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Literature DB >> 2216769

Chromatin assembly on replicating DNA in vitro.

G Almouzni¹, D J Clark, M Méchali, A P Wolffe.

Abstract

Replicating single-stranded DNA is preferentially assembled into chromatin in Xenopus egg extracts relative to non-replicating double-stranded DNA. We have examined the molecular basis of this phenomenon. Single-stranded DNA itself is not a favored template for nucleosome assembly in comparison to double-stranded DNA. Complementary strand synthesis is required for the rapid assembly of nucleosomes. We present evidence that the assembly of chromatin on replicating DNA is a two step phenomenon. The first step involves the replication of DNA and the assembly of an intermediate structure, the second step involves the sequestration of histones H2A/H2B onto DNA. Histones H2A/H2B are preferentially sequestered onto replicated DNA in comparison to non-replicated DNA incubated in the extract.

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Year: 1990 PMID： 2216769 PMCID： PMC332312 DOI： 10.1093/nar/18.19.5767

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

31 in total

1. Assembly of newly replicated chromatin.

Authors: A Worcel; S Han; M L Wong
Journal: Cell Date: 1978-11 Impact factor: 41.582

2. H3.H4 tetramer directs DNA and core histone octamer assembly in the nucleosome core particle.

Authors: J L Jorcano; A Ruiz-Carrillo
Journal: Biochemistry Date: 1979-03-06 Impact factor: 3.162

3. Folding of the DNA double helix in chromatin-like structures from simian virus 40.

Authors: J E Germond; B Hirt; P Oudet; M Gross-Bellark; P Chambon
Journal: Proc Natl Acad Sci U S A Date: 1975-05 Impact factor: 11.205

4. The problems of eukaryotic and prokaryotic DNA packaging and in vivo conformation posed by superhelix density heterogeneity.

Authors: M Shure; D E Pulleyblank; J Vinograd
Journal: Nucleic Acids Res Date: 1977 Impact factor: 16.971

5. Specific folding and contraction of DNA by histones H3 and H4.

Authors: M Bina-Stein; R T Simpson
Journal: Cell Date: 1977-07 Impact factor: 41.582

6. Negative supercoiling is not required for 5S RNA transcription in vitro.

Authors: A P Wolffe; M T Andrews; E Crawford; R Losa; D D Brown
Journal: Cell Date: 1987-05-08 Impact factor: 41.582

7. Supercoiling energy and nucleosome formation: the role of the arginine-rich histone kernel.

Authors: R D Camerini-Otero; G Felsenfeld
Journal: Nucleic Acids Res Date: 1977 Impact factor: 16.971

8. A new procedure for purifying histone pairs H2A + H2B and H3 + H4 from chromatin using hydroxylapatite.

Authors: R H Simon; G Felsenfeld
Journal: Nucleic Acids Res Date: 1979-02 Impact factor: 16.971

9. Preferential association of newly synthesized H3 and H4 histones with newly replicated DNA.

Authors: T Senshu; M Fukuda; M Ohashi
Journal: J Biochem Date: 1978-10 Impact factor: 3.387

10. A nuclear extract of Xenopus laevis oocytes that accurately transcribes 5S RNA genes.

Authors: E H Birkenmeier; D D Brown; E Jordan
Journal: Cell Date: 1978-11 Impact factor: 41.582

33 in total

1. Fast kinetics of chromatin assembly revealed by single-molecule videomicroscopy and scanning force microscopy.

Authors: B Ladoux; J P Quivy; P Doyle; O du Roure; G Almouzni; J L Viovy
Journal: Proc Natl Acad Sci U S A Date: 2000-12-19 Impact factor: 11.205

Review 2. Role of histone acetylation in the assembly and modulation of chromatin structures.

Authors: A T Annunziato; J C Hansen
Journal: Gene Expr Date: 2000

3. Chromatin disruption and histone acetylation in regulation of the human immunodeficiency virus type 1 long terminal repeat by thyroid hormone receptor.

Authors: Shao-Chung Victor Hsia; Yun-Bo Shi
Journal: Mol Cell Biol Date: 2002-06 Impact factor: 4.272

Chromatin assembly on replicating DNA in vitro.

1. Assembly of newly replicated chromatin.

2. H3.H4 tetramer directs DNA and core histone octamer assembly in the nucleosome core particle.

3. Folding of the DNA double helix in chromatin-like structures from simian virus 40.

4. The problems of eukaryotic and prokaryotic DNA packaging and in vivo conformation posed by superhelix density heterogeneity.

5. Specific folding and contraction of DNA by histones H3 and H4.

6. Negative supercoiling is not required for 5S RNA transcription in vitro.

7. Supercoiling energy and nucleosome formation: the role of the arginine-rich histone kernel.

8. A new procedure for purifying histone pairs H2A + H2B and H3 + H4 from chromatin using hydroxylapatite.

9. Preferential association of newly synthesized H3 and H4 histones with newly replicated DNA.

10. A nuclear extract of Xenopus laevis oocytes that accurately transcribes 5S RNA genes.

1. Fast kinetics of chromatin assembly revealed by single-molecule videomicroscopy and scanning force microscopy.

Review 2. Role of histone acetylation in the assembly and modulation of chromatin structures.

3. Chromatin disruption and histone acetylation in regulation of the human immunodeficiency virus type 1 long terminal repeat by thyroid hormone receptor.

4. Cell-free system for assembly of transcriptionally repressed chromatin from Drosophila embryos.

5. Histone contributions to the structure of DNA in the nucleosome.

Review 6. Relationship of eukaryotic DNA replication to committed gene expression: general theory for gene control.

7. Transcription complex disruption caused by a transition in chromatin structure.

8. Human TFIIIA alone is sufficient to prevent nucleosomal repression of a homologous 5S gene.

Review 9. Chaperone-mediated chromatin assembly and transcriptional regulation in Xenopus laevis.

10. Histone H1 deposition and histone-DNA interactions in replicating chromatin.