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Literature DB >> 711710

Preferential association of newly synthesized H3 and H4 histones with newly replicated DNA.

Abstract

The assembly of newly synthesized histones into chromatin during replication of MH-134SC cells was studied. Cells pulse-labeled with iododeoxyuridine and [3H]lysine were mixed with an equivalent number of normal cells labeled with [14C]lysine. Nuclease chromatin obtained from pooled cells was fractionated by buoyant-density centrifugation in a gradient containing Metrizamide and 3-iodo-1,2-propanediol. Histones extracted from heavy and normal chromatin regions of the gradient were fractionated by acid-urea gel electrophoresis, and 3H/14C ratios of individual histones were compared. The results showed highly preferential association of newly synthesized H3 and H4 with newly replicated DNA.

Entities: Chemical

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Year: 1978 PMID： 711710 DOI： 10.1093/oxfordjournals.jbchem.a132213

Source DB: PubMed Journal: J Biochem ISSN： 0021-924X Impact factor: 3.387

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Cited

17 in total

1. Fast kinetics of chromatin assembly revealed by single-molecule videomicroscopy and scanning force microscopy.

Authors: B Ladoux; J P Quivy; P Doyle; O du Roure; G Almouzni; J L Viovy
Journal: Proc Natl Acad Sci U S A Date: 2000-12-19 Impact factor: 11.205

2. Chromatin assembly on replicating DNA in vitro.

Authors: G Almouzni; D J Clark; M Méchali; A P Wolffe
Journal: Nucleic Acids Res Date: 1990-10-11 Impact factor: 16.971

3. Histones H2A/H2B inhibit the interaction of transcription factor IIIA with the Xenopus borealis somatic 5S RNA gene in a nucleosome.

Authors: J J Hayes; A P Wolffe
Journal: Proc Natl Acad Sci U S A Date: 1992-02-15 Impact factor: 11.205

4. Multistep pathway for replication-dependent nucleosome assembly.

Authors: R Fotedar; J M Roberts
Journal: Proc Natl Acad Sci U S A Date: 1989-09 Impact factor: 11.205

5. Characterization of the repressed 5S DNA minichromosomes assembled in vitro with a high-speed supernatant of Xenopus laevis oocytes.

Authors: A Shimamura; D Tremethick; A Worcel
Journal: Mol Cell Biol Date: 1988-10 Impact factor: 4.272

6. Formation of transcribing mononucleosome-eukaryotic RNA polymerase II complexes in vitro as a simple model of active chromatin.

Authors: K Sakuma; Y Matsumura; T Senshu
Journal: Nucleic Acids Res Date: 1984-02-10 Impact factor: 16.971

Preferential association of newly synthesized H3 and H4 histones with newly replicated DNA.

1. Fast kinetics of chromatin assembly revealed by single-molecule videomicroscopy and scanning force microscopy.

2. Chromatin assembly on replicating DNA in vitro.

3. Histones H2A/H2B inhibit the interaction of transcription factor IIIA with the Xenopus borealis somatic 5S RNA gene in a nucleosome.

4. Multistep pathway for replication-dependent nucleosome assembly.

5. Characterization of the repressed 5S DNA minichromosomes assembled in vitro with a high-speed supernatant of Xenopus laevis oocytes.

6. Formation of transcribing mononucleosome-eukaryotic RNA polymerase II complexes in vitro as a simple model of active chromatin.

Review 7. Chromatin replication, reconstitution and assembly.

8. Assembly of new histones into nucleosomes and their distribution in replicating chromatin.

9. Distribution of the core histones H2A.H2B.H3 and H4 during cell replication.

10. S-phase-dependent action of cycloheximide in relieving chromatin-mediated general transcriptional repression.