OBJECTIVES: To characterize the interaction of 1-Ethyl-3-[3-dimethylaminopropyl] carbodiimide Hydrochloride (EDC) with dentin matrix and its effect on the resin-dentin bond. METHODS: Changes to the stiffness of demineralized dentin fragments treated with EDC/N-hydroxysuccinimide (NHS) in different solutions were evaluated at different time points. The resistance against enzymatic degradation was indirectly evaluated by ultimate tensile strength (UTS) test of demineralized dentin treated or not with EDC/NHS and subjected to collagenase digestion. Short- and long-term evaluations of the strength of resin-dentin interfaces treated with EDC/NHS for 1 h were performed using microtensile bond strength (microTBS) test. All data (MPa) were individually analyzed using ANOVA and Tukey HSD tests (alpha = 0.05). RESULTS: The different exposure times significantly increased the stiffness of dentin (p < 0.0001, control-5.15 and EDC/NHS-29.50), while no differences were observed among the different solutions of EDC/NHS (p = 0.063). Collagenase challenge did not affect the UTS values of EDC/NHS group (6.08) (p > 0.05), while complete degradation was observed for the control group (p = 0.0008, control-20.84 and EDC/NHS-43.15). EDC/NHS treatment did not significantly increase resin-dentin muTBS, but the values remained stable after 12 months water storage (p < 0.05). CONCLUSIONS: Biomimetic use of EDC/NHS to induce exogenous collagen cross-links resulted in increased mechanical properties and stability of dentin matrix and dentin-resin interfaces. (c) 2010 Wiley Periodicals, Inc.
OBJECTIVES: To characterize the interaction of 1-Ethyl-3-[3-dimethylaminopropyl] carbodiimide Hydrochloride (EDC) with dentin matrix and its effect on the resin-dentin bond. METHODS: Changes to the stiffness of demineralized dentin fragments treated with EDC/N-hydroxysuccinimide (NHS) in different solutions were evaluated at different time points. The resistance against enzymatic degradation was indirectly evaluated by ultimate tensile strength (UTS) test of demineralized dentin treated or not with EDC/NHS and subjected to collagenase digestion. Short- and long-term evaluations of the strength of resin-dentin interfaces treated with EDC/NHS for 1 h were performed using microtensile bond strength (microTBS) test. All data (MPa) were individually analyzed using ANOVA and Tukey HSD tests (alpha = 0.05). RESULTS: The different exposure times significantly increased the stiffness of dentin (p < 0.0001, control-5.15 and EDC/NHS-29.50), while no differences were observed among the different solutions of EDC/NHS (p = 0.063). Collagenase challenge did not affect the UTS values of EDC/NHS group (6.08) (p > 0.05), while complete degradation was observed for the control group (p = 0.0008, control-20.84 and EDC/NHS-43.15). EDC/NHS treatment did not significantly increase resin-dentin muTBS, but the values remained stable after 12 months water storage (p < 0.05). CONCLUSIONS: Biomimetic use of EDC/NHS to induce exogenous collagen cross-links resulted in increased mechanical properties and stability of dentin matrix and dentin-resin interfaces. (c) 2010 Wiley Periodicals, Inc.
Authors: Raquel Osorio; Jatyr Pisani-Proenca; Maria Carolina G Erhardt; Estrella Osorio; Fátima S Aguilera; Franklin R Tay; Manuel Toledano Journal: J Dent Date: 2008-02 Impact factor: 4.379
Authors: Ana Karina B Bedran-Russo; David H Pashley; Kelli Agee; James L Drummond; Klaus J Miescke Journal: J Biomed Mater Res B Appl Biomater Date: 2008-08 Impact factor: 3.368
Authors: Leo Tjäderhane; Fabio D Nascimento; Lorenzo Breschi; Annalisa Mazzoni; Ivarne L S Tersariol; Saulo Geraldeli; Arzu Tezvergil-Mutluay; Marcela Carrilho; Ricardo M Carvalho; Franklin R Tay; David H Pashley Journal: Dent Mater Date: 2013-08-14 Impact factor: 5.304
Authors: G E Kim; A A Leme-Kraus; R Phansalkar; G Viana; C Wu; S-N Chen; G F Pauli; Akb Bedran-Russo Journal: Oper Dent Date: 2016-11-28 Impact factor: 2.440
Authors: A Tezvergil-Mutluay; M M Mutluay; K A Agee; R Seseogullari-Dirihan; T Hoshika; M Cadenaro; L Breschi; P Vallittu; F R Tay; D H Pashley Journal: J Dent Res Date: 2011-11-04 Impact factor: 6.116
Authors: Zihou Zhang; Dylan Beitzel; Hessam Majd; Mustafa Mutluay; Arzu Tezvergil-Mutluay; Franklin R Tay; David H Pashley; Dwayne Arola Journal: Dent Mater Date: 2017-09-21 Impact factor: 5.304
Authors: D L S Scheffel; C C Delgado; D G Soares; F G Basso; C A de Souza Costa; D H Pashley; J Hebling Journal: Oper Dent Date: 2015-03-12 Impact factor: 2.440
Authors: Ana Beatriz Silva Sousa; Cristina M P Vidal; Ariene Arcas Leme-Kraus; Fernanda C P Pires-de-Souza; Ana K Bedran-Russo Journal: Dent Mater Date: 2016-08-11 Impact factor: 5.304
Authors: Zihou Zhang; Dylan Beitzel; Hessam Majd; Mustafa Mutluay; Arzu Tezvergil-Mutluay; Franklin R Tay; David H Pashley; Dwayne Arola Journal: Dent Mater Date: 2015-12-29 Impact factor: 5.304