BACKGROUND & OBJECTIVES: Verotoxigenic Escherichia coli are important serotypes of enterohaemorrhagic E. coli (EHEC) subgroup that cause attaching and effacing lesions in enterocytes by producing verotoxins or shiga-like toxins resulting in haemorrhagic colitis (HC) and haemolytic uremic syndrome (HUS). The aim of this study was to detect these serotypes specially E. coli O157:H7 in stool samples of patients with diarrhoea and identification of virulence genes (STX1, STX2, Hly and EAE) in Shahrekord-Iran area using PCR technique. METHODS: Two hundred diarrhoeal stool samples of patients were collected through 2007-2008. Microbiological and biochemical examinations were done to detect the E. coli. Serological tests carried out to identify the O157 or O157:H7 serotypes. RESULTS: Of the 58 E. coli isolates, 16 (27.6%) were detected as STX1 carrying E. coli, four (6.9%) carrying STX2, eight (13.8%) carrying both STX1 and STX2, and 12 (20.7%) were Hly carrying E. coli, but none of the isolates contained EAE gene. None of the isolates were E. coli O157 or O157:H7 serotypes. INTERPRETATION & CONCLUSIONS: Our results revealed that verotoxigenic E. coli isolates other than O157 serotype were involved in causing diarrhoea in Shahrekord-Iran.
BACKGROUND & OBJECTIVES: Verotoxigenic Escherichia coli are important serotypes of enterohaemorrhagic E. coli (EHEC) subgroup that cause attaching and effacing lesions in enterocytes by producing verotoxins or shiga-like toxins resulting in haemorrhagic colitis (HC) and haemolytic uremic syndrome (HUS). The aim of this study was to detect these serotypes specially E. coli O157:H7 in stool samples of patients with diarrhoea and identification of virulence genes (STX1, STX2, Hly and EAE) in Shahrekord-Iran area using PCR technique. METHODS: Two hundred diarrhoeal stool samples of patients were collected through 2007-2008. Microbiological and biochemical examinations were done to detect the E. coli. Serological tests carried out to identify the O157 or O157:H7 serotypes. RESULTS: Of the 58 E. coli isolates, 16 (27.6%) were detected as STX1 carrying E. coli, four (6.9%) carrying STX2, eight (13.8%) carrying both STX1 and STX2, and 12 (20.7%) were Hly carrying E. coli, but none of the isolates contained EAE gene. None of the isolates were E. coli O157 or O157:H7 serotypes. INTERPRETATION & CONCLUSIONS: Our results revealed that verotoxigenic E. coli isolates other than O157 serotype were involved in causing diarrhoea in Shahrekord-Iran.
Authors: Mohammad Taghi Akhi; Ali Toloue Ostadgavahi; Reza Ghotaslou; Mohammad Asgharzadeh; Tahereh Pirzadeh; Vida Sorayaei Sowmesarayi; Mohammad Yousef Memar Journal: Jundishapur J Microbiol Date: 2015-11-21 Impact factor: 0.747
Authors: Ali Konaté; René Dembélé; Assèta Kagambèga; Issiaka Soulama; Wendpoulomdé A D Kaboré; Emmanuel Sampo; Haoua Cissé; Antoine Sanou; Samuel Serme; Soumanaba Zongo; Cheikna Zongo; Alio Mahamadou Fody; Nathalie K Guessennd; Alfred S Traoré; Amy Gassama-Sow; Nicolas Barro Journal: Eur J Microbiol Immunol (Bp) Date: 2017-08-09