Literature DB >> 22668517

Improved methodology for the affinity isolation of human protein complexes expressed at near endogenous levels.

Michal Domanski1, Kelly Molloy, Hua Jiang, Brian T Chait, Michael P Rout, Torben Heick Jensen, John LaCava.   

Abstract

An efficient and reliable procedure for the capture of affinity-tagged proteins and associated complexes from human cell lines is reported. Through multiple optimizations, high yield and low background affinity-purifications are achieved from modest quantities of human cells expressing endogenous-level tagged proteins. Isolations of triple-FLAG and GFP-tagged fusion proteins involved in RNA metabolism are presented.

Entities:  

Year:  2012        PMID： 22668517      PMCID： PMC3605737          DOI： 10.2144/000113864

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  46 in total

1.  ProFound: an expert system for protein identification using mass spectrometric peptide mapping information.

Authors:  W Zhang; B T Chait
Journal:  Anal Chem       Date:  2000-06-01       Impact factor: 6.986

2.  GPMAW--a software tool for analyzing proteins and peptides.

Authors:  S Peri; H Steen; A Pandey
Journal:  Trends Biochem Sci       Date:  2001-11       Impact factor: 13.807

3.  RADARS, a bioinformatics solution that automates proteome mass spectral analysis, optimises protein identification, and archives data in a relational database.

Authors:  Helen I Field; David Fenyö; Ronald C Beavis
Journal:  Proteomics       Date:  2002-01       Impact factor: 3.984

4.  Multiple tandem epitope tagging for enhanced detection of protein expressed in mammalian cells.

Authors:  L Zhang; R Hernan; B Brizzard
Journal:  Mol Biotechnol       Date:  2001-11       Impact factor: 2.695

Review 5.  The FLAG peptide, a versatile fusion tag for the purification of recombinant proteins.

Authors:  A Einhauer; A Jungbauer
Journal:  J Biochem Biophys Methods       Date:  2001-10-30

Review 6.  The eukaryotic RNA exosome: same scaffold but variable catalytic subunits.

Authors:  Søren Lykke-Andersen; Rafal Tomecki; Torben Heick Jensen; Andrzej Dziembowski
Journal:  RNA Biol       Date:  2011-01-01       Impact factor: 4.652

7.  AU binding proteins recruit the exosome to degrade ARE-containing mRNAs.

Authors:  C Y Chen; R Gherzi; S E Ong; E L Chan; R Raijmakers; G J Pruijn; G Stoecklin; C Moroni; M Mann; M Karin
Journal:  Cell       Date:  2001-11-16       Impact factor: 41.582

8.  A cap-binding protein complex mediating U snRNA export.

Authors:  E Izaurralde; J Lewis; C Gamberi; A Jarmolowski; C McGuigan; I W Mattaj
Journal:  Nature       Date:  1995-08-24       Impact factor: 49.962

9.  PHAX, a mediator of U snRNA nuclear export whose activity is regulated by phosphorylation.

Authors:  M Ohno; A Segref; A Bachi; M Wilm; I W Mattaj
Journal:  Cell       Date:  2000-04-14       Impact factor: 41.582

10.  Multiple epitope tagging of expressed proteins for enhanced detection.

Authors:  R Hernan; K Heuermann; B Brizzard
Journal:  Biotechniques       Date:  2000-04       Impact factor: 1.993
View more
  34 in total

Review 1.  The emerging role of native mass spectrometry in characterizing the structure and dynamics of macromolecular complexes.

Authors:  Elisabetta Boeri Erba; Carlo Petosa
Journal:  Protein Sci       Date:  2015-03-31       Impact factor: 6.725

2.  A pipeline for determining protein-protein interactions and proximities in the cellular milieu.

Authors:  Roman I Subbotin; Brian T Chait
Journal:  Mol Cell Proteomics       Date:  2014-08-29       Impact factor: 5.911

3.  Affinity proteomics to study endogenous protein complexes: pointers, pitfalls, preferences and perspectives.

Authors:  John LaCava; Kelly R Molloy; Martin S Taylor; Michal Domanski; Brian T Chait; Michael P Rout
Journal:  Biotechniques       Date:  2015-03-01       Impact factor: 1.993

4.  Combined proximity labeling and affinity purification-mass spectrometry workflow for mapping and visualizing protein interaction networks.

Authors:  Xiaonan Liu; Kari Salokas; Rigbe G Weldatsadik; Lisa Gawriyski; Markku Varjosalo
Journal:  Nat Protoc       Date:  2020-08-10       Impact factor: 13.491

5.  Characterization of L1-Ribonucleoprotein Particles.

Authors:  Martin S Taylor; John LaCava; Lixin Dai; Paolo Mita; Kathleen H Burns; Michael P Rout; Jef D Boeke
Journal:  Methods Mol Biol       Date:  2016

6.  Determining the Composition and Stability of Protein Complexes Using an Integrated Label-Free and Stable Isotope Labeling Strategy.

Authors:  Todd M Greco; Amanda J Guise; Ileana M Cristea
Journal:  Methods Mol Biol       Date:  2016

7.  Nol12 is a multifunctional RNA binding protein at the nexus of RNA and DNA metabolism.

Authors:  Daniel D Scott; Christian Trahan; Pierre J Zindy; Lisbeth C Aguilar; Marc Y Delubac; Eric L Van Nostrand; Srivathsan Adivarahan; Karen E Wei; Gene W Yeo; Daniel Zenklusen; Marlene Oeffinger
Journal:  Nucleic Acids Res       Date:  2017-12-01       Impact factor: 16.971

Review 8.  Proteomics-based methods for discovery, quantification, and validation of protein-protein interactions.

Authors:  Yana V Miteva; Hanna G Budayeva; Ileana M Cristea
Journal:  Anal Chem       Date:  2012-12-12       Impact factor: 6.986

9.  Affinity proteomics reveals human host factors implicated in discrete stages of LINE-1 retrotransposition.

Authors:  Martin S Taylor; John LaCava; Paolo Mita; Kelly R Molloy; Cheng Ran Lisa Huang; Donghui Li; Emily M Adney; Hua Jiang; Kathleen H Burns; Brian T Chait; Michael P Rout; Jef D Boeke; Lixin Dai
Journal:  Cell       Date:  2013-11-21       Impact factor: 41.582

10.  A Robust Workflow for Native Mass Spectrometric Analysis of Affinity-Isolated Endogenous Protein Assemblies.

Authors:  Paul Dominic B Olinares; Amelia D Dunn; Júlio C Padovan; Javier Fernandez-Martinez; Michael P Rout; Brian T Chait
Journal:  Anal Chem       Date:  2016-02-18       Impact factor: 6.986
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.