Comparing influenza vaccine efficacy against mismatched and matched strains: a systematic review and meta-analysis.

BACKGROUND: Influenza vaccines are most effective when the antigens in the vaccine match those of circulating strains. However, antigens contained in the vaccines do not always match circulating strains. In the present work we aimed to examine the vaccine efficacy (VE) afforded by influenza vaccines when they are not well matched to circulating strains.
METHODS: We identified randomized clinical trials (RCTs) through MEDLINE, EMBASE, the Cochrane Library, and references of included RCTs. RCTs reporting laboratory-confirmed influenza among healthy participants vaccinated with antigens of matching and non-matching influenza strains were included. Two independent reviewers screened citations/full-text articles, abstracted data, and appraised risk of bias. Conflicts were resolved by discussion. A random effects meta-analysis was conducted. VE was calculated using the following formula: (1 - relative risk × 100%).
RESULTS: We included 34 RCTs, providing data on 47 influenza seasons and 94,821 participants. The live-attenuated influenza vaccine (LAIV) showed significant protection against mismatched (six RCTs, VE 54%, 95% confidence interval (CI) 28% to 71%) and matched (seven RCTs, VE 83%, 95% CI 75% to 88%) influenza strains among children aged 6 to 36 months. Differences were observed between the point estimates for mismatched influenza A (five RCTs, VE 75%, 95% CI 41% to 90%) and mismatched influenza B (five RCTs, VE 42%, 95% CI 22% to 56%) estimates among children aged 6 to 36 months. The trivalent inactivated vaccine (TIV) also afforded significant protection against mismatched (nine RCTs, VE 52%, 95% CI 37% to 63%) and matched (eight RCTs, VE 65%, 95% CI 54% to 73%) influenza strains among adults. Numerical differences were observed between the point estimates for mismatched influenza A (five RCTs, VE 64%, 95% CI 23% to 82%) and mismatched influenza B (eight RCTs, VE 52%, 95% CI 19% to 72%) estimates among adults. Statistical heterogeneity was low (I2 <50%) across all meta-analyses, except for the LAIV meta-analyses among children (I2 = 79%).
CONCLUSIONS: The TIV and LAIV vaccines can provide cross protection against non-matching circulating strains. The point estimates for VE were different for matching versus non-matching strains, with overlapping CIs.

Background

Influenza is a major public health threat. It is widely accepted that an annual influenza vaccination is the most effective way to prevent influenza [1]. Among adults, for example, the inactivated influenza vaccine was reported to have a vaccine efficacy (VE) of 59% (95% confidence interval (CI) 51 to 67) [2].

Recommendations for influenza vaccine composition currently include two type A influenza strains (A-H3N2 and A-H1N1) and one type B influenza strain, which are updated annually. Strains are selected by scientists convened by the World Health Organization (WHO) [3] in the months before the next epidemic season is expected. This procedure includes consideration of antigenic mismatch between vaccine strains and actual epidemic strains.

Although it is accepted that matched strains provide the best protection, data on the protective efficacy of unmatched strains, or cross protection, are sparse. These data are of particular importance, given that influenza B vaccine strains did not match circulating strains in six influenza seasons between 2000 and 2011 in the USA [4]. Mismatched seasons may lead to reduced uptake of influenza vaccination, reduced VE, and more severe influenza epidemics. Therefore, estimating prevention that can be achieved during mismatched influenza seasons is of prime public health importance. This information cannot be gleaned from previous reviews on the efficacy of the influenza vaccines because they did not specifically examine vaccine efficacy for mismatched seasons [5-7]. As such, in the present work we aimed to determine the cross protection against laboratory-confirmed influenza through vaccination.

Methods

Protocol

The Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) statement was used to guide the reporting and conduct of this review [8]. A systematic review protocol was compiled and circulated to experts in influenza, systematic reviews, and statistics. The protocol was registered with PROSPERO, an international registry for systematic reviews (CRD42012001926) and published in an open-access journal [9].

Eligibility criteria

Studies reporting the incidence of influenza infection after vaccination among healthy individuals were included. The primary outcome was the incidence of laboratory-confirmed influenza verified by polymerase chain reaction (PCR) or viral culture. The secondary outcome was the incidence of laboratory-confirmed influenza verified by at least a fourfold rise in hemagglutinin inhibition (HI) antibody titers at the end of the influenza season versus baseline (serologic assay) or serologic assay in combination with another detection method (for example, viral culture or PCR). Randomized clinical trials (RCTs) and quasi-RCTs (that is, use of non-random methods to allocate patients to the treatment and control groups, such as consecutive enrolment or the last digit of a health card number) comparing any influenza vaccine versus placebo and disseminated in the English language were included.

Study selection process

The eligibility criteria were pilot tested on a random sample of 50 citations and clarified, as needed. Two reviewers subsequently screened titles and abstracts (citations) from the literature search in duplicate. Conflicts were resolved through team discussion. A similar process was followed for screening potentially relevant full-text articles identified through citation screening.

Information sources and search

The full search strategy is reported elsewhere [9]. Briefly, RCTs were identified by searching three Cochrane reviews on influenza vaccines [5-7], MEDLINE, EMBASE, Cochrane Central Register of Controlled Trials, meta-Register (a clinical trials registry), and the references of included trials. The literature searches were conducted by an experienced librarian on 31 January 2012.

Data items and collection process

The data abstracted included study characteristics, participant characteristics, and number of influenza cases per treatment group, confirmed by viral culture, PCR or serologic assay. A data abstraction form was developed and pilot tested. Two reviewers subsequently abstracted all of the data in duplicate. Discrepancies were resolved by discussion. Trial authors were contacted for data clarifications and additional unpublished data was received from six included published RCTs [10-15].

Risk of bias appraisal

The Cochrane Risk of Bias seven-item tool was used to appraise the likelihood that the RCT results were affected by bias [16]. For the selective outcome reporting criterion, trial protocols were obtained and the outcomes reported in the protocol were compared to those reported in the final trial publication. For the other sources of bias criterion, industry-funded RCTs were scored as ‘unclear’, due to the potential for funding bias [17].

Methodological issues

We also assessed other methodological issues identified a priori that are related to influenza and may have influenced the RCT results. These included case definitions used by health care workers to identify influenza-like illness (ILI), use of a surveillance system to monitor influenza cases, and randomization scheme across multisite trials. Case definition of influenza-like illness was defined according to the Centers for Disease Control and Prevention (CDC) as fever >37.8°C (100°F) with cough and/or sore throat [18]. Surveillance system was defined as a system to track ILI in the population and consisted of health care workers contacting participants to monitor ILI symptoms. Randomization scheme referred to the consistent use of an allocation schedule across trial study sites for multisite trials.

Characterization of matched or mismatched vaccine strains

The process of characterizing matched versus mismatched strains was determined a priori and involved the two steps outlined below.

Step 1

Viral strains from influenza cases in the included RCTs were characterized through HI assays using vaccine strains as the reference. Influenza A strains from infected trial participants were matched with the strain in the vaccine if they belonged to the same A subtype (that is, H1N1 or H3N2) and were antigenically similar in the HI assay (that is, if they showed sufficient crossreaction in a HI chessboard table using ferret antisera; for example, with a HI typing quotient Influenza A viral strains were considered mismatched by antigenic drift if they were antigenically distinct from influenza A strains contained in the vaccine as per HI typing (for example, HI titer quotient ≥fourfold) or the characterization did not belong to a similar influenza A subtype contained in the vaccine (for example, H1N1 strains circulating but only H3N2 strains contained in the vaccine for bivalent vaccines with one H subtype).

For influenza B, the epidemiological situation is more complex. In recent years, there have been two coexisting phylogenetic influenza B lineages: B/Victoria and B/Yamagata [19,20]. Influenza B strains from infected trial participants were considered matched if the strain belonged to the same lineage and were antigenically similar to the vaccine strain as per HI typing (for example, HI typing quotient influenza B mismatches, two different forms were considered. Mismatch by antigenic drift refers to strains of the same lineage that were antigenically distinct from influenza B strains contained in the vaccine as per HI typing (for example, HI quotient ≥fourfold titer), whereas mismatch by lineage refers to influenza B strains of different lineages. Whenever the influenza B lineage was not presented in the trial report, categorization was based on the influenza phylogenetic tree and verified by influenza experts on the team.

