Literature DB >> 11694294

The FLAG peptide, a versatile fusion tag for the purification of recombinant proteins.

A Einhauer1, A Jungbauer.   

Abstract

A fusion tag, called FLAG and consisting of eight amino acids (AspTyrLysAspAspAspAspLys) including an enterokinase-cleavage site, was specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing conditions [Bio/Technology, 6 (1988) 1204]. Several antibodies against this peptide have been developed. One antibody, denoted as M1, binds the peptide in the presence of bivalent metal cations, preferably Ca(+). Elution is effected by chelating agents. Another strategy is competitive elution with excess of free FLAG peptide. Antibodies M2 and M5 are applied in this procedure. Examples demonstrating the versatility, practicability and limitations of this technology are given.

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Year:  2001        PMID： 11694294     DOI： 10.1016/s0165-022x(01)00213-5

Source DB:  PubMed          Journal:  J Biochem Biophys Methods        ISSN： 0165-022X


  122 in total

1.  Improved methodology for the affinity isolation of human protein complexes expressed at near endogenous levels.

Authors:  Michal Domanski; Kelly Molloy; Hua Jiang; Brian T Chait; Michael P Rout; Torben Heick Jensen; John LaCava
Journal:  Biotechniques       Date:  2012-05       Impact factor: 1.993

2.  Rapid and sensitive identification of epitope-containing peptides by direct matrix-assisted laser desorption/ionization tandem mass spectrometry of peptides affinity-bound to antibody beads.

Authors:  Christina S Raska; Carol E Parker; Susan W Sunnarborg; R Marshall Pope; David C Lee; Gary L Glish; Christoph H Borchers
Journal:  J Am Soc Mass Spectrom       Date:  2003-10       Impact factor: 3.109

3.  DNA display for in vitro selection of diverse peptide libraries.

Authors:  Masato Yonezawa; Nobuhide Doi; Yuko Kawahashi; Toru Higashinakagawa; Hiroshi Yanagawa
Journal:  Nucleic Acids Res       Date:  2003-10-01       Impact factor: 16.971

4.  In vivo immobilization of fusion proteins on bioplastics by the novel tag BioF.

Authors:  Cristina Moldes; Pedro García; José L García; María A Prieto
Journal:  Appl Environ Microbiol       Date:  2004-06       Impact factor: 4.792

5.  A highly efficient multifunctional tandem affinity purification approach applicable to diverse organisms.

Authors:  Hanhui Ma; Janel R McLean; Lucy Fang-I Chao; Sebastian Mana-Capelli; Murugan Paramasivam; Kirsten A Hagstrom; Kathleen L Gould; Dannel McCollum
Journal:  Mol Cell Proteomics       Date:  2012-04-03       Impact factor: 5.911

Review 6.  Tools used to study how protein complexes are assembled in signaling cascades.

Authors:  Susan Dwane; Patrick A Kiely
Journal:  Bioeng Bugs       Date:  2011-09-01

7.  Homo-oligomerization of Marburgvirus VP35 is essential for its function in replication and transcription.

Authors:  Peggy Möller; Nonia Pariente; Hans-Dieter Klenk; Stephan Becker
Journal:  J Virol       Date:  2005-12       Impact factor: 5.103

8.  Toward mosquito control with a green alga: Expression of Cry toxins of Bacillus thuringiensis subsp. israelensis (Bti) in the chloroplast of Chlamydomonas.

Authors:  Seongjoon Kang; Obed W Odom; Saravanan Thangamani; David L Herrin
Journal:  J Appl Phycol       Date:  2016-11-23       Impact factor: 3.215

9.  Identification of AGO3-associated miRNAs and computational prediction of their targets in the green alga Chlamydomonas reinhardtii.

Authors:  Adam Voshall; Eun-Jeong Kim; Xinrong Ma; Etsuko N Moriyama; Heriberto Cerutti
Journal:  Genetics       Date:  2015-03-13       Impact factor: 4.562

10.  DivL performs critical cell cycle functions in Caulobacter crescentus independent of kinase activity.

Authors:  Sarah J Reisinger; Sarah Huntwork; Patrick H Viollier; Kathleen R Ryan
Journal:  J Bacteriol       Date:  2007-09-07       Impact factor: 3.490
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