Literature DB >> 23442004

Orthogonal spin labeling and Gd(III)-nitroxide distance measurements on bacteriophage T4-lysozyme.

Luca Garbuio1, Enrica Bordignon, Evan K Brooks, Wayne L Hubbell, Gunnar Jeschke, Maxim Yulikov.   

Abstract

We present the first example of chemoselective site-specific spin labeling of a monomeric protein with two spectroscopically orthogonal spin labels: a gadolinium(III) chelate complex and a nitroxide radical. A detailed analysis of the performance of two commercially available Gd(III) ligands in the Gd(III)-nitroxide pulse double electron-electron resonance (DEER or PELDOR) experiment is reported. A modification of the flip angle of the pump pulse in the Gd(III)-nitroxide DEER experiment is proposed to optimize sensitivity.

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Year:  2013        PMID： 23442004      PMCID： PMC3634801          DOI： 10.1021/jp401806g

Source DB:  PubMed          Journal:  J Phys Chem B        ISSN： 1520-5207            Impact factor:   2.991


  32 in total

1.  Dead-time free measurement of dipole-dipole interactions between electron spins.

Authors:  M Pannier; S Veit; A Godt; G Jeschke; H W Spiess
Journal:  J Magn Reson       Date:  2000-02       Impact factor: 2.229

2.  Selective measurements of a nitroxide-nitroxide separation of 5 nm and a nitroxide-copper separation of 2.5 nm in a terpyridine-based copper(II) complex by pulse EPR spectroscopy.

Authors:  Evelyn Narr; Adelheid Godt; Gunnar Jeschke
Journal:  Angew Chem Int Ed Engl       Date:  2002-10-18       Impact factor: 15.336

3.  The determination of pair distance distributions by pulsed ESR using Tikhonov regularization.

Authors:  Yun-Wei Chiang; Peter P Borbat; Jack H Freed
Journal:  J Magn Reson       Date:  2005-02       Impact factor: 2.229

4.  Measurement of a Cu-Cu distance of 26 A by a pulsed EPR method.

Authors:  Irene M C van Amsterdam; Marcellus Ubbink; Gerard W Canters; Martina Huber
Journal:  Angew Chem Int Ed Engl       Date:  2003-01-03       Impact factor: 15.336

5.  An approach towards the measurement of nanometer range distances based on Cu2+ ions and ESR.

Authors:  Zhongyu Yang; Drew Kise; Sunil Saxena
Journal:  J Phys Chem B       Date:  2010-05-13       Impact factor: 2.991

6.  Multiple Pathway Relaxation Enhancement in the System Composed of Three Paramagnetic Species: Nitroxide Radical-Ln(3+)-O2.

Authors:  Petra Lueders; Heidrun Jäger; Marcus A Hemminga; Gunnar Jeschke; Maxim Yulikov
Journal:  J Phys Chem Lett       Date:  2012-05-04       Impact factor: 6.475

7.  Determining α-helical and β-sheet secondary structures via pulsed electron spin resonance spectroscopy.

Authors:  Andy Zhou; Shadi Abu-Baker; Indra D Sahu; Lishan Liu; Robert M McCarrick; Carole Dabney-Smith; Gary A Lorigan
Journal:  Biochemistry       Date:  2012-09-14       Impact factor: 3.162

8.  Isotope selection in distance measurements between nitroxides.

Authors:  G Jeschke; H Zimmermann; A Godt
Journal:  J Magn Reson       Date:  2006-02-21       Impact factor: 2.229

9.  PELDOR measurements on a nitroxide-labeled Cu(II) porphyrin: orientation selection, spin-density distribution, and conformational flexibility.

Authors:  Bela E Bode; Jörn Plackmeyer; Thomas F Prisner; Olav Schiemann
Journal:  J Phys Chem A       Date:  2008-05-20       Impact factor: 2.781

Review 10.  Long-range distance determinations in biomacromolecules by EPR spectroscopy.

Authors:  Olav Schiemann; Thomas F Prisner
Journal:  Q Rev Biophys       Date:  2007-06-13       Impact factor: 5.318
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  15 in total

1.  Reaching the quantum limit of sensitivity in electron spin resonance.

Authors:  A Bienfait; J J Pla; Y Kubo; M Stern; X Zhou; C C Lo; C D Weis; T Schenkel; M L W Thewalt; D Vion; D Esteve; B Julsgaard; K Mølmer; J J L Morton; P Bertet
Journal:  Nat Nanotechnol       Date:  2015-12-14       Impact factor: 39.213

2.  Gd3+-Gd3+ distances exceeding 3 nm determined by very high frequency continuous wave electron paramagnetic resonance.

Authors:  Jessica A Clayton; Mian Qi; Adelheid Godt; Daniella Goldfarb; Songi Han; Mark S Sherwin
Journal:  Phys Chem Chem Phys       Date:  2017-02-15       Impact factor: 3.676

3.  Copper-based pulsed dipolar ESR spectroscopy as a probe of protein conformation linked to disease states.

Authors:  Gregory E Merz; Peter P Borbat; Ashley J Pratt; Elizabeth D Getzoff; Jack H Freed; Brian R Crane
Journal:  Biophys J       Date:  2014-10-07       Impact factor: 4.033

Review 4.  Technological advances in site-directed spin labeling of proteins.

Authors:  Wayne L Hubbell; Carlos J López; Christian Altenbach; Zhongyu Yang
Journal:  Curr Opin Struct Biol       Date:  2013-07-11       Impact factor: 6.809

5.  Zero field splitting fluctuations induced phase relaxation of Gd3+ in frozen solutions at cryogenic temperatures.

Authors:  A Raitsimring; A Dalaloyan; A Collauto; A Feintuch; T Meade; D Goldfarb
Journal:  J Magn Reson       Date:  2014-09-30       Impact factor: 2.229

6.  Conformational cycle of the vitamin B12 ABC importer in liposomes detected by double electron-electron resonance (DEER).

Authors:  Benesh Joseph; Vladimir M Korkhov; Maxim Yulikov; Gunnar Jeschke; Enrica Bordignon
Journal:  J Biol Chem       Date:  2013-12-19       Impact factor: 5.157

7.  EPR relaxation-enhancement-based distance measurements on orthogonally spin-labeled T4-lysozyme.

Authors:  Sahand Razzaghi; Evan K Brooks; Enrica Bordignon; Wayne L Hubbell; Maxim Yulikov; Gunnar Jeschke
Journal:  Chembiochem       Date:  2013-06-14       Impact factor: 3.164

8.  Adapter reagents for protein site specific dye labeling.

Authors:  Darren A Thompson; Eric G B Evans; Tomas Kasza; Glenn L Millhauser; Philip E Dawson
Journal:  Biopolymers       Date:  2014-05       Impact factor: 2.505

9.  Spin-labeled nanobodies as protein conformational reporters for electron paramagnetic resonance in cellular membranes.

Authors:  Laura Galazzo; Gianmarco Meier; M Hadi Timachi; Cedric A J Hutter; Markus A Seeger; Enrica Bordignon
Journal:  Proc Natl Acad Sci U S A       Date:  2020-01-21       Impact factor: 11.205

10.  Encoded loop-lanthanide-binding tags for long-range distance measurements in proteins by NMR and EPR spectroscopy.

Authors:  Dominic Barthelmes; Markus Gränz; Katja Barthelmes; Karen N Allen; Barbara Imperiali; Thomas Prisner; Harald Schwalbe
Journal:  J Biomol NMR       Date:  2015-09-04       Impact factor: 2.835
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