BACKGROUND: Investigations have been undertaken of the effects of storage on the utility of using dried blood spots, collected and stored on a paper substrate, combined with reversed-phase ultra-performance LC (UPLC), electrospray ionization orthogonal acceleration ToF-MS (UPLC-oaToF-MS), as a method for performing global metabolic profiling studies. In addition, a range of products used to collect these blood spots have also been studied for effects on the UPLC-MS background. RESULTS: These preliminary data suggest that sample stability in this application is limited unless the cards are stored at, at least, -20°C and preferably at -80°C. In addition, the use of untreated cards is recommended to minimize incurred background interferences. CONCLUSION: Further investigations are necessary to improve the utility of this methodology in global metabolite profiling.
BACKGROUND: Investigations have been undertaken of the effects of storage on the utility of using dried blood spots, collected and stored on a paper substrate, combined with reversed-phase ultra-performance LC (UPLC), electrospray ionization orthogonal acceleration ToF-MS (UPLC-oaToF-MS), as a method for performing global metabolic profiling studies. In addition, a range of products used to collect these blood spots have also been studied for effects on the UPLC-MS background. RESULTS: These preliminary data suggest that sample stability in this application is limited unless the cards are stored at, at least, -20°C and preferably at -80°C. In addition, the use of untreated cards is recommended to minimize incurred background interferences. CONCLUSION: Further investigations are necessary to improve the utility of this methodology in global metabolite profiling.
Authors: David Allaway; Janet E Alexander; Laura J Carvell-Miller; Rhiannon M Reynolds; Catherine L Winder; Ralf J M Weber; Gavin R Lloyd; Andrew D Southam; Warwick B Dunn Journal: Front Vet Sci Date: 2022-06-22
Authors: Daniel Blessborn; Karl Sköld; David Zeeberg; Karnrawee Kaewkhao; Olof Sköld; Martin Ahnoff Journal: Bioanalysis Date: 2013-01 Impact factor: 2.681
Authors: Chiara Volani; Giulia Caprioli; Giovanni Calderisi; Baldur B Sigurdsson; Johannes Rainer; Ivo Gentilini; Andrew A Hicks; Peter P Pramstaller; Guenter Weiss; Sigurdur V Smarason; Giuseppe Paglia Journal: Anal Bioanal Chem Date: 2017-08-17 Impact factor: 4.142