Step 2

When antigenic characterization of viral strains of infected participants was not reported, data from surveillance systems were used to determine circulating strains that occurred during the time and location of the trial conduct. These included WHO weekly epidemiological records, Mortality and Morbidity Reports Weekly (MMRW), Chinese National Influenza Center, and CDC influenza summary reports. These data were sometimes captured through correspondence with trial authors.

Synthesis of included studies

The relative risk (RR) was calculated for the number of influenza cases per treatment group for included RCTs and 95% confidence intervals (CIs) were derived based on a normal approximation. Meta-analysis was conducted using a random effects model [21] for the live attenuated influenza vaccines (LAIV), trivalent inactivated vaccines (TIV), and other vaccines, separately. A post hoc sensitivity analysis was also conducted to examine the influence of categorizing the vaccines as being either an inactivated influenza vaccine or a live influenza vaccine. VE was derived based on pooled RRs using the following formula: (1 - RR) × 100%. Only influenza infections due to mismatched strains were included in the mismatched analysis, while only influenza infections due to matched strains were included in the matched analysis.

To assess for clinical and methodological heterogeneity, we examined the forest plots from the meta-analysis. The similarity between studies regarding participant and study characteristics was considered and subgroup analysis was conducted. Subgroup analyses determined a priori included age group (children <18 years of age, adults ≥18 years of age, older patients ≥65 years of age) and type of influenza (A versus B). Statistical heterogeneity was examined using the I2 statistic [22]. Funnel plots were performed to identify potential publication bias [23]. All analyses were conducted in SAS version 9.1 (SAS Inc., Cary, NC, USA).

Results

Literature search

The literature search resulted in a total of 1,356 citations, of which 308 were deemed potentially relevant (Figure 1). Reasons for exclusion at the full-text level of screening included not laboratory-confirmed influenza (108/273), no comparison to placebo (82/273), not a RCT (68/273), not a healthy population (11/273), not vaccinated against influenza A or B (3/273), and not disseminated in English (1/273). A total of 34 trials fulfilled the inclusion criteria [10-15,24-51] plus 1 companion report [52], which was used for supplementary data only (hence a total of 35 RCTs). In all, 32 RCTs [10-15,25-27,29-51] and 2 quasi-RCTs [24,28] provided data on 47 influenza seasons including 94,821 healthy participants.

Figure 1

Study flow. This figure displays the flow of titles/abstracts and full-text studies through the systematic review.

Trial characteristics

The trials were conducted between 1970 and 2009 (Table 1) in North America (22/34), Europe (3/34), Australia (1/34), Asia (3/34), and a mixture of multisite trials in North America, Europe, South America, Asia, and South Africa (4/34; Additional file 1). A total of 11 trials examined the TIV versus placebo [15,28,30,34,38,39,44-46,49],[50], 7 examined the LAIV versus placebo [10,35,37,40-43] and 6 examined both TIV and LAIV vaccines versus placebo [11,13,14,31,36,47]. Other vaccine types were examined in several RCTs, such as monovalent inactivated [24,25,51], bivalent attenuated [26,27], whole-virus vaccine [29], baculovirus-expressed HI influenza vaccine (rHAO) [12], and a trivalent recombinant HI protein vaccine [48]. One RCT examined both a TIV and bivalent vaccine compared to placebo [32].

