OBJECTIVES: Published transmission electron microscopy analysis of in vitro resin-dentin bonds shows that, after 44 months, almost 70% of collagen fibrils from the hybrid layer disappear. Matrix metalloproteinases (MMPs) play an important role in that process and are thought to be the main factor responsible for the solubilization of dentin collagen. Therefore, this study aimed to evaluate the inactivation of matrix-bound MMPs by two different cross-linking agents, carbodiimide (EDC) or proanthocyanidin (PA), or the MMP-inhibitor, chlorhexidine (CHX), on acid-etched dentin using a simplified MMP assay method. MATERIALS AND METHODS: Dentin beams (2×1×6 mm) were obtained from mid-coronal dentin of sound third molars and randomly divided into six groups (G) according to the dentin treatment: G1: Deionized water (control); G2: 0.1 M EDC; G3: 0.5 M EDC; G4: 0.5 M EDC + 35% hydroxyethyl methacrylate (HEMA); G5: 5% PA; and G6: 2% CHX. The beams were etched for 15 seconds with 37% phosphoric acid, rinsed, and then immersed for 60 seconds in one of the treatment solutions. The data were expressed both in absorbance values at 412 nm and in MMP-9 activity equivalents. The total MMP activity of dentin was analyzed for one hour by colorimetric assay (Sensolyte). Data were submitted to Wilcoxon nonparametric test and Mann-Whitney tests (p>0.05). RESULTS: All experimental cross-linking solutions significantly reduced MMP activity from 79.8% to 95.2% when compared to the control group. No difference was observed among 0.1 M EDC (84.8%), 5% PA (87.6%), and 2% CHX (79.8%). Addition of 35% HEMA to 0.5 M EDC produced inactivation (95.2%) that was similar to that of 0.5 M EDC alone (92.7%). CONCLUSION: Dentin treatment with cross-linking agents is effective to significantly reduce MMP activity. Mixing 0.5 M EDC and 35% HEMA did not influence EDC inhibitor potential.
RCT Entities:
OBJECTIVES: Published transmission electron microscopy analysis of in vitro resin-dentin bonds shows that, after 44 months, almost 70% of collagen fibrils from the hybrid layer disappear. Matrix metalloproteinases (MMPs) play an important role in that process and are thought to be the main factor responsible for the solubilization of dentin collagen. Therefore, this study aimed to evaluate the inactivation of matrix-bound MMPs by two different cross-linking agents, carbodiimide (EDC) or proanthocyanidin (PA), or the MMP-inhibitor, chlorhexidine (CHX), on acid-etched dentin using a simplified MMP assay method. MATERIALS AND METHODS: Dentin beams (2×1×6 mm) were obtained from mid-coronal dentin of sound third molars and randomly divided into six groups (G) according to the dentin treatment: G1: Deionized water (control); G2: 0.1 M EDC; G3: 0.5 M EDC; G4: 0.5 M EDC + 35% hydroxyethyl methacrylate (HEMA); G5: 5% PA; and G6: 2% CHX. The beams were etched for 15 seconds with 37% phosphoric acid, rinsed, and then immersed for 60 seconds in one of the treatment solutions. The data were expressed both in absorbance values at 412 nm and in MMP-9 activity equivalents. The total MMP activity of dentin was analyzed for one hour by colorimetric assay (Sensolyte). Data were submitted to Wilcoxon nonparametric test and Mann-Whitney tests (p>0.05). RESULTS: All experimental cross-linking solutions significantly reduced MMP activity from 79.8% to 95.2% when compared to the control group. No difference was observed among 0.1 M EDC (84.8%), 5% PA (87.6%), and 2% CHX (79.8%). Addition of 35% HEMA to 0.5 M EDC produced inactivation (95.2%) that was similar to that of 0.5 M EDC alone (92.7%). CONCLUSION: Dentin treatment with cross-linking agents is effective to significantly reduce MMP activity. Mixing 0.5 M EDC and 35% HEMA did not influence EDC inhibitor potential.
Authors: Arzu Tezvergil-Mutluay; Kelli A Agee; Tomohiro Hoshika; Franklin R Tay; David H Pashley Journal: Acta Biomater Date: 2010-05-23 Impact factor: 8.947
Authors: Ana Karina B Bedran-Russo; Cristina M P Vidal; Paulo H Dos Santos; Carina S Castellan Journal: J Biomed Mater Res B Appl Biomater Date: 2010-07 Impact factor: 3.368
Authors: P M C Scaffa; C M P Vidal; N Barros; T F Gesteira; A K Carmona; L Breschi; D H Pashley; L Tjäderhane; I L S Tersariol; F D Nascimento; M R Carrilho Journal: J Dent Res Date: 2012-01-19 Impact factor: 6.116
Authors: David H Pashley; Franklin R Tay; Lorenzo Breschi; Leo Tjäderhane; Ricardo M Carvalho; Marcela Carrilho; Arzu Tezvergil-Mutluay Journal: Dent Mater Date: 2010-11-27 Impact factor: 5.304
Authors: Hérica A Ricci; Mariane E Sanabe; Carlos A de Souza Costa; David H Pashley; Josimeri Hebling Journal: Eur J Oral Sci Date: 2010-08 Impact factor: 2.612
Authors: Jianfeng Zhou; Ayaka Chiba; Debora L S Scheffel; Josimeri Hebling; Kelli Agee; Junji Tagami; Jianquo Tan; Dalia Abuelenain; Manar Abu Nawareg; Ali H Hassan; Lorenzo Breschi; Franklin R Tay; David H Pashley Journal: Dent Mater Date: 2016-07-16 Impact factor: 5.304
Authors: Kelli A Agee; Anuradha Prakki; Tariq Abu-Haimed; Ghada H Naguib; Manar Abu Nawareg; Arzu Tezvergil-Mutluay; Debora L S Scheffel; Chen Chen; Seung Soon Jang; Hyea Hwang; Martha Brackett; Geneviéve Grégoire; Franklin R Tay; Lorenzo Breschi; David H Pashley Journal: Dent Mater Date: 2015-01-19 Impact factor: 5.304
Authors: D L S Scheffel; C C Delgado; D G Soares; F G Basso; C A de Souza Costa; D H Pashley; J Hebling Journal: Oper Dent Date: 2015-03-12 Impact factor: 2.440
Authors: Heonjune Ryou; Gianluca Turco; Lorenzo Breschi; Franklin R Tay; David H Pashley; Dwayne Arola Journal: Dent Mater Date: 2015-12-30 Impact factor: 5.304