:  R T Fraley; S G Rogers; R B Horsch; P R Sanders; J S Flick; S P Adams; M L Bittner; L A Brand; C L Fink; J S Fry; G R Galluppi; S B Goldberg; N L Hoffmann; S C Woo
Journal:  Proc Natl Acad Sci U S A       Date:  1983-08       Impact factor: 11.205

4.  Somatic embryogenesis in quite a direct way in cultures of mesophyll protoplasts of Brassica napus L.

Authors:  L C Li; H W Kohlenbach
Journal:  Plant Cell Rep       Date:  1982-10       Impact factor: 4.570

5.  In vivo recombination of cauliflower mosaic virus DNA.

Authors:  G Lebeurier; L Hirth; B Hohn; T Hohn
Journal:  Proc Natl Acad Sci U S A       Date:  1982-05       Impact factor: 11.205

Review 6.  T-DNA of the Agrobacterium Ti and Ri plasmids.

Authors:  M W Bevan; M D Chilton
Journal:  Annu Rev Genet       Date:  1982       Impact factor: 16.830

7.  Expression of a transposable antibiotic resistance element in Saccharomyces.

Authors:  A Jimenez; J Davies
Journal:  Nature       Date:  1980-10-30       Impact factor: 49.962

8.  Involvement of reverse transcription in the replication of cauliflower mosaic virus: a detailed model and test of some aspects.

Authors:  P Pfeiffer; T Hohn
Journal:  Cell       Date:  1983-07       Impact factor: 41.582

9.  Chimeric genes as dominant selectable markers in plant cells.

Authors:  L Herrera-Estrella; M D Block; E Messens; J P Hernalsteens; M V Montagu; J Schell
Journal:  EMBO J       Date:  1983       Impact factor: 11.598

10.  Identification by immunoprecipitation of cauliflower mosaic virus in vitro major translation product with a specific serum against viroplasm protein.

Authors:  C Xiong; S Muller; G Lebeurier; L Hirth
Journal:  EMBO J       Date:  1982       Impact factor: 11.598
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  8 in total

1.  Transformation of cauliflower (Brassica oleracea L. var. botrytis)--an experimental survey.

Authors:  K Eimert; F Siegemund
Journal:  Plant Mol Biol       Date:  1992-06       Impact factor: 4.076

2.  Transgenic rice plants produced by electroporation-mediated plasmid uptake into protoplasts.

Authors:  H M Zhang; H Yang; E L Rech; T J Golds; A S Davis; B J Mulligan; E C Cocking; M R Davey
Journal:  Plant Cell Rep       Date:  1988-10       Impact factor: 4.570

3.  Transfer of hygromycin resistance into Brassica napus using total DNA of a transgenic B. nigra line.

Authors:  C Golz; F Köhler; O Schieder
Journal:  Plant Mol Biol       Date:  1990-09       Impact factor: 4.076

Review 4.  Direct DNA transfer to plant cells.

Authors:  M R Davey; E L Rech; B J Mulligan
Journal:  Plant Mol Biol       Date:  1989-09       Impact factor: 4.076

5.  Efficient transformation of Arabidopsis thaliana using direct gene transfer to protoplasts.

Authors:  B Damm; R Schmidt; L Willmitzer
Journal:  Mol Gen Genet       Date:  1989-05

6.  Transformation of sugarcane protoplasts by direct uptake of a selectable chimaeric gene.

Authors:  W H Chen; K M Gartland; M R Davey; R Sotak; J S Gartland; B J Mulligan; J B Power; E C Cocking
Journal:  Plant Cell Rep       Date:  1987-07       Impact factor: 4.570

7.  Stable transformation of moth bean Vigna aconitifolia via direct gene transfer.

Authors:  F Köhler; C Golz; S Eapen; H Kohn; O Schieder
Journal:  Plant Cell Rep       Date:  1987-07       Impact factor: 4.570

8.  Production of kanamycin resistant rice tissues following DNA uptake into protoplasts.

Authors:  H Yang; H M Zhang; M R Davey; B J Mulligan; E C Cocking
Journal:  Plant Cell Rep       Date:  1988-10       Impact factor: 4.570
  8 in total

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