Table 1

Antigenic characterization of viral strains

Author (year)	Vaccine type	Vaccine composition	Type of laboratory-confirmed influenza used in the analysis	Circulating strains	Antigenic characterization as per author	Classification of laboratory-confirmed influenza viral strains as being matched	Classification of laboratory-confirmed influenza viral strains as being mismatched
Leibovitz (1971) [24]	Other (MIV)	A/Hong Kong/Aichi/68 (H3N2)	Culture	A/Hong Kong/Aichi/68 (H3N2)	Typed by HI (cut-off: fourfold rise) to A/Hong Kong/Aichi/68, B/Massachusetts/66	A/Hong Kong/Aichi/68 (H3N2)	NA
Beutner (1979) [25]	Other (MIV)	A/Port Chalmers/1/73 (H3N2)	HI assay (secondary analysis)	A/Port Chalmers, A/Victoria strains	Typed by HI (cut-off: eightfold rise) to A/Port Chalmers and A/Victoria	A/Port Chalmers/1/73 (H3N2)	A/Victoria (H3N2, classified as antigenic drift)
Rytel (1977) [26]	Other (bivalent attenuated vaccine)	A/England/42/72 (H3N2), B/Hong Kong/5/72	Culture	A/Port Chalmers/1/73 (H3N2)	Typed by HI (cut-off: fourfold rise) to A/Hong Kong/8/66, A/England/42/72, A/Port Chalmers/1/73	NA	A/Port Chalmers/1/73 (H3N2) (reported as antigenic drift)
Monto (1982) [27]	Other (bivalent attenuated vaccine)	B/Ann Arbor/1/66, B/Hong Kong/8/73	HI assay (secondary analysis)	B/Singapore/79, B/Buenos Aires/79-like	Typed by HI (cut-off: fourfold rise) to B/Hong Kong (supplied by CDC) B/Tecumseh	NA	B/Singapore/79-like, B/Buenos Aires/79-like (reported as antigenic drift)
Tannock (1984) [28]	TIV	A/Brazil/11/78 (H1N1), A/Bangkok/I/79 (H3N2), B/Singapore/222/79	HI assay (secondary analysis)	A/H3N2 and influenza B strains were circulating (unspecified)	Typed by HI (cut-off: fourfold rise) to A/Bangkok/1/79	A/Bangkok/1/79 (H3N2)	NA
Keitel (1997) Y1a[29]; Keitel (1988) [52]	Other (WV)	A/Brazil/11/78 (H1N1), A/Philippines/2/82 (H3N2), B/Singapore/222/79	Culture	A/Victoria/7/83 (H1N1), B/USSR/100/83	Antigenic characterization not actively performed; based on predominant epidemic viruses	NA	A/Victoria/7/83 (H1N1) B/USSR/100/83 (classified as antigenic drift)
Keitel (1997) Y2a[29]; Keitel (1988) [52]	Other (WV)	A/Chile/1/83 (H1N1), A/Philippine/2/82 (H3N2), B/USSR/100/83	Culture	A/Philippines/2/82 (H3N2)	Antigenic characterization not actively performed; based on predominant epidemic viruses	A/Philippines/2/82 (H3N2)	NA
Keitel (1997) Y3a[29]; Keitel (1988) [52]	Other (WV)	A/Chile/1/83 (H1N1), A/Philippine/2/82 (H3N2), B/USSR/100/83	Culture	A/Mississippi/1/85 (H3N2), B/Ann Arbor/1/86	Antigenic characterization not actively performed; based on predominant epidemic viruses	NA	B/Ann Arbor/1/86 (classified as antigenic drift)
Keitel (1997) Y4a[29]; Keitel (1988) [52]	Other (WV)	A/Chile/1/83 (H1N1), A/Mississippi/1/85 (H3N2), B/Ann Arbor/1/86 (Victoria) plus A/Taiwan/1/86 (H1N1, monovalent supplement)	Culture	A/Taiwan/1/86 (H1N1)	Antigenic characterization not actively performed; based on predominant epidemic viruses	A/Taiwan/1/86 (H1N1)	NA
Keitel (1997) Y5a[29]; Keitel (1988) [52]	Other (WV)	A/Leningrad/360/86 (H3N2), A/Taiwan/1/86 (H1N1), B/Ann Arbor/1/86 (Victoria)	Culture	A/Sichuan/1/87 (H3N2)B/Victoria/2/87 (Victoria)	Antigenic characterization not actively performed; based on predominant epidemic viruses	NA	A/Sichuan/1/87 (H3N2), B/Victoria/2/87 (classified as antigenic drift
Gruber (1990) [30]	TIV	A/Chile/1/83 (H1N1), A/Philippines/2/82 (H3N2), B/USSR/100/83 (lineage unknown)	Culture or HI assay (secondary analysis)	B/Ann Arbor/1/86 (Victoria)	Typed by HI (cut-off: fourfold rise) to A/Chile, A/Philippines, A/Dunedin, A/Panama B/Ann Arbor, B/USSR	NA	B/Ann Arbor/1/86 (reported as antigenic drift)
Edwards (1994) Y1 [31]	LAIV, TIV	LAIV: A/Texas/1/85 (H1N1), A/Bethesda/1/85 (H3N2); TIV: A/Chile/1/83 (H1N1), A/Mississippi/1/85 (H3N2), B/Ann Arbor/1/86	Culture	A/Taiwan/1/86 (H1N1)	Typed by HI (cut-off: fourfold rise) by to the circulating strains and strains in the vaccine (supplied by CDC)	NA	A/Taiwan/1/86 (H1N1) (reported as antigenic drift)
Edwards (1994) Y2 [31]	LAIV, TIV	LAIV: A/Kawasaki/9/86 (H1N1), A/Bethesda/1/85 (H3N2); TIV: A/Taiwan/1/86 (H1N1), A/Leningrad/360/86 (H3N2), B/Ann Arbor/1/86	Culture	A/Sichuan/2/87 (H3N2), B/Victoria/2/87 (Victoria)	Typed by HI (cut-off: fourfold rise) by to the circulating strains and strains in the vaccine (supplied by CDC)	NA	A/Sichuan/2/87 (H3N2) (reported as antigenic drift)
Edwards (1994) Y3 [31]	LAIV, TIV	LAIV: A/Kawasaki/9/86 (H1N1), A/Los Angeles/2/87 (H3N2); other: A/Taiwan/1/86 (H1N1), A/Sichuan/2/87 (H3N2), B/Beijing/1/87	Culture	A/Taiwan/1/86 (H1N1), B/Yamagata/16/88 (Yamagata)	Typed by HI (cut-off: fourfold rise) by to the circulating strains and strains in the vaccine (supplied by CDC)	A/Taiwan/1/86 (H1N1)	NA
Edwards (1994) Y4 [31]	LAIV, TIV	LAIV: A/Kawasaki/9/86 (H1N1), A/Los Angeles/2/87 (H3N2); TIV: A/Taiwan/1/86 (H1N1), A/Shanghai/11/87 (H3N2), B/Yamagata/16/88	Culture	A/Shanghai/11/87 (H3N2)	Typed by HI (cut-off: fourfold rise) by to the circulating strains and strains in the vaccine (supplied by CDC)	A/Shanghai/11/87 (H3N2)	NA
Clover (1991) [32]	TIV, Other (Bivalent CR)	TIV: A/Chile/83 (H1N1), A/Mississippi/85 (H3N2), B/Ann Arbor/8 (Victoria); bivalent CR: A/Texas/1/85 (H1N1), A/Bethesda/1/85 (H3N2)	Viral culture or HI assay (secondary analysis)	A/Taiwan/1/86 (H1N1) closely related to A/Singapore/6/86 (H1N1), A/Chile/1/83 (H1N1)	Typed by HI (cut-off: eightfold rise or two ≥fourfold rises) to A/Chile and A/Taiwan	NA	A/Taiwan/1/86-like (H1N1)
Govaert (1994) [33]	Other (purified split viron vaccine)	A/Singapore/6/86 (H1N1), A/Beijing/353/89 (H3N2), B/Beijing/1/87 (Victoria), B/Panama/45/90 (Yamagata)	HI assay (secondary analysis)	A/Beijing/353/89 (H3N2), A/Singapore/6/86 (H1N1), B/Beijing/1/87 (Victoria), B/Panama/45/90 (Yamagata)	Antigenic characterization not actively performed; based on predominant epidemic viruses	A/Beijing/353/89 (H3N2)	NA
Powers (1995) [34]	TIV	A/Texas/36/91 (H1N1), A/Beijing/32/92 (H3N2), B/Panama/45/90 (Yamagata)	Culture or HI assay (secondary analysis)	A/Beijing/32/92 (H3N2)	Type by HI (cut-off: fourfold rise) to A/Beijing (supplied by CDC)	A/Beijing/32/92 (H3N2)	NA
Belshe (1998) [35]	LAIV	A/Texas/36/91-like (H1N1), A/Wuhan/359/95-like (H3N2), B/Harbin/7/94-like (Yamagata)	Culture	Influenza A (H3N2), influenza B. The isolation of influenza A or B among the study population paralleled that in the community in general.	Typed by HI (cut-off: fourfold rise) to A/Texas, A/Wuhan, B/Harbin	A/Wuhan/359/95-like (H3N2), B/Harbin/7/94-like (Yamagata)	NA
Rudenko (2001) [36]	LAIV, TIV	LAIV: A/Leningrad/134/17/57 (H3N2) or A/Texas/36/91(H1N1), A/Nanchang/933/95 (H3N2), B/Harbin/07/94 (Yamagata), B/Ann Arbor/1/86 (Victoria); TIV: A/Texas/36/91 (H1N1), A/Nanchang/933/95 (H3N2), B/Harbin/07/94 (Yamagata)	Culture or HI assay (secondary analysis)	Viral isolates similar to A/Texas/36/91 (H1N1), or B/Harbin/07/94	Typed by HI (cut-off: fourfold rise) to vaccine strains	A/Texas/36/91 (H1N1), B/Harbin/07/94 (Yamagata)	NA
Belshe (2000) [37]	LAIV	A/Shenzhen/227/95-like (H1N1), A/Wuhan/359/95 (H3N2), B/Harbin/7/94-like	Culture	A/Sydney/5/97-like; A/Wuhan/359/95-like and Influenza B	Typed by HI (cut-off: fourfold rise) to A/Nanchang (similar to A/Wuhan) and A/Sydney	A/Wuhan/359/95-like (H3N2)	A/Sydney/5/97-like (H3N2) (reported as antigenic drift)
Bridges (2000) Y1 [38]	TIV	A/Johannesburg/82/96 (H1N1), A/Nanchang/933/95 (H3N2), B/Harbin/7/94 (Yamagata)	Culture or HI assay (secondary analysis)	A/Sydney/5/97-like (H3N2), A/Michigan/8/98 (H3N2)	Typed by HI (cut-off: fourfold rise) to A/Johannesburg, A/Nanchang, B/Harbin, A/Sydney, A/Michigan (supplied by CDC)		A/Sydney/5/97-like (H3N2) (reported as antigenic drift)
Bridges (2000) Y2 [38]	TIV	A/Beijing/262/95 (H1N1), A/Sydney/5/97 (H3N2), and B/Harbin/7/94 (Yamagata)	Culture or HI assay (secondary analysis)	A/Sydney/5/97-like (H3N2), B/Beijing/184/93-like (Yamagata)	Typed by HI (cut-off: fourfold rise) to A/Johannesburg, A/Nanchang, B/Harbin, A/Sydney, A/Michigan (supplied by CDC)	A/Sydney/5/97-like (H3N2) B/Beijing/184/93-like (Yamagata)	NA
Hoberman (2003) Y1 [39]	TIV	A/Beijing/262/95 (H1N1), A/Sydney/15/97 (H3N2), and B/Yamanashi/166/98 (Yamagata)	Culture	A/Beijing/262/95 (H1N1), A/Sydney/15/97 (H3N2)	Typed by HI (cut-off: fourfold rise) to A/Beijing and A/Sydney	A/Beijing/262/95 (H1N1), A/Sydney/15/97 (H3N2)	NA
Hoberman (2003) Y2 [39]	TIV	A/New Caledonia/20/99 (H1N1), A/Panama/07/99 (H3N2), and B/Yamanashi/166/98 (Yamagata)	Culture	A/New Caledonia/20/99 (H1N1), A/Panama/07/99 (H3N2) B/Yamanashi/166/98 (Yamagata)	Typed by HI (cut-off: fourfold rise) to A/New Caledonia, B/Yamanashi, A/Panama	A/New Caledonia/20/99 (H1N1), A/Panama/07/99 (H3N2), B/Yamanashi/166/98 (Yamagata)	NA
Tam (2007) Y1 [40]	LAIV	A/New Caledonia/20/99 (H1N1), A/Sydney/05/97 (H3N2), B/Yamanashi/166/98 (Yamagata)	Culture	B/Sichuan/379/99 (Yamagata), B/Hong Kong/330/01-like (Victoria) (reported in Belshe review [59])	Typed by HI (cut-off: fourfold rise) to A/New Caledonia, A/Sydney, B/Yamanashi (supplied by the CDC)	Influenza A strains antigenically similar to vaccine (strains unspecified)	B/Sichuan-like (reported as antigenic drift), B/Hong Kong-like (classified as lineage mismatch as per Belshe review [59])
Tam (2007) Y2 [40]	LAIV	A/New Caledonia/20/99 (H1N1), A/Panama/2007/99 (H3N2), and B/Yamanashi/166/98 (Yamagata)	Culture	B/Victoria/504/00 (Victoria lineage) (B/Sichuan/379/99-like) virus (Yamagata)	Typed by HI (cut-off: fourfold rise) to A/New Caledonia/20/9 A/Sydney/5/97, B/Victoria/2/87 (supplied by CDC)	NA	B/Sichuan-like (reported as antigenic drift), B/Hong Kong-like (classified as lineage mismatch as per Belshe review [59])
