Literature DB >> 24197261

Microprojectile transformation of sugarcane meristems and regeneration of shoots expressing β-Glucuronidase.

R L Gambley1, R Ford, G R Smith.   

Abstract

Microprojectile bombardment was used to introduce the GUS reporter gene into sugarcane axillary meristems. Chimeric expression of this gene was observed in 20-40% of shoots regenerated from sugarcane meristems one month after particle bombardment. The linear pattern of GUS expression observed is consistent with periclinal division from single transformed meristematic cells. Meristems have advantages over callus cells as targets for microprojectile transformation, and have potential for introducing agronomically important genes into current commercial sugarcane varieties.

Entities:  

Year:  1993        PMID： 24197261     DOI： 10.1007/BF00237432

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  8 in total

1.  Transient expression of the coat protein of sugarcane mosaic virus in sugarcane protoplasts and expression in Escherichia coli.

Authors:  G R Smith; R Ford; M J Frenkel; D D Shukla; J L Dale
Journal:  Arch Virol       Date:  1992       Impact factor: 2.574

2.  Stably transformed herbicide resistant callus of sugarcane via microprojectile bombardment of cell suspension cultures and electroporation of protoplasts.

Authors:  M K Chowdhury; I K Vasil
Journal:  Plant Cell Rep       Date:  1992-09       Impact factor: 4.570

3.  pEmu: an improved promoter for gene expression in cereal cells.

Authors:  D I Last; R I Brettell; D A Chamberlain; A M Chaudhury; P J Larkin; E L Marsh; W J Peacock; E S Dennis
Journal:  Theor Appl Genet       Date:  1991-05       Impact factor: 5.699

4.  Endophytic bacteria expressing β-glucuronidase cause false positives in transformation of Dioscorea species.

Authors:  M Tör; S H Mantell; C Ainsworth
Journal:  Plant Cell Rep       Date:  1992-08       Impact factor: 4.570

5.  Transgenic rice cell lines and plants: expression of transferred chimeric genes.

Authors:  E G Meijer; R A Schilperoort; S Rueb; P E van Os-Ruygrok; L A Hensgens
Journal:  Plant Mol Biol       Date:  1991-05       Impact factor: 4.076

6.  beta-Glucuronidase from Escherichia coli as a gene-fusion marker.

Authors:  R A Jefferson; S M Burgess; D Hirsh
Journal:  Proc Natl Acad Sci U S A       Date:  1986-11       Impact factor: 11.205

7.  Histology of, and physical factors affecting, transient GUS expression in pearl millet (Pennisetum glaucum (L.) R. Br.) embryos following microprojectile bombardment.

Authors:  M G Taylor; I K Vasil
Journal:  Plant Cell Rep       Date:  1991-06       Impact factor: 4.570

8.  Transformation of sugarcane protoplasts by direct uptake of a selectable chimaeric gene.

Authors:  W H Chen; K M Gartland; M R Davey; R Sotak; J S Gartland; B J Mulligan; J B Power; E C Cocking
Journal:  Plant Cell Rep       Date:  1987-07       Impact factor: 4.570
  8 in total
  3 in total

1.  Refining the application of direct embryogenesis in sugarcane: Effect of the developmental phase of leaf disc explants and the timing of DNA transfer on transformation efficiency.

Authors:  S J Snyman; G M Meyer; J M Richards; N Haricharan; S Ramgareeb; B I Huckett
Journal:  Plant Cell Rep       Date:  2006-03-28       Impact factor: 4.570

2.  Effects of tissue type and promoter strength on transient GUS expression in sugarcane following particle bombardment.

Authors:  M Gallo-Meagher; J E Irvine
Journal:  Plant Cell Rep       Date:  1993-10       Impact factor: 4.570

3.  Expression, purification, and use as an antigen of recombinant sugarcane mosaic virus coat protein.

Authors:  G R Smith; R Ford; J D Bryant; R L Gambley; T K McGhie; R M Harding; J L Dale
Journal:  Arch Virol       Date:  1995       Impact factor: 2.574
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.