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Literature DB >> 22488831

Triple-color super-resolution imaging of live cells: resolving submicroscopic receptor organization in the plasma membrane.

Stephan Wilmes¹, Markus Staufenbiel, Domenik Lisse, Christian P Richter, Oliver Beutel, Karin B Busch, Samuel T Hess, Jacob Piehler.

Abstract

In living color: efficient intracellular covalent labeling of proteins with a photoswitchable dye using the HaloTag for dSTORM super-resolution imaging in live cells is described. The dynamics of cellular nanostructures at the plasma membrane were monitored with a time resolution of a few seconds. In combination with dual-color FPALM imaging, submicroscopic receptor organization within the context of the membrane skeleton was resolved.

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Year: 2012 PMID： 22488831 DOI： 10.1002/anie.201200853

Source DB: PubMed Journal: Angew Chem Int Ed Engl ISSN： 1433-7851 Impact factor: 15.336

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Cited

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