Vesikari (2006) Y1 [41]	LAIV	A/New Caledonia/20/99 (H1N1), A/Sydney/05/97 (H3N2), B/Yamanashi/166/98 (Yamagata)	Culture	A/New Caledonia/20/99-like (H1N1) A/Panama/07/99-like (H3N2) B/Sichuan/379/99-like (Yamagata)	Typed by HI (cut-off NR) to vaccine strains and community acquired strains	A/New Caledonia/20/99-like (H1N1), A/Panama/07/99-like (H3N2)	Influenza A strains (unspecified; reported as antigenic drift), B/Sichuan-like (classified as antigenic drift as per Belshe review [59]), influenza B strains (unspecified; reported as lineage mismatch)
Vesikari (2006) Y2 [41]	LAIV	A/New Caledonia/20/99 (H1N1), A/Panama/07/99 (H3N2), B/Victoria/504/00 (Yamagata)	Culture	A/New Caledonia/20/99-like (H1N1), A/Panama/07/99-like (H3N2), B/Hong Kong/330/01-like (Victoria)	Typed by HI (cut-off NR) to vaccine strains and community acquired strains	A/New Caledonia/20/99-like (H1N1), A/Panama/2007/99-like (H3N2), B/Victoria/504/00-like (Yamagata)	B/Hong Kong-like (reported as lineage mismatch)
Forrest (2008) [42]	LAIV	A/New Caledonia/20/99-like (H1N1), A/Panama strain (H3N2), B/Victoria/504/00 (Yamagata)	Culture	A/New Caledonia/20/99 (H1N1), A/Fujian/411-02-like, B/Hong Kong/330/01-like (Victoria)	Typed by HI (cut-off: fourfold rise) to vaccine strains and community acquired strains	Antigenically similar strains to H3N2 (unspecified)	Influenza B strains (unspecified; reported as lineage mismatch)
Bracco Neto (2009) Y1b[10]	LAIV	A/New Caledonia/20/99 -like (H1N1), A/Moscow/10/99-like (H3N2), B/Sichuan/379/99-like (Yamagata)	Culture	A/New Caledonia/20/99 (H1N1), A/Panama/07/99-like (H3N2), B/Sichuan/379/99-like (Yamagata)	Typed by HI (cut-off: fourfold rise) to vaccine strains and community acquired strains (unspecified)	A/New Caledonia/20/99-like (H1N1), A/Panama/2007/99-like (H3N2), B/Yamanashi/166/98-like (Yamagata), B/Victoria/504/00-like (Yamagata)	Influenza A strains (unspecified; reported as antigenic drift), influenza B strains (unspecified; reported as lineage mismatch)
Bracco Neto (2009) Y2b[10]	LAIV	A/New Caledonia/20/99 (H1N1) A/Moscow/10/99 (H3N2), B/Hong Kong/330/01-like (Victoria)	Culture	A/New Caledonia/20/99 (H1N1), A/Panama/07/99-like (H3N2) B/Hong Kong/330/01-like (Victoria)	Typed by HI (cut-off: fourfold rise) to vaccine strains and community acquired strains (unspecified)	A/New Caledonia/20/99-like, A/Panama/07/99-like, B/Victoria-like (Victoria)	B/Hong Kong (reported as lineage mismatch)
Lum (2010) [43]	LAIV	A/New Caledonia/20/99 (H1N1), A/Panama/07/99 (H3N2), B/Hong Kong/330/2001 (Victoria)	Culture	A/New Caledonia/20/99-like (H1N1), A/Panama/07/99-like (H3N2), A/Fujian/411-02-like (H3N2) in Asian regions, B/Victoria-like (Victoria)	Typed by HI (cut-off: fourfold rise) and PCR sequencing to A/Fujian/411/02-like, B/Victoria/504/00-like, A/New Caledonia/20/99-like, A/Panama/2007/99-like, B/Hong Kong/1351/02-like (supplied by CDC and Wyeth)	A/New Caledonia-like (H1N1), A/Panama-like (H3N2), B/Hong Kong-like (Victoria)	A/Fujian-like and B/Victoria-like (Victoria) (report as antigenic drift)
Langley (2011) [44]	TIV	A/New/Caledonia/20/99 (H1N1), A/Panama/07/99 (H3N2) B/Shangdong/7/97 (Victoria)	Culture	A/New Caledonia/20/99 (H1N1)-like. A/Fujian (H3N2), B/Hong Kong/330/01-like (Victoria), B/Sichuan/379/99-like (Yamagata)	Typed by HI (cut-off: fourfold rise) (not specified)		A/Fujian (reported as antigenic drift accounting for 96.8% of H3N2 isolates)
Ohmit (2006)b[11]	LAIV, TIV	LAIV: A/New Caledonia/20/99 (H1N1), A/Wyoming/03/03-like (H3N2), B/Jilian/20/03-like (Yamagata lineage); TIV: A/New Caledonia/20/99 (H1N1), A/Wyoming/3/03 (H3N2), B/Jiangsu/10/03-like (Yamagata lineage)	Culture	A/California/07/04-like, A/Wisconsin-like (H3N2), B/Hawaii/33/04-like (Victoria lineage)	Typed by HI (cut-off: fourfold rise) to vaccine strains and A/California/07/04, B/Hawaii/33/04-like (supplied by the CDC)	B/Shanghai/361/02-like (Yamagata)	A/California/07/04-like (H3N2) (report as antigenic drift), B/Hawaii/33/04-like (report as lineage mismatch)
Treanor (2007)b[12]	Other (purified rHAO vaccine)	A/New Caledonia/20/99 (H1N1), A/Wyoming/3/03 (H3N2), B/Jiangsu/10/03 (Yamagata lineage)	Culture	A/California/7/04 -like (H3N2), A/Wisconsin-like (H3N2), B/Yamagata/16/88-like (Yamagata)	Typed by HI (cut-off: fourfold rise) to vaccine and circulating strains (unspecified) (supplied by CDC)	NA	A/California-like (H3N2, report as antigenic drift), B/Yamagata-like (report as lineage mismatch)
Beran (2009) [45]	TIV	A/New Caledonia/20/99 IVR-116 virus (H1N1), A/New York/55/04 X-157 (H3N2) B/Jiangsu/10/03 (Yamagata lineage)	Culture	A/New Caledonia/20/99 (H1N1), A/California,//07/04-like, B/Jiangsu/10/2003-like viruses (Yamagata lineage)	Typed by HI (cut-off: fourfold rise) to unspecified strains	A/California-like (H3N2), A/New Caledonia-like (H1N1), A/Czech Republic-like (H1N1)	B/Hong Kong-like, (reported as lineage mismatch)
Jackson (2010) Y1 [15]	TIV	A/New Caledonia/20/99 (H1N1), A/New York/55/04 (H3N2), B/Jiangsu/10/03 (Yamagata lineage)	Culture	A/New Caledonia/20/99 A (H1N1), A/California/07/04-like (H3N2), A/Wisconsin-like (H3N2), B/Shanghai/361/02 (Yamagata), B/Florida/07/04-like (lineage unknown) B/Ohio/1/05 (Victoria)	Typed by HI (cut-off: fourfold rise) to vaccine and circulating strains (unspecified) (supplied by CDC and WHO)	Report results for influenza A and B strains (unspecified) with ≤fourfold difference in HI titer compared to vaccine strains	Report results for influenza A and B strains (unspecified) with >fourfold difference in HI titer compared to vaccine strains
Jackson (2010) Y2 [15]	TIV	A/New Caledonia/20/99 (H1N1), A/Wisconsin/67/05 (H3N2), B/Malaysia/2506/04 (Victoria lineage)	Culture	A/New Caledonia/20/99, A (H1N1), A/Solomon Islands/3/06 A (H1N1), A/Wisconsin/67/05 A (H3N2), B/Yamagata/16/88 (Yamagata) B/Ohio/01/05 (Victoria)	Typed by HI (cut-off: fourfold rise) to vaccine and circulating strains (unspecified) (supplied by CDC and WHO)	Report results for influenza A and B strains (unspecified) with ≤fourfold difference in HI titer compared to vaccine strains	Report results for influenza A and B strains (unspecified) with >fourfold difference in HI titer compared to vaccine strains
Ohmit (2008)b[13]	LAIV, TIV	LAIV/TIV: A/New Caledonia/20/99 (H1N1), A/New York/55/04 (H3N2), B/Jiangsu/10/03 (Yamagata)	Culture or PCR	A/California/07/04-like (H3N2), A/Wisconsin/67/05 (H3N2), B/Shanghai/361/02 (Yamagata), B/Florida/07/04-like (Yamagata), B/Ohio/01/05 (Victoria)	Typed by HI (cut-off: fourfold rise) to A/Wisconsin/67/05, B/Ohio/01/05, and vaccine strains	A/California/07/04 (H3N2)	B/Ohio/01/05-like (reported as lineage mismatch)
Beran (2009) [46]	TIV	A/New Caledonia/20/99 IVR-116 (H1N1), A/Wisconsin/67/05 (H3N2), B/Malaysia/2506/04 (Victoria)	Culture	A/New Caledonia/20/99, A/Wisconsin/67/05, B/Yamagata-like (Yamagata)	Typed by HI (cut-off: fourfold rise) to unspecified strains (supplied by CDC and WHO)	A/Wisconsin (H3N2)	A/H1N1 strains (unspecified; reported as antigenic drift), B/Yamagata-like (reported as lineage mismatch)
Monto (2009)b[14]	LAIV, TIV	LAIV/TIV: A/Solomon Islands/3/06 (H1N1), A/Wisconsin/67/05 (H3N2), B/Malaysia/2506/04 (Victoria)	PCR	A/Brisbane/59/07-like (H1N1), A/Wisconsin/67/05 (H3N2)	Typed by HI (cut-off: eightfold rise) to vaccine strains and circulating strains (supplied by CDC)	A/Wisconsin-like (H3N2)	A/Brisbane-like (report as antigenic drift), B/Yamagata-like (reported as lineage mismatch)
Frey (2010) [47]	LAIV, TIV	LAIV/TIV: A/Solomon Islands/3/06 (H1N1)-like, A/Wisconsin/67/05 (H3N2)-like, B/Malaysia/2506/04-like (Victoria)	Culture	A/Solomon Islands/3/06 (H1N1) A/Brisbane/59/07-like (H3N2) A/Wisconsin/67/05-like (H3N2), B/Florida/4/06 (Yamagata), B/Ohio/01/05 (Victoria), B/Malaysia/2506/04 (Victoria)	Typed by HI (cut-off: fourfold rise) to unspecified strains (supplied by CDC)	Report results for influenza A (H1N1, H3N2) and B strains (unspecified) with ≤fourfold difference in HI titer compared to vaccine strain	Report results for influenza A (H1N1, H3N2) and B strains (unspecified) with >fourfold difference in HI titer compared to vaccine strain
Treanor (2011) [48]	Other (FluBlok®)	A/Solomon Island/3/06 (H1N1), A/Wisconsin/67/05 (H3N2), B/Malaysia/2506/04 (Victoria)	Culture	A/Brisbane/59/07 (H1N1), A/Brisbane/10/07 (H3N2), B/Florida/04/06 (Yamagata)	Typed by HI (cut-off: fourfold rise) to strains (unspecified)	A/Wisconsin/67/05-like (H3N2)	A/Brisbane/59/07-like (H1N1) (reported as antigenic drift), B/Florida/04/06 (reported as lineage mismatch) (data NR by treatment group)
Barrett (2011) [49]	TIV	A/Brisbane/59/07 (H1N1), A/Uruguay/716/07, B/Florida/4/06 (Yamagata)	Culture or RT-PCR	A/Brisbane/59/07 B/Florida/04/06 (Yamagata) B/Victoria/02/87 (Victoria)	Typed by HI (lowest titer at which the sum of the specificity and sensitivity was maximum) to strains (unspecified) (supplied by CDC)	A/Brisbane/59/07 (H1N1), A/Uruguay/716/07 (H3N2), B/Florida/4/06	Influenza A strains (unspecified), B/Victoria-like (reported as lineage mismatch)
Cowling (2010) [50]	TIV	A/Brisbane/59/07 (H1N1)-like, A/Brisbane/10/07 (H3N2)-like, and B/Florida/4/06 (Yamagata)	RT-PCR	A/Perth/16/09-like (H3N2)	Typed by HI (cut-off: fourfold rise) to A/Brisbane (H1N1), A/Brisbane (H3N2), A/California, A/Perth, H1N1 and B/Florida	A/Brisbane (H1N1), A/Brisbane (H3N2), A/California, and B/Florida	A/Perth/16/09-like (H3N2) (report as antigenic drift) and pandemic A/H1N1
Talaat (2010) [51]	Other (MIV)	A/H1N1/2009	RT-PCR	H1N1	Typed by HI (cut-off: fourfold rise) to A/H1N1/09	NA	A/H1N1 (HIN1)

