Literature DB >> 4538516

The presence of 5-hydroxymethylcytosine in animal deoxyribonucleic acid.

N W Penn, R Suwalski, C O'Riley, K Bojanowski, R Yura.   

Abstract

A method is given for small-scale preparation of DNA from 1.0-1.5g of adult rat tissues. The product from brain or liver is characterized by base ratios and phosphorus content which accord with reported values for rat tissue. It is reasonably free of RNA, protein and glycogen. It contains 5-hydroxymethylcytosine at a content of about 15% of the total cytosine bases present. 5-Hydroxymethylcytosine is also demonstrable in mouse and frog brain DNA and in the crude cytidylic acid fractions obtained from RNA hydrolysates of rat brain and liver. 5-Hydroxymethylcytosine is identified by paper chromatography, u.v. spectra in acid and alkaline solutions and by its conversion into 5-hydroxymethyluracil.

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Year:  1972        PMID： 4538516      PMCID： PMC1178489          DOI： 10.1042/bj1260781

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  17 in total

1.  Spectrophotometric studies of nucleic acid derivatives and related compounds as a function of pH. I. Pyrimidines.

Authors:  D SHUGAR; J J FOX
Journal:  Biochim Biophys Acta       Date:  1952

2.  The composition of the deoxyribonucleic acid of salmon sperm.

Authors:  E CHARGAFF; R LIPSHITZ; C GREEN; M E HODES
Journal:  J Biol Chem       Date:  1951-09       Impact factor: 5.157

3.  The purine and pyrimidine composition of deoxypentose nucleic acids.

Authors:  G R WYATT
Journal:  Biochem J       Date:  1951-05       Impact factor: 3.857

4.  Detection of sugars on paper chromatograms.

Authors:  W E TREVELYAN; D P PROCTER; J S HARRISON
Journal:  Nature       Date:  1950-09-09       Impact factor: 49.962

5.  Composition of deoxyribonucleoprotein in human brain.

Authors:  N Robinson
Journal:  Clin Chim Acta       Date:  1966-10       Impact factor: 3.786

6.  Effect of divalent cations on the reduction and re-formation of the disulfide bonds of deoxyribonuclease.

Authors:  P A Price; W H Stein; S Moore
Journal:  J Biol Chem       Date:  1969-02-10       Impact factor: 5.157

7.  Quantitative determination of deoxyribonucleic acid in rat brain.

Authors:  N W Penn; R Suwalski
Journal:  Biochem J       Date:  1969-11       Impact factor: 3.857

8.  Comparison of the DNA's obtained from brain nuclei and mitochondria of mice and from the nuclei and kinetoplasts of Leishmania enriettii.

Authors:  H G Du Buy; C F Mattern; F L Riley
Journal:  Biochim Biophys Acta       Date:  1966-08-17

9.  The lipid composition of purified bovine brain myelin.

Authors:  W T Norton; L A Autilio
Journal:  J Neurochem       Date:  1966-04       Impact factor: 5.372

10.  Nucleic acid economy in bacteria infected with bacteriophage T2. I. Purine and pyrimidine composition.

Authors:  A D HERSHEY; J DIXON; M CHASE
Journal:  J Gen Physiol       Date:  1953-07       Impact factor: 4.086
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  110 in total

1.  A sensitive mass spectrometry method for simultaneous quantification of DNA methylation and hydroxymethylation levels in biological samples.

Authors:  Thuc Le; Kee-Pyo Kim; Guoping Fan; Kym F Faull
Journal:  Anal Biochem       Date:  2011-01-24       Impact factor: 3.365

2.  Genome-wide regulation of 5hmC, 5mC, and gene expression by Tet1 hydroxylase in mouse embryonic stem cells.

Authors:  Yufei Xu; Feizhen Wu; Li Tan; Lingchun Kong; Lijun Xiong; Jie Deng; Andrew J Barbera; Lijuan Zheng; Haikuo Zhang; Stephen Huang; Jinrong Min; Thomas Nicholson; Taiping Chen; Guoliang Xu; Yang Shi; Kun Zhang; Yujiang Geno Shi
Journal:  Mol Cell       Date:  2011-04-21       Impact factor: 17.970

Review 3.  CpG methylation in neurons: message, memory, or mask?

Authors:  Rajiv P Sharma; David P Gavin; Dennis R Grayson
Journal:  Neuropsychopharmacology       Date:  2010-07-14       Impact factor: 7.853

Review 4.  Dynamics of DNA methylation in aging and Alzheimer's disease.

Authors:  Hasan A Irier; Peng Jin
Journal:  DNA Cell Biol       Date:  2012-02-07       Impact factor: 3.311

Review 5.  Perspectives and future directions for epigenetics in hematology.

Authors:  Margaret A Goodell; Lucy A Godley
Journal:  Blood       Date:  2013-05-21       Impact factor: 22.113

6.  Tracking genomic hydroxymethylation by the base.

Authors:  Gilles Salbert; Michael Weber
Journal:  Nat Methods       Date:  2011-12-28       Impact factor: 28.547

Review 7.  High-throughput sequencing offers new insights into 5-hydroxymethylcytosine.

Authors:  Alina P S Pang; Christopher Sugai; Alika K Maunakea
Journal:  Biomol Concepts       Date:  2016-06-01

8.  Developmental changes and sex differences in DNA methylation and demethylation in hypothalamic regions of the mouse brain.

Authors:  Carla D Cisternas; Laura R Cortes; Emily C Bruggeman; Bing Yao; Nancy G Forger
Journal:  Epigenetics       Date:  2019-08-03       Impact factor: 4.528

Review 9.  5-Hydroxymethylcytosine: generation, fate, and genomic distribution.

Authors:  Li Shen; Yi Zhang
Journal:  Curr Opin Cell Biol       Date:  2013-03-13       Impact factor: 8.382

Review 10.  5-Hydroxymethylcytosine--the elusive epigenetic mark in mammalian DNA.

Authors:  Edita Kriukienė; Zita Liutkevičiūtė; Saulius Klimašauskas
Journal:  Chem Soc Rev       Date:  2012-07-27       Impact factor: 54.564
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