| Literature DB >> 24813617 |
Lu Wang1,2, Jun Zhang3, Jialei Duan1, Xinxing Gao3, Wei Zhu1,2, Xingyu Lu4, Lu Yang5, Jing Zhang1, Guoqiang Li1,2, Weimin Ci6, Wei Li7, Qi Zhou7, Neel Aluru8, Fuchou Tang5, Chuan He4, Xingxu Huang3, Jiang Liu1.
Abstract
The reprogramming of parental methylomes is essential for embryonic development. In mammals, paternal 5-methylcytosines (5mCs) have been proposed to be actively converted to oxidized bases. These paternal oxidized bases and maternal 5mCs are believed to be passively diluted by cell divisions. By generating single-base resolution, allele-specific DNA methylomes from mouse gametes, early embryos, and primordial germ cell (PGC), as well as single-base-resolution maps of oxidized cytosine bases for early embryos, we report the existence of 5hmC and 5fC in both maternal and paternal genomes and find that 5mC or its oxidized derivatives, at the majority of demethylated CpGs, are converted to unmodified cytosines independent of passive dilution from gametes to four-cell embryos. Therefore, we conclude that paternal methylome and at least a significant proportion of maternal methylome go through active demethylation during embryonic development. Additionally, all the known imprinting control regions (ICRs) were classified into germ-line or somatic ICRs.Entities:
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Year: 2014 PMID： 24813617 PMCID： PMC4096154 DOI： 10.1016/j.cell.2014.04.017
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582