Circulating strains refers to the influenza viral strains circulating in the specified time and region as reported in the publication. Circulating viral strains were crossreferenced with WHO weekly epidemiological records, Chinese National Influenza Centre CDC influenza summary reports, Mortality and Morbidity Reports Weekly (MMRW, published by CDC), where applicable. In all cases circulating strains matched influenza documentation as noted above. Antigenic characterization as per author refers to how the author determined and classified influenza strains in participants with laboratory-confirmed influenza. Immunogenicity to vaccine and non-vaccine strains conducted using cut-off values to determine match or mismatch between strains contained in the vaccine and those that were circulating. Classification of laboratory-confirmed influenza viral strains as being matched refers to the categorization of influenza strains that are antigenically similar or well matched to vaccine strains. Strains listed in the column represent break through strains that despite being well matched to vaccine caused influenza infections in the study population. In some cases, antigenically similar or well matched strains were presented in the study and are listed in the table as ‘reported’. In other cases, antigenically similar or well matched strains was determined by surveillance data as documented above, in these cases the strains are listed as ‘classified as’. Classification of laboratory-confirmed influenza viral strains as being mismatched refers to the categorization of influenza strains that are antigenically distinct or mismatched to vaccine strains. Strains listed in the column represent break through strains that were not protected through vaccination. In some cases, antigenically distinct or mismatched strains were presented in the study by authors and are listed in the table as ‘reported’. In other cases, antigenically distinct or mismatched strains was determined by subtracting well matched strains from any strains reported in the publications, these strains are listed as ‘classified as’ by authors of this review. Influenza B mismatched strains are further classified as antigenic drifts or lineage drifts. Antigenic drift represents viral strains that have mutated but are still classified within the same lineage. Lineage drifts refer to the distinction between Yamagata and Victoria lineages of influenza B. Classification of lineage was either reported by author (denoted in table as ‘reported’) or categorized based on phylogenic tree of lineages (denoted as ‘classified’).

aMain publication.

bUnpublished data was obtained from the author.

