BACKGROUND: Sample stability is critical for accurate analysis of drug compounds in biosamples. The use of additives to eradicate the enzymatic activity causing loss of these analytes has its limitations. RESULTS: A novel technique for sample stabilization by rapid, high-temperature heating was used. The stability of six commercial drugs in blood and blood spots was investigated under various conditions with or without heat stabilization at 95°C. Oseltamivir, cefotaxime and ribavirin were successfully stabilized by heating whereas significant losses were seen in unheated samples. Amodiaquine was stable with and without heating. Artemether and dihydroartemisinin were found to be very heat sensitive and began to decompose even at 60°C. CONCLUSION: Heat stabilization is a viable technique to maintain analytes in blood spot samples, without the use of chemical additives, by stopping the enzymatic activity that causes sample degradation.
BACKGROUND: Sample stability is critical for accurate analysis of drug compounds in biosamples. The use of additives to eradicate the enzymatic activity causing loss of these analytes has its limitations. RESULTS: A novel technique for sample stabilization by rapid, high-temperature heating was used. The stability of six commercial drugs in blood and blood spots was investigated under various conditions with or without heat stabilization at 95°C. Oseltamivir, cefotaxime and ribavirin were successfully stabilized by heating whereas significant losses were seen in unheated samples. Amodiaquine was stable with and without heating. Artemether and dihydroartemisinin were found to be very heat sensitive and began to decompose even at 60°C. CONCLUSION: Heat stabilization is a viable technique to maintain analytes in blood spot samples, without the use of chemical additives, by stopping the enzymatic activity that causes sample degradation.
Authors: Marcus Svensson; Mats Boren; Karl Sköld; Maria Fälth; Benita Sjögren; Malin Andersson; Per Svenningsson; Per E Andren Journal: J Proteome Res Date: 2009-02 Impact factor: 4.466
Authors: Gary B Smejkal; Chiara Rivas-Morello; Jae-Hyung Robert Chang; Emily Freeman; Alexander J Trachtenberg; Alexander Lazarev; Alexander R Ivanov; Winston P Kuo Journal: Electrophoresis Date: 2011-07-27 Impact factor: 3.535
Authors: Naiyu Zheng; Eliza N Fung; Adela Buzescu; Mark E Arnold; Jianing Zeng Journal: Rapid Commun Mass Spectrom Date: 2012-06-15 Impact factor: 2.419
Authors: Niklas Lindegardh; Geraint R Davies; Tran Tinh Hien; Jeremy Farrar; Pratap Singhasivanon; Nicholas P J Day; Nicholas J White Journal: Antimicrob Agents Chemother Date: 2007-02-26 Impact factor: 5.191
Authors: N Lindegårdh; W Hanpithakpong; Y Wattanagoon; P Singhasivanon; N J White; N P J Day Journal: J Chromatogr B Analyt Technol Biomed Life Sci Date: 2007-09-19 Impact factor: 3.205
Authors: Gero P Hooff; Roland J W Meesters; Jeroen J A van Kampen; Nick A van Huizen; Birgit Koch; Asmar F Y Al Hadithy; Teun van Gelder; Albert D M E Osterhaus; Rob A Gruters; Theo M Luider Journal: Anal Bioanal Chem Date: 2011-05-03 Impact factor: 4.142
Authors: Julie Autmizguine; Daniel K Benjamin; P Brian Smith; Mario Sampson; Philippe Ovetchkine; Michael Cohen-Wolkowiez; Kevin M Watt Journal: Curr Clin Pharmacol Date: 2014
Authors: Benjamin S Frey; Deidre E Damon; Danyelle M Allen; Jill Baker; Samuel Asamoah; Abraham K Badu-Tawiah Journal: Analyst Date: 2021-11-08 Impact factor: 4.616
Authors: Anu Marahatta; Vandana Megaraj; Patrick T McGann; Russell E Ware; Kenneth D R Setchell Journal: Clin Chem Date: 2016-09-30 Impact factor: 8.327
Authors: Barbara Adamczyk; Chunsheng Jin; Karol Polom; Pedro Muñoz; Miguel A Rojas-Macias; David Zeeberg; Mats Borén; Franco Roviello; Niclas G Karlsson Journal: Sci Rep Date: 2018-01-10 Impact factor: 4.379