Literature DB >> 24782148

Far-red organic fluorophores contain a fluorescent impurity.

Matthew B Stone1, Sarah L Veatch.   

Abstract

Far-red organic fluorophores commonly used in traditional and super-resolution localization microscopy are found to contain a fluorescent impurity with green excitation and near-red emission. This near-red fluorescent impurity can interfere with some multicolor stochastic optical reconstruction microscopy/photoactivated localization microscopy measurements in live cells and produce subtle artifacts in chemically fixed cells. We additionally describe alternatives to avoid artifacts in super-resolution localization microscopy.
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Keywords:  fluorescence; fluorescent probes; live cells; quantitative imaging; super-resolution imaging

Mesh：

Substances：

Year:  2014        PMID： 24782148      PMCID： PMC4180537          DOI： 10.1002/cphc.201402002

Source DB:  PubMed          Journal:  Chemphyschem        ISSN： 1439-4235            Impact factor:   3.102


  31 in total

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  9 in total

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