CDC Center for Disease Control and Prevention, HI haemagglutinin inhibition, LAIV live attenuated influenza vaccine, MIV monovalent inactivated vaccine, NA not applicable, NR not reported, RT-PCR reverse transcriptase polymerase chain reaction, TIV trivalent influenza vaccine, WHO World Health Organization, WV whole viral vaccine.

In all, 22 RCTs (including information on 34 influenza seasons) provided data on matched influenza strains [10,11,13-15,24,29,31,35,37],[39-43,45-51], while 20 RCTs (including data from 33 influenza seasons) provided data on mismatched influenza strains [10-15,26,29,31,37,40-47,49,50] (Table 1). Influenza infection was confirmed by culture in 20 RCTs [10-12,15,24,26,29,31,35,37],[39-48], PCR in 3 RCTs [14,50,51], and culture or PCR in 2 RCTs [13,49]. Nine RCTs included serologic assays to determine influenza infection; these were included in the secondary analysis only [25,27,28,30,32-34,36,38].

Participant characteristics

The RCTs included data on a total of 94,821 participants (Table 2). A total of 13 of the RCTs were conducted among children [10,25,30,32,35,37,39-43,49],[50], and 2 were conducted among seniors [33,36]. One RCT included children (15%) and adults (85%) [31], and another included adults and seniors [51]; the remaining RCTs were conducted among adults only. The percentage of female participants ranged from 22% [38] to 100% [26].

Table 2

Patient characteristics

Lead author (year)	Country of conduct and year	Age category	Mean age (SD) in years	M/F, %	Sample size
					Vaccine	Placebo
Leibovitz (1971) [24]	USA, 1970	Adults	NR	NR	1,682	7,934
Beutner (1979) [25]	USA, 1974	Children	Range: 7 to 14	50/50	520	460
Rytel (1977) [26]	USA, 1974	Adults	NR	0/100	95	48
Monto (1982) [27]	USA, 1979	Adults	NR	NR	144	140
Tannock (1984) [28]	Australia, 1981	Adults	34.8 (13.9)	69/31	19	20
Keitel (1997) [29]	USA, 1983 to 1988	Adults	Range: 30 to 60	NR	Y1: 161	Y1: 298
					Y2: 172	Y2: 241
					Y3: 153	Y3: 253
					Y4: 203	Y4: 217
					Y5: 121	Y5: 145
Gruber (1990) [30]	USA, 1985	Children	7.9 (3.3)	NR	54	77
Edwards (1994) [31]	USA, 1986 to 1990	Adults/Children	Range: 1 to 65	NR	LAIV/TIV
					Y1: 872/878	Y1: 878
					Y2: 1,029/1,060	Y2: 1,064
					Y3: 1,114/1,126	Y3: 1,125
					Y4: 999/1,016	Y4: 1,016
Clover (1991) [32]	USA, 1989	Children	8.8 (3.6)	NR	TIV/BIV 54/56	82
Govaert (1994) [33]	The Netherlands, 1991	Older patients	Range: 60 to 91	47/53	927	911
Powers (1995) [34]	USA, 1993	Adults	Range: 18 to 45	NR	TIV/other 26/26	24
Belshe (1998) [35]	USA, 1996	Children	3.5 (1.4)	47/53	1,070	532
Rudenko (2001) [36]	Russia, 1996	Older patients	Median: 73, Range: 41 to 95	30/70	LAIV/TIV 111/93	109
Belshe (2000) [37]	USA, 1997	Children	4.5 (1.4)	52/48	917	441
Bridges (2000) [38]	USA, 1997 to 1999	Adults	Median: 43.5	78/22	Y1: 138	Y1: 137
					Y2: 141	Y2: 137
Hoberman (2003) [39]	USA, 1999 to 2001	Children	Range: 0.5 to 2	56/44	Y1: 273	Y1: 138
					Y2: 252	Y2: 123
Tam (2007) [40]	Multisite trial in Asia, 2000 to 2002	Children	1.9 (0.6)	53/47	Y1: 1,653	Y1: 1,111
					Y2: 503	Y2: 494
Vesikari (2006) [41]	Multisite trial in Europe and Israel, 2000 to 2001	Children	2.0 (0.7)	51/49	Y1: 951	Y1: 665
					Y2: 640	Y2: 450
Bracco Neto (2009)a[10]	Multisite trial in South Africa and South America, 2001 to 2002	Children	Range: 0.5 to 3	49/51	Y1: 944	Y1: 942
					Y2: 338	Y2: 342
Forrest (2008) [42]	Multisite in Asia, 2002	Children	1.8 Range: 0.5 to 3	NR	525	516
Lum (2010) [43]	Multisite trial in Asia, Europe and South America, 2002	Children	1.2 (0.3)	50/50	765	385
Langley (2011) [44]	Canada, 2003	Adults	37.1 (12.2)	46/54	455	443
Ohmit (2006)a[11]	USA, 2004	Adult	26.9 (9.3)	38/62	LAIV/TIV 519/522	206
Treanor (2007)a[12]	USA, 2004	Adults	Median: 31, Range: 18 to 49	37/63	151	153
Beran (2009) [45]	Czech Republic, 2005	Adults	35 (13)	45/55	4,137	2,066
Jackson (2010) [15]	USA, 2005	Adults	32.7 (9.1)	40/60	Y1: 1,706	Y1: 1,725
					Y2: 2,011	Y2: 2,043
Ohmit (2008)a[13]	USA, 2005	Adults	24.9 (NR)	40/60	LAIV/TIV 853/867	338
Beran (2009) [46]	Multisite trial Europe, 2006	Adults	40.0 (13.3)	40/60	5,103	2,549
Monto (2009)a[14]	USA, 2007	Adults	23.3 (7.4)	38/62	LAIV/TIV 813/814	325
Frey (2010) [47]	Multisite trial North America and Europe, 2007	Adults	32.5 (NR)	44/45	LAIV/TIV 3,776/3,638	3,843
			Range: 18 to 48
Treanor (2011) [48]	USA, 2007	Adults	32.5(NR)	41/59	2,344	2,304
			Range: 18 to 55
Barrett (2011) [49]	Multisite trial in USA, 2008	Children	Range: 18 to 49	NR	3,619	3,617
Cowling (2010) [50]	Hong Kong, 2008	Children	Range: 6 to 15	53/47	71	48
Talaat (2010) [51]	USA, 2009	Adults and older patients	56.5 (18.0)	43/57	389	97

aUnpublished data was obtained from the author(s).

LAIV live attenuated influenza vaccine, NR not reported, TIV trivalent inactivated vaccine.

Risk of bias

Of the included trials, only 26% adequately reported sequence generation and 29% adequately reported allocation concealment (Figure 2). The majority of the RCTs blinded participants and physicians (65%) and addressed incomplete data (62%). Only half of the RCTs were deemed to be free from other sources of bias (44%; 56% scored unclear because they were industry funded) and 23% were free from selective outcome reporting (Table 3).

Figure 2

Risk of bias across all studies. This figure represents the risk of bias presented in the included studies. Green refers to a low risk of bias, yellow refers to an unclear risk of bias, and red refers to a high risk of bias.

