Literature DB >> 22966895

Determining α-helical and β-sheet secondary structures via pulsed electron spin resonance spectroscopy.

Andy Zhou1, Shadi Abu-Baker, Indra D Sahu, Lishan Liu, Robert M McCarrick, Carole Dabney-Smith, Gary A Lorigan.   

Abstract

A new method has been developed to determine α-helical and β-sheet secondary structural components of aqueous and membrane-bound proteins using pulsed electron paramagnetic resonance (EPR) spectroscopy. The three-pulse electron spin echo envelope modulation (ESEEM) technique was used to detect weakly coupled (2)H-labeled nuclei on side chains in the proximity of a strategically placed nitroxide spin-label up to 8 Å away. Changes in the ESEEM spectra for different samples correlate directly to periodic structural differences between α-helical and β-sheet motifs. These distinct trends were demonstrated with α-helical (M2δ subunit of the acetylcholine receptor) and β-sheet (ubiquitin) peptides in biologically relevant sample environments.

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Year:  2012        PMID： 22966895      PMCID： PMC3535504          DOI： 10.1021/bi3010736

Source DB:  PubMed          Journal:  Biochemistry        ISSN： 0006-2960            Impact factor:   3.162


  23 in total

1.  Challenging the limit: NMR assignment of a 31 kDa helical membrane protein.

Authors:  Chengdong Huang; Smita Mohanty
Journal:  J Am Chem Soc       Date:  2010-03-24       Impact factor: 15.419

2.  A structure-based simulation approach for electron paramagnetic resonance spectra using molecular and stochastic dynamics simulations.

Authors:  Christian Beier; Heinz-Jürgen Steinhoff
Journal:  Biophys J       Date:  2006-07-14       Impact factor: 4.033

3.  Methods and applications of site-directed spin labeling EPR spectroscopy.

Authors:  Candice S Klug; Jimmy B Feix
Journal:  Methods Cell Biol       Date:  2008       Impact factor: 1.441

4.  De novo high-resolution protein structure determination from sparse spin-labeling EPR data.

Authors:  Nathan Alexander; Marco Bortolus; Ahmad Al-Mestarihi; Hassane Mchaourab; Jens Meiler
Journal:  Structure       Date:  2008-02       Impact factor: 5.006

Review 5.  Alpha-helical transmembrane peptides: a "divide and conquer" approach to membrane proteins.

Authors:  Natalie Bordag; Sandro Keller
Journal:  Chem Phys Lipids       Date:  2010-01       Impact factor: 3.329

Review 6.  Crystal structures of all-alpha type membrane proteins.

Authors:  Karen McLuskey; Aleksander W Roszak; Yanshi Zhu; Neil W Isaacs
Journal:  Eur Biophys J       Date:  2009-10-14       Impact factor: 1.733

7.  Solid-state (2)H and (15)N NMR studies of side-chain and backbone dynamics of phospholamban in lipid bilayers: investigation of the N27A mutation.

Authors:  Shidong Chu; Aaron T Coey; Gary A Lorigan
Journal:  Biochim Biophys Acta       Date:  2009-10-17

8.  Determining the topology of integral membrane peptides using EPR spectroscopy.

Authors:  Johnson J Inbaraj; Thomas B Cardon; Mikhail Laryukhin; Stuart M Grosser; Gary A Lorigan
Journal:  J Am Chem Soc       Date:  2006-07-26       Impact factor: 15.419

9.  Structural constraints on the transmembrane and juxtamembrane regions of the phospholamban pentamer in membrane bilayers: Gln29 and Leu52.

Authors:  Wei Liu; Jeffrey Z Fei; Toru Kawakami; Steven O Smith
Journal:  Biochim Biophys Acta       Date:  2007-10-22

10.  Parametrization, molecular dynamics simulation, and calculation of electron spin resonance spectra of a nitroxide spin label on a polyalanine alpha-helix.

Authors:  Deniz Sezer; Jack H Freed; Benoît Roux
Journal:  J Phys Chem B       Date:  2008-04-16       Impact factor: 2.991
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  8 in total

1.  Development of electron spin echo envelope modulation spectroscopy to probe the secondary structure of recombinant membrane proteins in a lipid bilayer.

Authors:  Rongfu Zhang; Indra D Sahu; Kaylee R Gibson; Nefertiti B Muhammad; Avnika P Bali; Raven G Comer; Lishan Liu; Andrew F Craig; Robert M Mccarrick; Carole Dabney-Smith; Charles R Sanders; Gary A Lorigan
Journal:  Protein Sci       Date:  2015-09-09       Impact factor: 6.725

2.  Determining the Secondary Structure of Membrane Proteins and Peptides Via Electron Spin Echo Envelope Modulation (ESEEM) Spectroscopy.

Authors:  Lishan Liu; Daniel J Mayo; Indra D Sahu; Andy Zhou; Rongfu Zhang; Robert M McCarrick; Gary A Lorigan
Journal:  Methods Enzymol       Date:  2015-08-01       Impact factor: 1.600

3.  Orthogonal spin labeling and Gd(III)-nitroxide distance measurements on bacteriophage T4-lysozyme.

Authors:  Luca Garbuio; Enrica Bordignon; Evan K Brooks; Wayne L Hubbell; Gunnar Jeschke; Maxim Yulikov
Journal:  J Phys Chem B       Date:  2013-03-08       Impact factor: 2.991

4.  Probing the Secondary Structure of Membrane Peptides Using (2)H-Labeled d(10)-Leucine via Site-Directed Spin-Labeling and Electron Spin Echo Envelope Modulation Spectroscopy.

Authors:  Lishan Liu; Indra D Sahu; Robert M McCarrick; Gary A Lorigan
Journal:  J Phys Chem B       Date:  2016-01-20       Impact factor: 2.991

Review 5.  Use of electron paramagnetic resonance to solve biochemical problems.

Authors:  Indra D Sahu; Robert M McCarrick; Gary A Lorigan
Journal:  Biochemistry       Date:  2013-08-20       Impact factor: 3.162

6.  Utilization of 13C-labeled amino acids to probe the α-helical local secondary structure of a membrane peptide using electron spin echo envelope modulation (ESEEM) spectroscopy.

Authors:  Lauren Bottorf; Indra D Sahu; Robert M McCarrick; Gary A Lorigan
Journal:  Biochim Biophys Acta Biomembr       Date:  2018-04-22       Impact factor: 3.747

Review 7.  Site-Directed Spin Labeling EPR for Studying Membrane Proteins.

Authors:  Indra D Sahu; Gary A Lorigan
Journal:  Biomed Res Int       Date:  2018-01-23       Impact factor: 3.411

Review 8.  Electron Paramagnetic Resonance as a Tool for Studying Membrane Proteins.

Authors:  Indra D Sahu; Gary A Lorigan
Journal:  Biomolecules       Date:  2020-05-13
  8 in total

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