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Literature DB >> 22997015

Genetically encoded cyclopropene directs rapid, photoclick-chemistry-mediated protein labeling in mammalian cells.

Zhipeng Yu¹, Yanchao Pan, Zhiyong Wang, Jiangyun Wang, Qing Lin.

Abstract

We just click: Genetic incorporation of a cyclopropene amino acid CpK (see scheme) site-specifically into proteins in E. coli and mammalian cells was achieved using an orthogonal aminoacyl-tRNA synthetase/tRNA(CUA) pair (CpKRS/MbtRNA(CUA)). Cyclopropene exhibited fast reaction kinetics in the photoclick reaction and allowed rapid (ca. 2 min) labeling of proteins.

Entities: CellLine Chemical Disease Gene Mutation Species

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Year: 2012 PMID： 22997015 PMCID： PMC3517012 DOI： 10.1002/anie.201205352

Source DB: PubMed Journal: Angew Chem Int Ed Engl ISSN： 1433-7851 Impact factor: 15.336

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