Table 3

Risk of bias

Lead author (year)	Country of conduct and year	Adequate sequence generation	Allocation concealment	Blinding outcome assessors	Blinding patients and providers	Incomplete outcome data addressed	Free of selective reporting	Funding source
Leibovitz (1971) [24]	USA, 1970	High	Unclear	Unclear	Unclear	Low	Unclear	Low
Beutner (1979) [25]	USA, 1974	Unclear	Unclear	Unclear	Unclear	Unclear	Unclear	Low
Rytel (1977) [26]	USA, 1974	Low	Low	Unclear	Unclear	Low	Unclear	Unclear
Monto (1982) [27]	USA, 1979	Unclear	Unclear	Unclear	Unclear	Low	Unclear	Low
Tannock (1984) [28]	Australia, 1981	High	Unclear	Unclear	Low	Low	Unclear	Unclear
Keitel (1997) [29]	USA, 1983 to 1988	Unclear	Unclear	Unclear	Unclear	Unclear	Unclear	Low
Gruber (1990) [30]	USA, 1985	Unclear	Unclear	Unclear	Low	Unclear	Unclear	Low
Edwards (1994) [31]	USA, 1986 to 1990	Low	Low	Unclear	Low	Unclear	Unclear	Low
Clover (1991) [32]	USA, 1989	Unclear	Unclear	Unclear	Unclear	Unclear	Unclear	Low
Govaert (1994) [33]	The Netherlands, 1991	Unclear	Low	Unclear	Low	Low	Unclear	Low
Powers (1995) [34]	USA, 1993	Unclear	Unclear	Unclear	Low	Unclear	Unclear	Low
Belshe (1998) [35]	USA, 1996	Unclear	Unclear	Unclear	Low	Unclear	Unclear	Low
Rudenko (2001) [36]	Russia, 1996	Unclear	Unclear	Unclear	Unclear	Unclear	Unclear	Unclear
Belshe (2000) [37]	USA, 1997	Unclear	Unclear	Unclear	Low	Unclear	Unclear	Low
Bridges (2000) [38]	USA, 1997 to 1998	Unclear	Unclear	Unclear	Low	Low	Unclear	Low
Hoberman (2003) [39]	USA, 1999 to 2000	Low	Unclear	Low	High	Low	Unclear	Unclear
Tam (2007) [40]	Multisite trial in Asia, 2000 to 2001	Low	Low	Unclear	Low	Unclear	Unclear	Unclear
Vesikari (2006) [41]	Multisite trial in Europe and Israel, 2000 to 2001	Unclear	Unclear	Unclear	Low	Low	Unclear	Unclear
Bracco Neto (2009)a[10]	Multisite trial in South Africa and South America, 2001 to 2002	Low	Low	Low	Unclear	Unclear	Unclear	Unclear
Lum (2010) [43]	Multisite trial in Asia, Europe and South America, 2002	Unclear	Low	Low	Low	Low	Unclear	Unclear
Forrest (2008) [42]	Multisite in Asia, 2002	Unclear	Unclear	Unclear	Low	Unclear	Unclear	Unclear
Langley (2011) [44]	Canada, 2003	Unclear	Unclear	Low	Low	Low	Unclear	Unclear
Ohmit (2006)a[11]	USA, 2004	Unclear	Unclear	Unclear	Low	Low	Low	Low
Treanor (2007)a[12]	USA, 2004	Unclear	Unclear	Unclear	Low	Low	Low	Unclear
Beran (2009) [45]	Czech Republic, 2005	Low	Low	Unclear	Low	Low	High	Unclear
Jackson (2010) [15]	USA, 2005	Low	Low	Unclear	Unclear	Low	Low	Unclear
Ohmit (2008)a[13]	USA, 2005	Unclear	Unclear	Unclear	Low	Low	Low	Low
Beran (2009) [46]	Multisite trial Europe, 2006	Unclear	Unclear	Unclear	Low	Low	High	Unclear
Monto (2009)a[14]	USA, 2007	Unclear	Unclear	Unclear	Low	Low	Low	Unclear
Frey (2010) [47]	Multisite trial North America and Europe, 2007	Unclear	Unclear	Unclear	Unclear	Low	Low	Unclear
Treanor (2011) [48]	USA, 2007	Unclear	Unclear	Unclear	Unclear	Low	Low	Unclear
Barrett (2011) [49]	Multisite trial in USA, 2008	Low	Low	Unclear	Low	Unclear	Unclear	Unclear
Cowling (2010) [50]	Hong Kong, 2008	Low	Low	Unclear	Low	Low	Low	Low
Talaat (2010) [51]	USA, 2009	Unclear	Unclear	Unclear	Low	Low	Unclear	Unclear

aUnpublished data was obtained from the author(s).

The trials were conducted over 40 years and the risk of bias for older studies was compared to newer studies, using 1991 as the midpoint. No trends were observed for sequence generation or allocation sequence and differences between groups were minimal. Blinding of outcome assessors and the reporting of incomplete data improved in recent studies by 17%. Furthermore, selective outcome reporting in recent studies showed an improvement of 42% in the risk of bias. There was also an increase in the proportion of RCTs scoring ‘unclear’ on other risk of bias criterion, as more trials were funded by private industry in recent years.

Only 15% of trials were free of other methodological issues, which included adequate information on case definition of ILI and surveillance of influenza, as well as details regarding randomization across multiple sites [12,38,44,48,50] (Additional file 2). Almost half of trials (16 of 34) did not adequately report a case definition of ILI and the remaining studies were unclear in at least 1 of the domains for other methodological concerns.

LAIV versus placebo

The results from all LAIV versus placebo meta-analyses are presented in Additional file 3. For simplicity, we have focused on the results specific to children aged <18 years for the primary outcome.

The LAIV was protective against mismatched strains overall (11 RCTs, VE 60%, 95% CI 44% to 71%; Figure 3; Additional file 3) [10,11,13,14,31,37,40-43,47], as well as among children aged 6 to 36 months (6 RCTs, VE 54%, 95% CI 28% to 71%) [10,37,40-43] when strains were mismatched. Similarly, the LAIV was effective in protecting against infection when circulating strains were well matched overall (12 RCTs, VE 77%, 95% CI 67% to 86%, Figure 4) [10,11,13,14,31,35,37,40-43],[47]. LAIV provided significant protection among children when circulating strains matched vaccine composition (seven RCTs, VE 83%, 95% CI 75% to 88%) [10,35,37,40-43].

Figure 3

Meta-analysis of live attenuated influenza vaccine (LAIV), mismatched. This figure represents the relative risk (RR) of an influenza infection occurring when the circulating strain does not match strains contained in the LAIV.

Figure 4

Meta-analysis of live attenuated influenza vaccine (LAIV), matched. This figure represents the relative risk (RR) of an influenza infection occurring when the circulating strain matches strains contained in the LAIV.

When influenza A strains did not match LAIV composition, protection against infection was statistically significant among children (five RCTs, VE 75%, 95% CI 41% to 90%) [10,37,40,41,43]. LAIV was also effective in protecting against influenza A when strains were well matched in children (six RCTs, VE 84%, 95% CI 76% to 90%) [10,35,40-43].

When influenza B strains were mismatched, LAIV was effective among children (five RCTs, VE 42%, 95% CI 22% to 56%) [10,40-43]. Furthermore, LAIV was more protective against drifted B strains (two RCTs, VE 62%, 95% CI 21% to 81%) [40,41] than lineage mismatch for influenza B (five RCTs, VE 34%, 95% CI 4−% to 59%) [10,40-43] in children. Similarly, LAIV was effective against influenza B when circulating strains matched vaccine strains in children (four RCTs, VE 79%, 95% CI 58% to 90%) [10,35,41,43].

TIV versus placebo

The results from all TIV versus placebo meta-analyses are presented in Additional file 3. For simplicity in the text, we have focused on the results specific to adults aged ≥18 years.

The TIV showed protection against mismatched strains overall (11 RCTs, VE 56%, 95% CI 43% to 66%, Figure 5) [11,13-15,31,44-47,49,50] and among adults (9 RCTs, VE 52%, 95% CI 37% to 63%) [11,13-15,44-47,49]. Similarly, TIV vaccines showed protection against matched strains overall (11 RCTs, VE 65%, 95% CI 58% to 72%, Figure 6) [11,13-15,31,39,45-47,49,50] and among adults (8 RCTs, VE 65%, 95% CI 54% to 73%) [11,13-15,45-47,49].

