Jianfeng Zhou1, Ayaka Chiba2, Debora L S Scheffel3, Josimeri Hebling3, Kelli Agee4, Junji Tagami2, Jianquo Tan1, Dalia Abuelenain5, Manar Abu Nawareg5, Ali H Hassan5, Lorenzo Breschi6, Franklin R Tay7, David H Pashley8. 1. Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing, PR China. 2. Department of Cariology and Operative Dentistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan. 3. Department of Orthodontics and Pediatric Dentistry, Universidade Estadual Paulista-UNESP, Araraquara Dental School, Araraquara, Sao Paulo, Brazil. 4. Department of Oral Biology, The Dental College of Georgia, Augusta University, Augusta, GA, USA. 5. Faculty of Dentistry, King Abdulaziz University, Jeddah, Saudi Arabia. 6. Department of Biomedical and Neuromotor Sciences DIBINEM, University of Bologna and IGM-CNR Unit of Bologna, Bologna, Italy. 7. Department of Endodontics, The Dental College of Georgia, Augusta University, Augusta, GA, USA. 8. Department of Oral Biology, The Dental College of Georgia, Augusta University, Augusta, GA, USA. Electronic address: DPASHLEY@gru.edu.
Abstract
OBJECTIVE: To determine if acid-etched, cross-linked dentin can be dehydrated without lowering bond strength below that of cross-linked wet-bonded dentin in vitro. METHODS: Using extracted human third molars, control acid-etched dentin was bonded with Single Bond Plus, using either the wet- or dry-bonding technique. Experimental acid-etched dentin was treated with 5mass% grape seed extract (GSE) in different solvents for 1min before undergoing wet vs dry resin-dentin bonding with Single Bond Plus. Completely demineralized dentin beams were treated with 5% GSE for 0, 1 or 10min, before measuring stiffness by 3-point flexure. Other completely demineralized beams were treated similarly and then incubated in buffer for 1 week to measure the collagen solubilization by endogenous dentin proteases. RESULTS: 24h microtensile bond strengths (μTBS) in wet and dry controls were 53.5±3.6 and 9.4±1.8MPa, respectively (p<0.05). 5% GSE in water gave μTBS of 53.7±3.4 and 39.1±9.7MPa (p<0.05), respectively, while 5% GSE in ethanol gave μTBS of 51.2±2.3 and 35.3±2.0MPa (p<0.05). 5% GSE in 5% EtOH/95% water gave wet and dry μTBS of 53.0±2.3 and 55.7±5.1MPa (p>0.05). Cross-linking demineralized dentin with 5% GSE increased stiffness of dentin and decreased collagen degradation (p<0.05). SIGNIFICANCE: 5% GSE pretreatment of acid-etched dentin for 1min permits the dentin to be completely air-dried without lowering bond strength.
OBJECTIVE: To determine if acid-etched, cross-linked dentin can be dehydrated without lowering bond strength below that of cross-linked wet-bonded dentin in vitro. METHODS: Using extracted human third molars, control acid-etched dentin was bonded with Single Bond Plus, using either the wet- or dry-bonding technique. Experimental acid-etched dentin was treated with 5mass% grape seed extract (GSE) in different solvents for 1min before undergoing wet vs dry resin-dentin bonding with Single Bond Plus. Completely demineralized dentin beams were treated with 5% GSE for 0, 1 or 10min, before measuring stiffness by 3-point flexure. Other completely demineralized beams were treated similarly and then incubated in buffer for 1 week to measure the collagen solubilization by endogenous dentin proteases. RESULTS: 24h microtensile bond strengths (μTBS) in wet and dry controls were 53.5±3.6 and 9.4±1.8MPa, respectively (p<0.05). 5% GSE in water gave μTBS of 53.7±3.4 and 39.1±9.7MPa (p<0.05), respectively, while 5% GSE in ethanol gave μTBS of 51.2±2.3 and 35.3±2.0MPa (p<0.05). 5% GSE in 5% EtOH/95% water gave wet and dry μTBS of 53.0±2.3 and 55.7±5.1MPa (p>0.05). Cross-linking demineralized dentin with 5% GSE increased stiffness of dentin and decreased collagen degradation (p<0.05). SIGNIFICANCE: 5% GSE pretreatment of acid-etched dentin for 1min permits the dentin to be completely air-dried without lowering bond strength.
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