Yi Liu1, Xinyan Bai1, Shaohua Li1, Ying Liu1, Andrew Keightley2, Yong Wang3. 1. School of Dentistry, University of Missouri-Kansas City, Kansas City, MO 64108, USA. 2. School of Biological Sciences, University of Missouri-Kansas City, Kansas City, MO 64110, USA. 3. School of Dentistry, University of Missouri-Kansas City, Kansas City, MO 64108, USA. Electronic address: Wangyo@umkc.edu.
Abstract
OBJECTIVE: To investigate the relationship between the structures of polyphenolic compounds found in grape seed extract (GSE) and their activity in cross-linking dentin collagen in clinically relevant settings. METHODS: Representative monomeric and dimeric GSE constituents including (+)-catechin (pCT), (-)-catechin (CT), (-)-epicatechin (EC), (-)-epigallocatechin (EGC), (-)-epicatechin gallate (ECG), (-)-epigallocatechin gallate (EGCG), procyanidin B2 and a pCT-pCT dimer were purchased or synthesized. GSE was separated into low (PALM) and high molecular weight (PAHM) fractions. Human molars were processed into dentin films and beams. After demineralization, 11 groups of films (n=5) were treated for 1min with the aforementioned reagents (1wt% in 50/50 ethanol/water) and 1 group remained untreated. The films were studied by Fourier transform infrared spectroscopy (FTIR) followed by a quantitative mass spectroscopy-based digestion assay. Tensile properties of demineralized dentin beams were evaluated (n=7) after treatments (2h and 24h) with selective GSE species that were found to protect dentin collagen from collagenase. RESULTS: Efficacy of GSE constituents in cross-linking dentin collagen was dependent on molecular size and galloylation. Non-galloylated species with degree of polymerization up to two, including pCT, CT, EC, EGC, procyanidin B2 and pCT-pCT dimer were not active. Galloylated species were active starting from monomeric form, including ECG, EGCG, PALM, GSE and PAHM. PALM induced the best overall improvement in tensile properties of dentin collagen. SIGNIFICANCE: Identification under clinically relevant settings of structural features that contribute to GSE constituents' efficacy in stabilizing demineralized dentin matrix has immediate impact on optimizing GSE's use in dentin bonding.
OBJECTIVE: To investigate the relationship between the structures of polyphenolic compounds found in grape seed extract (GSE) and their activity in cross-linking dentin collagen in clinically relevant settings. METHODS: Representative monomeric and dimeric GSE constituents including (+)-catechin (pCT), (-)-catechin (CT), (-)-epicatechin (EC), (-)-epigallocatechin (EGC), (-)-epicatechin gallate (ECG), (-)-epigallocatechin gallate (EGCG), procyanidin B2 and a pCT-pCT dimer were purchased or synthesized. GSE was separated into low (PALM) and high molecular weight (PAHM) fractions. Human molars were processed into dentin films and beams. After demineralization, 11 groups of films (n=5) were treated for 1min with the aforementioned reagents (1wt% in 50/50 ethanol/water) and 1 group remained untreated. The films were studied by Fourier transform infrared spectroscopy (FTIR) followed by a quantitative mass spectroscopy-based digestion assay. Tensile properties of demineralized dentin beams were evaluated (n=7) after treatments (2h and 24h) with selective GSE species that were found to protect dentin collagen from collagenase. RESULTS: Efficacy of GSE constituents in cross-linking dentin collagen was dependent on molecular size and galloylation. Non-galloylated species with degree of polymerization up to two, including pCT, CT, EC, EGC, procyanidin B2 and pCT-pCT dimer were not active. Galloylated species were active starting from monomeric form, including ECG, EGCG, PALM, GSE and PAHM. PALM induced the best overall improvement in tensile properties of dentin collagen. SIGNIFICANCE: Identification under clinically relevant settings of structural features that contribute to GSE constituents' efficacy in stabilizing demineralized dentin matrix has immediate impact on optimizing GSE's use in dentin bonding.
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