| Literature DB >> 23828890 |
Ryan Lister1,2, Eran A Mukamel3, Joseph R Nery1, Mark Urich1, Clare A Puddifoot3, Nicholas D Johnson3, Jacinta Lucero3, Yun Huang4, Andrew J Dwork5,6, Matthew D Schultz1,7, Miao Yu8, Julian Tonti-Filippini2, Holger Heyn9, Shijun Hu10, Joseph C Wu10, Anjana Rao4, Manel Esteller9,11, Chuan He8, Fatemeh G Haghighi5, Terrence J Sejnowski3,12,13, M Margarita Behrens3, Joseph R Ecker1,13.
Abstract
DNA methylation is implicated in mammalian brain development and plasticity underlying learning and memory. We report the genome-wide composition, patterning, cell specificity, and dynamics of DNA methylation at single-base resolution in human and mouse frontal cortex throughout their lifespan. Widespread methylome reconfiguration occurs during fetal to young adult development, coincident with synaptogenesis. During this period, highly conserved non-CG methylation (mCH) accumulates in neurons, but not glia, to become the dominant form of methylation in the human neuronal genome. Moreover, we found an mCH signature that identifies genes escaping X-chromosome inactivation. Last, whole-genome single-base resolution 5-hydroxymethylcytosine (hmC) maps revealed that hmC marks fetal brain cell genomes at putative regulatory regions that are CG-demethylated and activated in the adult brain and that CG demethylation at these hmC-poised loci depends on Tet2 activity.Entities:
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Year: 2013 PMID： 23828890 PMCID： PMC3785061 DOI： 10.1126/science.1237905
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728