Literature DB >> 24186765

Production of transgenic sugarcane (Saccharum officinarum L.) plants by intact cell electroporation.

A Arencibia1, P R Molina, G de la Riva, G Selman-Housein.   

Abstract

We describe an efficient procedure for genetic transformation of commercial sugarcane varieties POJ 2878 and Ja 60-5. The transformation protocol is based on electroporation of a plasmid conferring GUS activity into cell clusters isolated from embryogenic calli. Six to eight weeks after electroporation, Ja 60-5 plants regenerated from electroporated tissues were tested and confirmed to be transgenic using histochemical glucuronidase and Southern hybridization analysis. Electroporation of intact cells is an efficient and reproducible method for sugarcane transformation and may also be useful for transformation of other plants.

Entities:  

Year:  1995        PMID: 24186765     DOI: 10.1007/BF00232033

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN: 0721-7714            Impact factor:   4.570


  11 in total

1.  Transgenic maize plants by tissue electroporation.

Authors:  K D'Halluin; E Bonne; M Bossut; M De Beuckeleer; J Leemans
Journal:  Plant Cell       Date:  1992-12       Impact factor: 11.277

2.  Transient gene expression in electroporated protoplasts and intact cells of sugar beet.

Authors:  K Lindsey; M G Jones
Journal:  Plant Mol Biol       Date:  1987-01       Impact factor: 4.076

3.  Sugarcane protoplasts: factors affecting division and plant regeneration.

Authors:  W H Chen; M R Davey; J B Power; E C Cocking
Journal:  Plant Cell Rep       Date:  1988-08       Impact factor: 4.570

4.  Stably transformed herbicide resistant callus of sugarcane via microprojectile bombardment of cell suspension cultures and electroporation of protoplasts.

Authors:  M K Chowdhury; I K Vasil
Journal:  Plant Cell Rep       Date:  1992-09       Impact factor: 4.570

5.  Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

Authors:  R K Saiki; D H Gelfand; S Stoffel; S J Scharf; R Higuchi; G T Horn; K B Mullis; H A Erlich
Journal:  Science       Date:  1988-01-29       Impact factor: 47.728

6.  Genetically transformed maize plants from protoplasts.

Authors:  C A Rhodes; D A Pierce; I J Mettler; D Mascarenhas; J J Detmer
Journal:  Science       Date:  1988-04-08       Impact factor: 47.728

7.  A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

Authors:  A P Feinberg; B Vogelstein
Journal:  Anal Biochem       Date:  1983-07-01       Impact factor: 3.365

8.  Transformation of Maize Cells and Regeneration of Fertile Transgenic Plants.

Authors:  W. J. Gordon-Kamm; T. M. Spencer; M. L. Mangano; T. R. Adams; R. J. Daines; W. G. Start; J. V. O'Brien; S. A. Chambers; W. R. Adams; N. G. Willetts; T. B. Rice; C. J. Mackey; R. W. Krueger; A. P. Kausch; P. G. Lemaux
Journal:  Plant Cell       Date:  1990-07       Impact factor: 11.277

9.  Silicon carbide fiber-mediated DNA delivery into plant cells.

Authors:  H F Kaeppler; W Gu; D A Somers; H W Rines; A F Cockburn
Journal:  Plant Cell Rep       Date:  1990-12       Impact factor: 4.570

10.  Transformation of sugarcane protoplasts by direct uptake of a selectable chimaeric gene.

Authors:  W H Chen; K M Gartland; M R Davey; R Sotak; J S Gartland; B J Mulligan; J B Power; E C Cocking
Journal:  Plant Cell Rep       Date:  1987-07       Impact factor: 4.570

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  11 in total

1.  Posttranscriptional gene silencing in transgenic sugarcane. Dissection Of homology-dependent virus resistance in a monocot that has a complex polyploid genome

Authors: 
Journal:  Plant Physiol       Date:  1999-04       Impact factor: 8.340

2.  A rapid and highly efficient method for transformation of sugarcane callus.

Authors:  Dwi Andreas Santosa; Roy Hendroko; Abdelazim Farouk; Ralf Greiner
Journal:  Mol Biotechnol       Date:  2004-10       Impact factor: 2.695

3.  Transgenic sugarcane plants expressing high levels of modified cry1Ac provide effective control against stem borers in field trials.

Authors:  Li-Xing Weng; Hai-Hua Deng; Jin-Ling Xu; Qi Li; Yu-Qian Zhang; Zi-De Jiang; Qi-Wei Li; Jian-Wen Chen; Lian-Hui Zhang
Journal:  Transgenic Res       Date:  2010-11-03       Impact factor: 2.788

4.  Unprecedented enhancement of transient gene expression from minimal cassettes using a double terminator.

Authors:  Getu Beyene; Marco T Buenrostro-Nava; Mona B Damaj; San-Ji Gao; Joe Molina; T Erik Mirkov
Journal:  Plant Cell Rep       Date:  2010-10-22       Impact factor: 4.570

5.  Transient and stable electrotransformations of intact black Mexican sweet maize cells are obtained after preplasmolysis.

Authors:  N Sabri; B Pelissier; J Teissie
Journal:  Plant Cell Rep       Date:  1996-09       Impact factor: 4.570

6.  Transgenic grain legumes obtained by in planta electroporation-mediated gene transfer.

Authors:  G M Chowrira; V Akella; P E Fuerst; P F Lurquin
Journal:  Mol Biotechnol       Date:  1996-04       Impact factor: 2.695

Review 7.  Gene transfer by electroporation.

Authors:  P F Lurquin
Journal:  Mol Biotechnol       Date:  1997-02       Impact factor: 2.695

Review 8.  Gene transfer to plants by electroporation: methods and applications.

Authors:  Ibrahim Ilker Ozyigit
Journal:  Mol Biol Rep       Date:  2020-04-02       Impact factor: 2.316

9.  Prospecting the utility of a PMI/mannose selection system for the recovery of transgenic sugarcane (Saccharum spp. hybrid) plants.

Authors:  Mukesh Jain; Kudithipudi Chengalrayan; Ahmed Abouzid; Maria Gallo
Journal:  Plant Cell Rep       Date:  2006-12-06       Impact factor: 4.964

10.  Sugarcane (Saccharum X officinarum): A Reference Study for the Regulation of Genetically Modified Cultivars in Brazil.

Authors:  Adriana Cheavegatti-Gianotto; Hellen Marília Couto de Abreu; Paulo Arruda; João Carlos Bespalhok Filho; William Lee Burnquist; Silvana Creste; Luciana di Ciero; Jesus Aparecido Ferro; Antônio Vargas de Oliveira Figueira; Tarciso de Sousa Filgueiras; Mária de Fátima Grossi-de-Sá; Elio Cesar Guzzo; Hermann Paulo Hoffmann; Marcos Guimarães de Andrade Landell; Newton Macedo; Sizuo Matsuoka; Fernando de Castro Reinach; Eduardo Romano; William José da Silva; Márcio de Castro Silva Filho; Eugenio César Ulian
Journal:  Trop Plant Biol       Date:  2011-02-22       Impact factor: 1.512

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