Literature DB >> 22081366

Genetic characterization of Escherichia coli O157:H7 strains isolated from the one-humped camel (Camelus dromedarius) by using microarray DNA technology.

Taghi Zahraei Salehi1, Alfreda Tonelli, Alberto Mazza, Hamid Staji, Pietro Badagliacca, Iradj Ashrafi Tamai, Reza Jamshidi, Josée Harel, Rossella Lelli, Luke Masson.   

Abstract

From the Camelidae family members, several serotypes of Escherichia coli (E. coli) have recently been isolated from diarrhoeic and non-diarrhoeic faecal samples. To date Shiga toxin-producing E. coli (STEC) strains have never been typed in one-humped camel (Camelus dromedarius). In the present study, two E. coli O157:H7 strains isolated from sick dromedaries were investigated. Virulence gene profiles were determined using a custom E. coli virulence DNA microarray, composed of 70-mer oligonucleotide probes targeting 264 virulence or related genes of known E. coli pathotypes. Both strains displayed positive hybridization signals for the Locus of enterocyte effacement (LEE) gene probes (ler, eae, espA, espB, tir genes), two Shiga toxin probes (stx1 and stx2), the O157 O-antigen specific probe, various virulence plasmid (pO157) probes like katP in addition to other accessory virulence genes characterized in STEC.

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Year:  2012        PMID： 22081366     DOI： 10.1007/s12033-011-9466-7

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  26 in total

1.  Virulence gene profiles and intimin subtypes of Shiga toxin-producing Escherichia coli isolated from healthy and diarrhoeic calves.

Authors:  M Askari Badouei; T Zahraei Salehi; M Rabbani Khorasgani; H Tadjbakhsh; G Nikbakht Brujeni; M G Nadalian
Journal:  Vet Rec       Date:  2010-11-27       Impact factor: 2.695

2.  Identification & characterization of Shiga toxin-producing Escherichia coli isolates from patients with diarrhoea in Iran.

Authors:  M Bonyadian; H Momtaz; E Rahimi; R Habibian; A Yazdani; M Zamani
Journal:  Indian J Med Res       Date:  2010-09       Impact factor: 2.375

3.  Characteristics of the enteroaggregative Shiga toxin/verotoxin-producing Escherichia coli O104:H4 strain causing the outbreak of haemolytic uraemic syndrome in Germany, May to June 2011.

Authors:  F Scheutz; E Møller Nielsen; J Frimodt-Møller; N Boisen; S Morabito; R Tozzoli; J P Nataro; A Caprioli
Journal:  Euro Surveill       Date:  2011-06-16

4.  Development and validation of an oligonucleotide microarray for detection of multiple virulence and antimicrobial resistance genes in Escherichia coli.

Authors:  Guillaume Bruant; Christine Maynard; Sadjia Bekal; Isabelle Gaucher; Luke Masson; Roland Brousseau; Josée Harel
Journal:  Appl Environ Microbiol       Date:  2006-05       Impact factor: 4.792

Review 5.  Enterohaemorrhagic E. coli in veterinary medicine.

Authors:  Stuart W Naylor; David L Gally; J Christopher Low
Journal:  Int J Med Microbiol       Date:  2005-10       Impact factor: 3.473

6.  Prevalence of faecal pathogens in calves of racing camels (Camelus dromedarius) in the United Arab Emirates.

Authors:  J E Moore; M McCalmont; Jiru Xu; G Nation; A H Tinson; L Crothers; D W G Harron
Journal:  Trop Anim Health Prod       Date:  2002-07       Impact factor: 1.559

7.  Sporadic bloody diarrhoea-associated thrombotic thrombocytopenic purpura-haemolytic uraemic syndrome: an adult and paediatric comparison.

Authors:  Charity A Karpac; Xiaoning Li; Deirdra R Terrell; Johanna A Kremer Hovinga; Bernhard Lämmle; Sara K Vesely; James N George
Journal:  Br J Haematol       Date:  2008-04-15       Impact factor: 6.998

Review 8.  Infection by Shiga toxin-producing Escherichia coli: an overview.

Authors:  Mohamed A Karmali
Journal:  Mol Biotechnol       Date:  2004-02       Impact factor: 2.695

Review 9.  Pathogenic Escherichia coli.

Authors:  James B Kaper; James P Nataro; Harry L Mobley
Journal:  Nat Rev Microbiol       Date:  2004-02       Impact factor: 60.633

10.  Genome evolution in major Escherichia coli O157:H7 lineages.

Authors:  Yongxiang Zhang; Chad Laing; Marina Steele; Kim Ziebell; Roger Johnson; Andrew K Benson; Eduardo Taboada; Victor P J Gannon
Journal:  BMC Genomics       Date:  2007-05-16       Impact factor: 3.969
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  5 in total

1.  Survey on O157:H7 enterohemorrhagic Escherichia coli (EHEC) in cattle in Golestan province, Iran.

Authors:  Alireza Koochakzadeh; Mahdi Askari Badouei; Emad Mazandarani; Omid Madadgar
Journal:  Iran J Microbiol       Date:  2014-08

2.  Distribution of Shiga toxin genes subtypes in B1 phylotypes of Escherichia coli isolated from calves suffering from diarrhea in Tehran suburb using DNA oligonucleotide arrays.

Authors:  Hamid Staji; Alfreda Tonelli; Abbas Javaheri-Vayeghan; Emad Changizi; Mohammad Reza Salimi-Bejestani
Journal:  Iran J Microbiol       Date:  2015-08

3.  DNA Microarray for Rapid Detection and Identification of Food and Water Borne Bacteria: From Dry to Wet Lab.

Authors:  Reza Ranjbar; Payam Behzadi; Ali Najafi; Raheleh Roudi
Journal:  Open Microbiol J       Date:  2017-11-30

4.  Comparative virulotyping and phylogenomics of Escherichia coli isolates from urine samples of men and women suffering urinary tract infections.

Authors:  Hamid Staji; Maryam Rassouli; Samaneh Jourablou
Journal:  Iran J Basic Med Sci       Date:  2019-02       Impact factor: 2.699

5.  Shiga toxin-producing Escherichia coli (STEC) isolated from fecal samples of African dromedary camels.

Authors:  Melinda Baschera; Nicole Cernela; Marc J A Stevens; Anne Liljander; Jörg Jores; Victor Max Corman; Magdalena Nüesch-Inderbinen; Roger Stephan
Journal:  One Health       Date:  2019-03-07
  5 in total

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