Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Comparative analysis of free DNA delivery and expression into protoplasts of Panicum maximum Jacq. (Guinea grass) by electroporation and polyethylene glycol.

Literature DB >> 24240401

Comparative analysis of free DNA delivery and expression into protoplasts of Panicum maximum Jacq. (Guinea grass) by electroporation and polyethylene glycol.

V Vasil¹, R M Hauptmann, F M Morrish, I K Vasil.

Abstract

Relative levels of gene expression were studied in protoplasts isolated from two cell lines of Panicum maximum following DNA delivery by electroporation and polyethylene glycol (PEG). Gene expression was evaluated by assaying for chloramphenicol acetyltransferase (CAT) activity expressed by the CaMV 35S promoter with a nopaline synthase 3' polyadenylation signal, approximately 48 hours after DNA delivery. The expression of the CAT gene was slightly higher in electroporated protoplasts in comparison to PEG mediated delivery. However, PEG treated protoplasts showed higher plating efficiency. The effect of different salts and the molecular weight of PEG used on gene expression was also studied.

Entities: Chemical Species

Year: 1988 PMID： 24240401 DOI： 10.1007/BF00272741

Source DB: PubMed Journal: Plant Cell Rep ISSN： 0721-7714 Impact factor: 4.570

11 in total

1. Expression of a foreign gene linked to either a plant-virus or a Drosophila promoter, after electroporation of protoplasts of rice, wheat, and sorghum.

Authors: T M Ou-Lee; R Turgeon; R Wu
Journal: Proc Natl Acad Sci U S A Date: 1986-09 Impact factor: 11.205

2. Stable transformation of maize after gene transfer by electroporation.

Authors: M E Fromm; L P Taylor; V Walbot
Journal: Nature Date: 1986 Feb 27-Mar 5 Impact factor: 49.962

3. Expression of genes transferred into monocot and dicot plant cells by electroporation.

Authors: M Fromm; L P Taylor; V Walbot
Journal: Proc Natl Acad Sci U S A Date: 1985-09 Impact factor: 11.205

4. Genetically transformed maize plants from protoplasts.

Authors: C A Rhodes; D A Pierce; I J Mettler; D Mascarenhas; J J Detmer
Journal: Science Date: 1988-04-08 Impact factor: 47.728

5. Evaluation of selectable markers for obtaining stable transformants in the gramineae.

Authors: R M Hauptmann; V Vasil; P Ozias-Akins; Z Tabaeizadeh; S G Rogers; R T Fraley; R B Horsch; I K Vasil
Journal: Plant Physiol Date: 1988-02 Impact factor: 8.340

6. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.

Authors: C M Gorman; L F Moffat; B H Howard
Journal: Mol Cell Biol Date: 1982-09 Impact factor: 4.272

7. Isolation and culture of cereal protoplasts : Part 2: Embryogenesis and plantlet formation from protoplasts of Pennisetum americanum.

Authors: V Vasil; I K Vasil
Journal: Theor Appl Genet Date: 1980-05 Impact factor: 5.699

8. Hybrid genes in the analysis of transformation conditions : I. Setting up a simple method for direct gene transfer in plant protoplasts.

Authors: I Negrutiu; R Shillito; I Potrykus; G Biasini; F Sala
Journal: Plant Mol Biol Date: 1987-09 Impact factor: 4.076

9. Transformation of sugarcane protoplasts by direct uptake of a selectable chimaeric gene.

Authors: W H Chen; K M Gartland; M R Davey; R Sotak; J S Gartland; B J Mulligan; J B Power; E C Cocking
Journal: Plant Cell Rep Date: 1987-07 Impact factor: 4.570

10. Transient expression of electroporated DNA in monocotyledonous and dicotyledonous species.

Authors: R M Hauptmann; P Ozias-Akins; V Vasil; Z Tabaeizadeh; S G Rogers; R B Horsch; I K Vasil; R T Fraley
Journal: Plant Cell Rep Date: 1987-07 Impact factor: 4.570

6 in total

Review 1. Direct DNA transfer to plant cells.

Authors: M R Davey; E L Rech; B J Mulligan
Journal: Plant Mol Biol Date: 1989-09 Impact factor: 4.076

Review 2. Molecular improvement of cereals.

Authors: I K Vasil
Journal: Plant Mol Biol Date: 1994-09 Impact factor: 4.076

6. Direct gene transfer into Actinidia deliciosa protoplasts: analysis of transient expression of the CAT gene using TLC autoradiography and a GC-MS-based method.

Authors: M M Oliveira; J Barroso; M S Pais
Journal: Plant Mol Biol Date: 1991-08 Impact factor: 4.076