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Literature DB >> 23368695

Engineering fungal nonreducing polyketide synthase by heterologous expression and domain swapping.

Hsu-Hua Yeh¹, Shu-Lin Chang, Yi-Ming Chiang, Kenneth S Bruno, Berl R Oakley, Tung-Kung Wu, Clay C C Wang.

Abstract

We reannotated the A. niger NR-PKS gene, e_gw1_19.204, and its downstream R domain gene, est_GWPlus_C_190476, as a single gene which we named dtbA. Heterologous expression of dtbA in A. nidulans demonstrated that DtbA protein produces two polyketides, 2,4-dihydroxy-3,5,6-trimethylbenzaldehyde (1) and 6-ethyl-2,4-dihydroxy-3,5-dimethylbenzaldehyde (2). Generation of DtbAΔR+TE chimeric PKSs by swapping the DtbA R domain with the AusA (austinol biosynthesis) or ANID_06448 TE domain enabled the production of two metabolites with carboxylic acids replacing the corresponding aldehydes.

Entities: Chemical Mutation Species

Mesh：

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Year: 2013 PMID： 23368695 PMCID： PMC3681297 DOI： 10.1021/ol303328t

Source DB: PubMed Journal: Org Lett ISSN： 1523-7052 Impact factor: 6.005

21 in total

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5. Exploring Fungal Polyketide C-Methylation through Combinatorial Domain Swaps.

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Review 10. Steps towards the synthetic biology of polyketide biosynthesis.

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