Literature DB >> 28902227

A genetically encoded cyclobutene probe for labelling of live cells.

K Liu1, B Enns, B Evans, N Wang, X Shang, W Sittiwong, P H Dussault, J Guo.   

Abstract

We have identified an aminoacyl-tRNA synthetase/tRNA pair for the efficient and site-specific incorporation of a cyclobutene-containing amino acid into proteins in response to an amber nonsense codon. Fast and fluorescent labeling of purified proteins and intact proteins in live cells was demonstrated using the inverse electron demand Diels-Alder reaction with a tetrazine.

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Year:  2017        PMID： 28902227      PMCID： PMC5648060          DOI： 10.1039/c7cc05580c

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  39 in total

1.  A facile system for encoding unnatural amino acids in mammalian cells.

Authors:  Peng R Chen; Dan Groff; Jiantao Guo; Weijia Ou; Susan Cellitti; Bernhard H Geierstanger; Peter G Schultz
Journal:  Angew Chem Int Ed Engl       Date:  2009       Impact factor: 15.336

Review 2.  Finding the right (bioorthogonal) chemistry.

Authors:  David M Patterson; Lidia A Nazarova; Jennifer A Prescher
Journal:  ACS Chem Biol       Date:  2014-01-30       Impact factor: 5.100

3.  Live-cell imaging of cyclopropene tags with fluorogenic tetrazine cycloadditions.

Authors:  Jun Yang; Jolita Šečkutė; Christian M Cole; Neal K Devaraj
Journal:  Angew Chem Int Ed Engl       Date:  2012-06-13       Impact factor: 15.336

4.  Tetrazine-based cycloadditions: application to pretargeted live cell imaging.

Authors:  Neal K Devaraj; Ralph Weissleder; Scott A Hilderbrand
Journal:  Bioconjug Chem       Date:  2008-12       Impact factor: 4.774

5.  Functionalized cyclopropenes as bioorthogonal chemical reporters.

Authors:  David M Patterson; Lidia A Nazarova; Bryan Xie; David N Kamber; Jennifer A Prescher
Journal:  J Am Chem Soc       Date:  2012-11-01       Impact factor: 15.419

6.  An expanded genetic code in mammalian cells with a functional quadruplet codon.

Authors:  Wei Niu; Peter G Schultz; Jiantao Guo
Journal:  ACS Chem Biol       Date:  2013-05-20       Impact factor: 5.100

7.  A photochemical synthesis of functionalized trans-cyclooctenes driven by metal complexation.

Authors:  Maksim Royzen; Glenn P A Yap; Joseph M Fox
Journal:  J Am Chem Soc       Date:  2008-03-06       Impact factor: 15.419

8.  Bioorthogonal reactions for labeling proteins.

Authors:  Kathrin Lang; Jason W Chin
Journal:  ACS Chem Biol       Date:  2014-01-17       Impact factor: 5.100

9.  Genetic Encoding of bicyclononynes and trans-cyclooctenes for site-specific protein labeling in vitro and in live mammalian cells via rapid fluorogenic Diels-Alder reactions.

Authors:  Kathrin Lang; Lloyd Davis; Stephen Wallace; Mohan Mahesh; Daniel J Cox; Melissa L Blackman; Joseph M Fox; Jason W Chin
Journal:  J Am Chem Soc       Date:  2012-06-13       Impact factor: 15.419

10.  Expanding the genetic code of yeast for incorporation of diverse unnatural amino acids via a pyrrolysyl-tRNA synthetase/tRNA pair.

Authors:  Susan M Hancock; Rajendra Uprety; Alexander Deiters; Jason W Chin
Journal:  J Am Chem Soc       Date:  2010-10-27       Impact factor: 15.419
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  5 in total

1.  Hydrophilic 18F-labeled trans-5-oxocene (oxoTCO) for efficient construction of PET agents with improved tumor-to-background ratios in neurotensin receptor (NTR) imaging.

Authors:  Mengzhe Wang; Raghu Vannam; William D Lambert; Yixin Xie; Hui Wang; Benjamin Giglio; Xiaofen Ma; Zhanhong Wu; Joseph Fox; Zibo Li
Journal:  Chem Commun (Camb)       Date:  2019-02-21       Impact factor: 6.222

2.  Addition of Isocyanide-Containing Amino Acids to the Genetic Code for Protein Labeling and Activation.

Authors:  Yuda Chen; Kuan-Lin Wu; Juan Tang; Axel Loredo; Jordan Clements; Jingqi Pei; Zane Peng; Ruchi Gupta; Xinlei Fang; Han Xiao
Journal:  ACS Chem Biol       Date:  2019-11-14       Impact factor: 5.100

3.  Studies on the Stability and Stabilization of trans-Cyclooctenes through Radical Inhibition and Silver (I) Metal Complexation.

Authors:  Yinzhi Fang; Joshua C Judkins; Samantha J Boyd; Christopher W Am Ende; Katarina Rohlfing; Zhen Huang; Yixin Xie; Douglas S Johnson; Joseph M Fox
Journal:  Tetrahedron       Date:  2019-05-26       Impact factor: 2.457

4.  Bioorthogonal chemistry.

Authors:  Samuel L Scinto; Didier A Bilodeau; Robert Hincapie; Wankyu Lee; Sean S Nguyen; Minghao Xu; Christopher W Am Ende; M G Finn; Kathrin Lang; Qing Lin; John Paul Pezacki; Jennifer A Prescher; Marc S Robillard; Joseph M Fox
Journal:  Nat Rev Methods Primers       Date:  2021-04-15

5.  Concise Synthesis of Functionalized Cyclobutene Analogues for Bioorthogonal Tetrazine Ligation.

Authors:  Jiayu Sun; Jie Li; Hongbao Sun; Chunling Li; Haoxing Wu
Journal:  Molecules       Date:  2021-01-08       Impact factor: 4.411
  5 in total

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