Figure 5

Meta-analysis of trivalent inactivated vaccine (TIV), mismatched. This figure represents the relative risk (RR) of an influenza infection occurring when the circulating strain does not match strains contained in the TIV.

Figure 6

Meta-analysis of trivalent inactivated vaccine (TIV), matched. This figure represents the relative risk (RR) of an influenza infection occurring when the circulating strain does not match strains contained in the TIV.

In adults, TIV provided significant protection against mismatched influenza A strains (six RCTs, VE 64%, 95% CI 23% to 82%) [11,14,15,45,47,49], and matched influenza A strains (seven RCTs, VE 61%, 95% CI 46% to 73%) [13-15,45-47,49]. The TIV also afforded protection against mismatched influenza B strains (eight RCTs, VE 52%, 95% CI 19% to 72%) [11,13-15,45-47,49], as well as matched influenza B strains (four RCTs, VE 77%, 95% CI 18% to 94%) [11,15,47,49] among adults.

Other vaccines compared to placebo

In all, 3 RCTs including 5 influenza seasons and 1,578 participants provided efficacy estimates against mismatched strains for other vaccines [12,26,29,31]. Protection against mismatched strains was statistically significant (three RCTs, VE 56%, 95% CI 23% to 75%) [12,26,29].

Other vaccines contributed data on 5 influenza seasons from 4 RCTs including 15,592 participants for matched strains. Other vaccines provided a VE of 54% (four RCTs, 95% CI 5% to 78%) [24,29,48,51].

Post hoc subgroup analysis

Our results did not change after a post hoc sensitivity analysis was conducted to examine the influence of categorizing the vaccines as being either an inactivated influenza vaccine or a live influenza vaccine versus categorizing the vaccines as LAIV, TIV, and other.

Discussion

In the present work, we conducted a systematic review to estimate the protection afforded by mismatched influenza vaccines. Our results show that mismatched vaccines can reduce the risk of PCR or culture-confirmed influenza by 60% for LAIV (95% CI 44% to 71%) and by 56% for TIV (95% CI 43% to 66%). These results suggest a benefit of vaccines in preventing laboratory-confirmed influenza even when there is a mismatch between vaccine composition and circulating strains. For matched influenza, point estimates for VE were slightly higher for both the LAIV (77%, 95% CI 67% to 86%) and the TIV (65%, 95% CI 57% to 72%), versus mismatched point estimates. However, there was substantial overlap in the CIs for matched and mismatched estimates in some instances; the impact of this overlap could not be formally tested via meta-analyses techniques.

A previous systematic review on a similar topic did not report the results separately for matched or mismatched strains [2]. A Cochrane review among healthy adults found a VE for TIV non-WHO matching strains (including when the specific strains are unknown) of 44% (95% CI 23% to 59%) [6]. This result was based on six influenza seasons from four RCTs. A VE of 68% (95% CI 44% to 81%) was observed for LAIV non-WHO matching strains (including when the specific strains are unknown), based on four point estimates from three RCTs [6]. Our review is based on an additional 13 RCTs and we have included more than double the data for both LAIV and TIV compared to the Cochrane review. Furthermore, here we have followed a rigorous process of identifying matched and mismatched data, which has not been attempted in previous reviews. Our results are consistent with those found in a pooled observational study including 5 years of data [53].

We found that the LAIV was more efficacious among children versus adults, which is likely a reflection of the difference in previously acquired influenza infections between age groups and the consequently larger amount of pre-vaccination antibody, which affects the live vaccine. However, we found higher point estimates for adults versus children for mismatched LAIV estimates. This finding might suggest that there might be a possible discrepancy in the degree of matching, which may have impacted our results. Specifically, trials conducted among children may have had a greater degree of mismatch than those conducted among adults. Unfortunately, the current analysis does not allow the degree of mismatch to be examined. For the purposes of our analysis, we dichotomized cross protection but in reality, the degree of mismatch is a continuum. The cross protection inferred by mismatch strains should be analyzed as a continuum in the future.

The results from our secondary outcome analysis were often inconsistent with our primary outcome results. This inconsistency is likely due to differences in sensitivity and specificity of different laboratory tests over time. Indeed, the most reliable diagnostic test for clinical practice is PCR so the results from our primary outcome should be considered the most valid [54,55].

Our results are generalizable to seasonal influenza, as most of the included studies reported on this type of influenza. Only one of the included RCTs occurred during the influenza pandemic [51]. When this study was removed via sensitivity analysis, we did not observe any differences on our meta-analysis results.

We identified three RCTs reporting data among seniors [33,36,51], yet none reported our primary outcome of interest or provided data on mismatched influenza. This finding is consistent with previous influenza reviews, for which few RCTs were identified among this age group [2,5]. Future RCTs are needed in this area to provide patients, healthcare providers, and health policymakers with guidance related to vaccination among seniors during seasons when the vaccine composition does not match circulating strains [5].

There are a few limitations in the evidence base summarized for this review. Many of the trials had an unclear risk of bias because of poor reporting. However, we did note improvements in risk of bias over time that may be because of uptake of the Consolidated Standards of Reporting Trials (CONSORT Statement), providing guidance on what should be reported in RCTs [56]. Enhanced reporting of outcome definitions might be due to clinical trial registry requirements for trials. We also noted that more trials in this area were funded by industry over time.

Another potential limitation is that although unpublished data was only obtained from six RCTs (Additional file 4), no data were obtained from unpublished RCTs. This finding suggests that we might be missing data from unpublished trials. However, we contacted many trial authors for unpublished data and searched trial registries (for example, meta-Register) to identify potentially relevant unpublished RCTs. Furthermore, our funnel plots did not suggest that publication bias influenced our results [23].

A critical limitation, which may have influenced our results, is the determination of mismatch between circulating strains and those found in the vaccine. Characterizing strains as antigenically similar or distinct using HI assay or ferret antisera might be insensitive, leading to misclassification of strains [57]. Residual misclassification of matching due to the limited discriminatory ability of H1 assays may have also explained some of our findings, and is a limitation of the inferences that can be made. Furthermore, the cut-off values recommended by the CDC to distinguish between match and mismatch strains using the HI assay changed during the study period across the included RCTs, although most of the studies included here used a fourfold quotient HI cut-off (Table 1). As such, some of the data from trials labeled as matched might actually have been mismatched [58], and vice versa.

Conclusions

By summarizing the point estimates and confidence intervals for cross protection, these data can help public health officials anticipate the possible infection and complications during mismatched years. Estimates of protection for mismatched influenza seasons can be used by patients who are contemplating immunization, since the LAIV and TIV have been shown to offer benefit during matched seasons, as well as mismatched seasons.

Competing interests

ACT, DH, MT, CTB, and ML have received consulting fees from GlaxoSmithKline. CTB has received a grant from GlaxoSmithKline. ML received payment for medical writing. St Michael's Hospital received a grant to conduct this research. ACT and ML have received financial support for travel to meetings. AC and GM are paid employees of GlaxoSmithKline and also own company stock. CS has nothing to declare. This systematic review was funded by GlaxoSmithKline, Canada. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Authors’ contributions

ACT conceived the study, designed the study, screened citations and full text articles, abstracted the data, analyzed the data, interpreted the data, and wrote the manuscript. AC conceived the study and edited the manuscript. CS screened citations and full text articles, abstracted the data, generated tables, and edited the manuscript. DH screened citations and full text articles and edited the manuscript. GM conceived the study and edited the manuscript. MHC conducted the statistical analysis, interpreted the data and edited the manuscript. MK screened citations and full text articles and edited the manuscript. CB conceived the study and edited the manuscript. ML conceived the study, designed the study, interpreted the data and edited the manuscript. All authors have read and approved the final manuscript.

Pre-publication history

The pre-publication history for this paper can be accessed here:

http://www.biomedcentral.com/1741-7015/11/153/prepub

Additional file 1

Study characteristics.

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Additional file 2

Other methodological concerns.

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Additional file 3

Meta-analysis results.

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Additional file 4

Unpublished data from other authors.

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