Literature DB >> 424310

A new procedure for purifying histone pairs H2A + H2B and H3 + H4 from chromatin using hydroxylapatite.

R H Simon, G Felsenfeld.   

Abstract

A method to purify histone groups H2A+H2B and H3+H4 using dissociation with NaCl and hydroxylapatite chromatography is presented. The procedure is simple, involves mild solvents, and provides milligram quantities of histones of high purity. The histone pairs prepared by this method can regenerate chromatin-like characteristics when combined and reconstituted with DNA.

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Year:  1979        PMID: 424310      PMCID: PMC327721          DOI: 10.1093/nar/6.2.689

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN: 0305-1048            Impact factor:   16.971


  14 in total

1.  A new procedure for the isolation and fractionation of histones.

Authors:  D R. van der Westhuyzen; C von Holt
Journal:  FEBS Lett       Date:  1971-05-20       Impact factor: 4.124

2.  Thymus deoxyribonucleoprotein. III. Sedimentation behaviour.

Authors:  G GIANNONI; A R PEACOCKE
Journal:  Biochim Biophys Acta       Date:  1963-02-26

3.  Thymus deoxyribonucleoprotein. II. Dissociation in sodium chloride solution.

Authors:  P M BAYLEY; B N PRESTON; A R PEACOCKE
Journal:  Biochim Biophys Acta       Date:  1962-06-11

4.  Physical studies of chromatin. The recombination of histones with DNA.

Authors:  P G Boseley; E M Bradbury; G S Butler-Browne; B G Carpenter; R M Stephens
Journal:  Eur J Biochem       Date:  1976-02-02

5.  Specific folding and contraction of DNA by histones H3 and H4.

Authors:  M Bina-Stein; R T Simpson
Journal:  Cell       Date:  1977-07       Impact factor: 41.582

6.  The organization of histones and DNA in chromatin: evidence for an arginine-rich histone kernel.

Authors:  R D Camerini-Otero; B Sollner-Webb; G Felsenfeld
Journal:  Cell       Date:  1976-07       Impact factor: 41.582

7.  Chromatin structure as probed by nucleases and proteases: evidence for the central role of histones H3 and H4.

Authors:  B Sollner-Webb; R D Camerini-Otero; G Felsenfeld
Journal:  Cell       Date:  1976-09       Impact factor: 41.582

8.  Selective dissociation of histones from calf thymus nucleoprotein.

Authors:  H H Ohlenbusch; B M Olivera; D Tuan; N Davidson
Journal:  J Mol Biol       Date:  1967-04-28       Impact factor: 5.469

9.  Synthesis of globin ribonucleic acid from duck-reticulocyte chromatin in vitro.

Authors:  R Axel; H Cedar; G Felsenfeld
Journal:  Proc Natl Acad Sci U S A       Date:  1973-07       Impact factor: 11.205

10.  Structure of nucleohistone. I. Hydrodynamic behaviour.

Authors:  Y Ohba
Journal:  Biochim Biophys Acta       Date:  1966-07-20
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  110 in total

1.  Fast kinetics of chromatin assembly revealed by single-molecule videomicroscopy and scanning force microscopy.

Authors:  B Ladoux; J P Quivy; P Doyle; O du Roure; G Almouzni; J L Viovy
Journal:  Proc Natl Acad Sci U S A       Date:  2000-12-19       Impact factor: 11.205

2.  Epstein-Barr nuclear antigen 1 binds and destabilizes nucleosomes at the viral origin of latent DNA replication.

Authors:  T M Avolio-Hunter; P N Lewis; L Frappier
Journal:  Nucleic Acids Res       Date:  2001-09-01       Impact factor: 16.971

3.  Reconstitution of enhancer function in paternal pronuclei of one-cell mouse embryos.

Authors:  L Rastelli; K Robinson; Y Xu; S Majumder
Journal:  Mol Cell Biol       Date:  2001-08       Impact factor: 4.272

4.  Histone acetylation and histone deacetylation.

Authors:  Kazuhiro Ito; Ian M Adcock
Journal:  Mol Biotechnol       Date:  2002-01       Impact factor: 2.695

5.  DNA base excision repair of uracil residues in reconstituted nucleosome core particles.

Authors:  Hilde Nilsen; Tomas Lindahl; Alain Verreault
Journal:  EMBO J       Date:  2002-11-01       Impact factor: 11.598

6.  Chromatin assembly factor 1 is essential and couples chromatin assembly to DNA replication in vivo.

Authors:  Maarten Hoek; Bruce Stillman
Journal:  Proc Natl Acad Sci U S A       Date:  2003-09-30       Impact factor: 11.205

7.  Structural features of transcription factor IIIA bound to a nucleosome in solution.

Authors:  Joseph M Vitolo; Zungyoon Yang; Ravi Basavappa; Jeffrey J Hayes
Journal:  Mol Cell Biol       Date:  2004-01       Impact factor: 4.272

8.  Unexpected binding motifs for subnucleosomal particles revealed by atomic force microscopy.

Authors:  Dessy N Nikova; Lisa H Pope; Martin L Bennink; Kirsten A van Leijenhorst-Groener; Kees van der Werf; Jan Greve
Journal:  Biophys J       Date:  2004-09-17       Impact factor: 4.033

9.  Periodic binding of individual core histones to DNA: inadvertent purification of the core histone H2B as a putative enhancer-binding factor.

Authors:  L A Kerrigan; J T Kadonaga
Journal:  Nucleic Acids Res       Date:  1992-12-25       Impact factor: 16.971

10.  ACF1 improves the effectiveness of nucleosome mobilization by ISWI through PHD-histone contacts.

Authors:  Anton Eberharter; Irene Vetter; Roger Ferreira; Peter B Becker
Journal:  EMBO J       Date:  2004-09-30       Impact factor: 11.598

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